2. Metabolic Enzyme/Protease
  3. Phosphodiesterase (PDE)
  4. D159687

D159687 is a selective PDE4D inhibitor.

For research use only. We do not sell to patients.

D159687 Chemical Structure

D159687 Chemical Structure

CAS No. : 1155877-97-6

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

D159687 Related Antibodies

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  • Purity & Documentation

  • References

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Description

D159687 is a selective PDE4D inhibitor[1].

IC50 & Target

PDE4D[1]
In Vitro

D159687 (1 μM, 0-24 hours) induces a transient increase in CREB phosphorylation which peaked at 6 hours after treatment[1].
D159687 (0.01-1 μM, 6 hours) causes optimal CREB phosphorylation at 1 μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

D159687 Related Antibodies

Western Blot Analysis[1]

Cell Line: HT-22 (mouse hippocampal cell line)
Concentration: 1 μM
Incubation Time: 0, 1, 3, 6, 12, 24 hours
Result: Induced a transient increase in CREB phosphorylation which peaked at 6 hours after treatment.

Western Blot Analysis[1]

Cell Line: HT-22 (mouse hippocampal cell line)
Concentration: 0.01 μM, 0.1 μM, 1 μM
Incubation Time: 6 hours
Result: CREB phosphorylation was optimal at 1 μM.
In Vivo

D159687 (0.05-5 mg/kg; oral daily for a week) shows a potential recruitment or enhancement of synaptic function with increased task difficulty in female Cynomolgus macaques[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female Cynomolgus macaques (4–6 year old) [2]
Dosage: 0.05, 0.5, 5 mg/kg
Administration: Oral daily for a week
Result: A potential recruitment or enhancement of synaptic function with increased task difficulty.
Molecular Weight

366.84

Formula

C21H19ClN2O2
CAS No.
Unlabeled Cas

Appearance

Solid

Color

White to off-white

SMILES

NC(NC1=CC=C(CC2=CC(C3=CC(Cl)=CC=C3)=C(OC)C=C2)C=C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 150 mg/mL (408.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7260 mL 13.6299 mL 27.2598 mL
5 mM 0.5452 mL 2.7260 mL 5.4520 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.81 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.81 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7260 mL 13.6299 mL 27.2598 mL 68.1496 mL
5 mM 0.5452 mL 2.7260 mL 5.4520 mL 13.6299 mL
10 mM 0.2726 mL 1.3630 mL 2.7260 mL 6.8150 mL
15 mM 0.1817 mL 0.9087 mL 1.8173 mL 4.5433 mL
20 mM 0.1363 mL 0.6815 mL 1.3630 mL 3.4075 mL
25 mM 0.1090 mL 0.5452 mL 1.0904 mL 2.7260 mL
30 mM 0.0909 mL 0.4543 mL 0.9087 mL 2.2717 mL
40 mM 0.0681 mL 0.3407 mL 0.6815 mL 1.7037 mL
50 mM 0.0545 mL 0.2726 mL 0.5452 mL 1.3630 mL
60 mM 0.0454 mL 0.2272 mL 0.4543 mL 1.1358 mL
80 mM 0.0341 mL 0.1704 mL 0.3407 mL 0.8519 mL
100 mM 0.0273 mL 0.1363 mL 0.2726 mL 0.6815 mL
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D159687 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

D1596871155877-97-6D 159687D-159687Phosphodiesterase (PDE)Inhibitorinhibitorinhibit

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