2. Cell Cycle/DNA Damage
  3. Nucleoside Antimetabolite/Analog
  4. KIN59

KIN59  (Synonyms: 5'-O-Tritylinosine; 5'-O-Trityl-inosine)

Cat. No.: HY-102071 Purity: ≥99.0%
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KIN59 (5’-O-Tritylinosine) is a potent thymidine phosphorylase allosteric inhibitor. KIN59 inhibits FGF2-stimulated cell growth. KIN59 inhibits the expression of p-FGFR1, P-Akt in FGF2 (10 ng/mL) stimulated cells. KIN59 shows anti-tumor activity.

For research use only. We do not sell to patients.

KIN59 Chemical Structure

KIN59 Chemical Structure

CAS No. : 4152-77-6

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5 mg USD 48 In-stock
10 mg USD 77 In-stock
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Based on 1 publication(s) in Google Scholar

KIN59 Related Antibodies

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Description

KIN59 (5’-O-Tritylinosine) is a potent thymidine phosphorylase allosteric inhibitor. KIN59 inhibits FGF2-stimulated cell growth. KIN59 inhibits the expression of p-FGFR1, P-Akt in FGF2 (10 ng/mL) stimulated cells. KIN59 shows anti-tumor activity[1].

In Vitro

KIN59 (0-100 µM; 24 h) inhibits GM7373 cell proliferative with IC50 values of 5.8, 63 µM for FGF2 (30 ng/mL) and PBS (10%) stimulated, respectively[1].
KIN59 (60 µM; 30 min) inhibits the expression of p-FGFR1, P-Akt in FGF2 (10 ng/mL) stimited FGFR1-overexpressing GM7373-FGFR1 cells[1].
KIN59 inhibits recombinant bacterial (E. coli) and human thymidine phosphorylase (TPase) with IC50 values of 44 μM and 67 μM, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

KIN59 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: GM7373 cells
Concentration: 0-100 µM
Incubation Time: 24 h
Result: Inhibited FGF2 (30 ng/mL)-induced proliferation of bovine macrovascular endothelial GM7373 cells in a dose-dependent manner with IC50 values of 5.8, 63 µM for FGF2 and PBS, respectively.

Western Blot Analysis[1]

Cell Line: GM7373-FGFR1, GM7373-VEGFR2 cells
Concentration: 60 µM
Incubation Time: 30 min
Result: Inhibited FGFR1 phosphorylation and Akt activation triggered by FGF2 in FGFR1-overexpressing GM7373-FGFR1 cells, showed minor inhibits on VEGF-mediated VEGFR2 phosphorylation and Akt activation in GM7373 cells overexpressing VEGFR2.
In Vivo

KIN59 (15 mg/kg; s.c.; twice daily from day 2 for 20 days) shows anti-tumor activity in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Eight-week-old female, 25 g, athymic, nude nu/nu mice (F2T-luc2.9 cellss)[1]
Dosage: 15 mg/kg
Administration: S.c.; twice daily from day 2 (once daily during the weekend) at a site distant from the tumor (inoculation) site for 20 days
Result: Caused a significant inhibition in the rate of tumor growth.
Molecular Weight

510.54

Formula

C29H26N4O5
CAS No.
Unlabeled Cas

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](O)[C@H](N2C=NC3=C2N=CN=C3O)O[C@@H]1COC(C4=CC=CC=C4)(C5=CC=CC=C5)C6=CC=CC=C6
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 16 mg/mL (31.34 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9587 mL 9.7936 mL 19.5871 mL
5 mM 0.3917 mL 1.9587 mL 3.9174 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥99.0%

COA (260 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9587 mL 9.7936 mL 19.5871 mL 48.9678 mL
5 mM 0.3917 mL 1.9587 mL 3.9174 mL 9.7936 mL
10 mM 0.1959 mL 0.9794 mL 1.9587 mL 4.8968 mL
15 mM 0.1306 mL 0.6529 mL 1.3058 mL 3.2645 mL
20 mM 0.0979 mL 0.4897 mL 0.9794 mL 2.4484 mL
25 mM 0.0783 mL 0.3917 mL 0.7835 mL 1.9587 mL
30 mM 0.0653 mL 0.3265 mL 0.6529 mL 1.6323 mL
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KIN59 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

KIN594152-77-65'-O-Tritylinosine 5'-O-Trityl-inosineKIN 59KIN-59Nucleoside Antimetabolite/AnalogGM7373 cellsp-FGFR1,P-Aktanti-tumorFGF2Inhibitorinhibitorinhibit

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