1. Membrane Transporter/Ion Channel
  2. P2X Receptor
  3. BzATP triethylammonium salt

BzATP triethylammonium salt acts as a P2X receptor agonist with pEC50s of 8.74, 5.26, 7.10, 7.50, 6.19, 6.31, 5.33 for P2X1, P2X2, P2X3, P2X2/3, P2X4 and P2X7, respectively. BzATP triethylammonium salt is potent at P2X7 receptors with EC50s of 3.6 μM and 285 μM for rat P2X7 and mouse P2X7, respectively.

For research use only. We do not sell to patients.

BzATP triethylammonium salt Chemical Structure

BzATP triethylammonium salt Chemical Structure

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5 mg USD 120 In-stock
10 mg USD 190 In-stock
25 mg USD 390 In-stock
50 mg USD 680 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BzATP triethylammonium salt acts as a P2X receptor agonist with pEC50s of 8.74, 5.26, 7.10, 7.50, 6.19, 6.31, 5.33 for P2X1, P2X2, P2X3, P2X2/3, P2X4 and P2X7, respectively[1]. BzATP triethylammonium salt is potent at P2X7 receptors with EC50s of 3.6 μM and 285 μM for rat P2X7 and mouse P2X7, respectively[2].

IC50 & Target

p2x1 Receptor

 

P2X3 Receptor

 

P2X4 Receptor

 

P2X7 Receptor

 

In Vitro

BzATP (10-1000 μM; 24 h) promotes the proliferation and migration of U87 and U251 glioma cells[3].
P2X7R protein expression is induced by BzATP (100 μM; 6-48 h) in human glioma cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[3]

Cell Line: U87 and U251 glioma cells
Concentration: 5, 10, 50, 100, 500 and 1000 μM
Incubation Time: 2, 6, 12, 24, 48 and 72 hours
Result: The proliferation of U87 and U251 glioma cell lines was significantly increased in the presence of 10-1000 uM and 100-1000 μM, respectively.
The peak of cell proliferation of both U87 and U251 cell lines was at 100 μM.
The optimal incubation time is 24 hours in both U87 and U251 cells lines.

Western Blot Analysis[3]

Cell Line: U87 and U251 glioma cells
Concentration: 100 μM
Incubation Time: 6-48 hours
Result: Induced the upregulation of P2X7R.
In Vivo

BzATP (5 mg/kg) significantly promotes P2X7R expression in the intestines compared with intestines in the sham group and the control group after cecal ligation and puncture (CLP) induction[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male 2-month-old C57BL/6 mice (each weighing between 20 and 25 g)[4]
Dosage: 5 mg/kg
Administration: Injected through the intraperitoneal route
Result: At 48 hours, mice in the treated group and control group exhibited mortalities of 91% and 86%, respectively.
Molecular Weight

1018.97

Formula

C24H24N5O15P3.C18H45N3

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1[C@H](O[C@H]([C@@H]1OC(C(C=C2)=CC=C2C(C3=CC=CC=C3)=O)=O)N4C5=NC=NC(N)=C5N=C4)COP(OP(OP([O-])(O)=O)([O-])=O)([O-])=O.O=C(O[C@@H]6[C@H](O[C@H]([C@@H]6O)N7C8=NC=NC(N)=C8N=C7)COP(OP(OP([O-])(O)=O)([O-])=O)([O-])=O)C(C=C9)=CC=C9C(C%10=CC=CC=C%10)=O.CC[NH+](CC)CC.CC[NH+](CC)CC.CC[NH+](CC)CC.CC[NH+](CC)CC.CC[NH+](CC)CC.CC[NH+](CC)CC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (49.07 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9814 mL 4.9069 mL 9.8138 mL
5 mM 0.1963 mL 0.9814 mL 1.9628 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (98.14 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥95.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.9814 mL 4.9069 mL 9.8138 mL 24.5346 mL
5 mM 0.1963 mL 0.9814 mL 1.9628 mL 4.9069 mL
10 mM 0.0981 mL 0.4907 mL 0.9814 mL 2.4535 mL
15 mM 0.0654 mL 0.3271 mL 0.6543 mL 1.6356 mL
20 mM 0.0491 mL 0.2453 mL 0.4907 mL 1.2267 mL
25 mM 0.0393 mL 0.1963 mL 0.3926 mL 0.9814 mL
30 mM 0.0327 mL 0.1636 mL 0.3271 mL 0.8178 mL
40 mM 0.0245 mL 0.1227 mL 0.2453 mL 0.6134 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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BzATP triethylammonium salt Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BzATP triethylammonium salt
Cat. No.:
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