2. Metabolic Enzyme/Protease
  3. Cathepsin
  4. Bz-FVR-AMC

Bz-FVR-AMC is a fluorogenic substrate for procathepsin with a kcat/Km value of 1070 mM-1s-1. The high concentration of BZ-FVR-AMC inhibits the substrate.

For research use only. We do not sell to patients.

Bz-FVR-AMC Chemical Structure

Bz-FVR-AMC Chemical Structure

CAS No. : 88899-22-3

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5 mg USD 255 In-stock
10 mg USD 429 In-stock
25 mg USD 750 In-stock
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Based on 1 publication(s) in Google Scholar

Bz-FVR-AMC Related Antibodies

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Description

Bz-FVR-AMC is a fluorogenic substrate for procathepsin with a kcat/Km value of 1070 mM-1s-1. The high concentration of BZ-FVR-AMC inhibits the substrate[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Processing and activation of procathepsin S[2]:
1. Autocatalytic activation of procathepsin S was studied by incubation (final concentration 1-5 μM) at 37 ℃ in 0.5 mL of the appropriate buffer containing 2.5 mM dithiothreitol.
2. Aliquots of 10 μl were taken from the reaction mixture at the times indicated and mixed with 2.5 ml of substrate solution (10 μM Bz-FVR-AMC in 0.1 M phosphate buffer, pH 6.5, containing 1 mM EDTA (HY-Y0682) and 0.1% (w/v) polyethylene glycol 6000).
3. Fluorescence of the released AMC was then monitored continuously for 1 min in a pectrofluorimeter at excitation and emission wavelengths of 370 and 460 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bz-FVR-AMC Related Antibodies
Molecular Weight

681.78

Formula

C37H43N7O6
CAS No.
Unlabeled Cas

Appearance

Solid

Color

White to off-white

Emission (Em)

454
Excitation (Ex)

344
SMILES

CC1=CC(OC2=CC(NC([C@@H](NC([C@H](C(C)C)NC([C@@H](NC(C3=CC=CC=C3)=O)CC4=CC=CC=C4)=O)=O)CCCNC(N)=N)=O)=CC=C12)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (183.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4667 mL 7.3337 mL 14.6675 mL
5 mM 0.2933 mL 1.4667 mL 2.9335 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

V1

=
Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4667 mL 7.3337 mL 14.6675 mL 36.6687 mL
5 mM 0.2933 mL 1.4667 mL 2.9335 mL 7.3337 mL
10 mM 0.1467 mL 0.7334 mL 1.4667 mL 3.6669 mL
15 mM 0.0978 mL 0.4889 mL 0.9778 mL 2.4446 mL
20 mM 0.0733 mL 0.3667 mL 0.7334 mL 1.8334 mL
25 mM 0.0587 mL 0.2933 mL 0.5867 mL 1.4667 mL
30 mM 0.0489 mL 0.2445 mL 0.4889 mL 1.2223 mL
40 mM 0.0367 mL 0.1833 mL 0.3667 mL 0.9167 mL
50 mM 0.0293 mL 0.1467 mL 0.2933 mL 0.7334 mL
60 mM 0.0244 mL 0.1222 mL 0.2445 mL 0.6111 mL
80 mM 0.0183 mL 0.0917 mL 0.1833 mL 0.4584 mL
100 mM 0.0147 mL 0.0733 mL 0.1467 mL 0.3667 mL
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Bz-FVR-AMC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bz-FVR-AMC88899-22-3CathepsinInhibitorinhibitorinhibit

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