2. TGF-beta/Smad
  3. TGF-β Receptor
  4. SB-431542

SB-431542 (GMP) is SB-431542.html" class="link-product" target="_blank"> SB-431542 (HY-10431) produced by using GMP guidelines. GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. SB-431542 is a TGF-β receptor kinase inhibitor (TRKI) in SMAD signaling.

For research use only. We do not sell to patients.

SB-431542 Chemical Structure

SB-431542 Chemical Structure

CAS No. : 301836-41-9

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Customer Review

Based on 183 publication(s) in Google Scholar

Other Forms of SB-431542:

SB-431542 Related Antibodies

Top Publications Citing Use of Products

166 Publications Citing Use of MCE SB-431542

WB
RT-PCR

    SB-431542 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 Mar 16;161:114537.  [Abstract]

    SB431542 (4.2 mg/kg; i.p.; single daily for 3 days) reduces the expression of α-SMA, collagen-I and p-Smad3 in SiO2-induced fibrosis lungs of mice.

    SB-431542 purchased from MedChemExpress. Usage Cited in: Stem Cells. 2019 Feb;37(2):190-201.  [Abstract]

    Western blot images show Smad2/3 phosphorylation is lower than baseline in the sh-GPM6B cells and higher than baseline in the GPM6B-sgRNA cells. The phosphorylation of Smad2/3 in the sh-GPM6B TGF-β1 group is elevated by Alantolactone and the phosphorylation is reduced by treatment with SB431542.

    SB-431542 purchased from MedChemExpress. Usage Cited in: Stem Cells. 2019 Feb;37(2):190-201.  [Abstract]

    Western blot analyses show the repression of SMC specific markers in the sh-GPM6B TGF-β1 group are abolished by Alantolactone in the sh-GPM6B and the enrichment of SMC specific markers in the GPM6B-sgRNA cells is cancelled by treatment with SB431542.

    SB-431542 purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2019 Mar;19(3):2107-2114.  [Abstract]

    Effects of the TGF‑β1 inhibitor SB (20 μM) on mechanical force‑induced COL‑1 and MMP‑1 mRNA expression.

    SB-431542 purchased from MedChemExpress. Usage Cited in: Phytother Res. 2019 Jan;33(1):214-223.  [Abstract]

    Western analysis of FN and α-SMA in cardiac fibroblasts or NIH3T3 with treatments of (+)‐isobicyclogermacrenal, spathulenol, oxymatrine (OMT), and SB431542 at indicated concentrations.

    SB-431542 purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2018 Mar;12(3):305-321.  [Abstract]

    Western blot analysis of EGFR, total and phosphorylated Smad3, total and phosphorylated ERK protein expression in cell lysates from two breast cancer cells pretreated with SB431542 and then stimulated with TGF-β for the indicated times.

    SB-431542 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2018 May 23;19(6). pii: E1556.  [Abstract]

    E-cadherin, N-cadherin, Snail, and MMP-2 expression in HCC cells is examined by Western blot analysis. GAPDH is used as a loading control.

    SB-431542 purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2018 Sep;22(9):4354-4365.  [Abstract]

    p-FOXO3a (Thr32), p-FOXO3a (Ser253), FOXO3a, p-Akt and Akt expression via western blot following treatment with PBS, ASV, TGF-b1, TGF-b1+ASV, TGF-b1+SB431542 or TGF-b1+LY294002.

    SB-431542 purchased from MedChemExpress. Usage Cited in: Immunol Lett. 2019 Feb;206:33-40.  [Abstract]

    Monocytes were cultured with 20 ng/mL M-CSF with or without 10 ng/mL TGF-β1 for 2 days, with SB431542 (1 or 50 μM) or SIS3 (2.5 μM) having been added 30 min beforehand. Whole-cell lysates were subjected to SDS-PAGE and immunoblotted.

    SB-431542 purchased from MedChemExpress. Usage Cited in: Mol Cell Biochem. 2018 Feb;439(1-2):117-129.  [Abstract]

    Protein expression levels of E-cadherin N-cadherin, p-ERK, ERK, TGF-β1, p-Smad2, and p-Smad3 in each group are analyzed by western blotting.

    SB-431542 purchased from MedChemExpress. Usage Cited in: Brain Res Bull. 2018 May;139:38-47.  [Abstract]

    pSmad2/3 protein levels are down-regulated by the ALK5 inhibitor SB431542 (rGDF11 group vs. rGDF11+SB group, p=0.000).

    SB-431542 purchased from MedChemExpress. Usage Cited in: Oncol Lett. 2018 Mar;15(3):2801-2806.  [Abstract]

    A549 cells are co cultured with 10.0 mM SB431542 for 48 h, the expression of TGF β1, Smad2 and caspase 3 are tested by western blot analysis and then analyzed.

    SB-431542 purchased from MedChemExpress. Usage Cited in: Oncol Lett. 2018 Jun;15(6):9681-9686.  [Abstract]

    A549 cells are treated with 1.0% CSE with or without 10.0 mM SB431542 for 48 h, the levels of TGF β1, p Smad2, and MMP3 is assayed by western blot analysis.

    SB-431542 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2017 Apr 18;18(4). pii: E853.  [Abstract]

    The protein level of Six2 and Ki67 after the treatment of phosphorylation Smad3 ( p-Smad3) inhibitor SB431542 (10 µM) for 48 h.

    SB-431542 purchased from MedChemExpress. Usage Cited in: Oncol Lett. 2017 Jul;14(1):541-546.  [Abstract]

    To investigate the effect of SB431542 on the accumulation of TGFβ1 and MMP3 in MM cells, RPMI 8226 cells are treated with 10, 100 and 1,000 nmol/mL SB431542 for 48 h. Western blotting is performed. The results demonstrate that SB431542 significantly decreases the accumulation of TGFβ1 and MMP3 at 100 and 1,000 nmol/mL when compared with the control. Smad2, as a signaling molecule, can be initially activated by TGFβ1. Therefore, the effect of SB431542 on the expression of Smad2 and p-Smad2 in RPM

    SB-431542 purchased from MedChemExpress. Usage Cited in: Chin J Immun. 2016 (5): 615-619.

    10-1000 nM of SB431542 are co-cultured with RLE-6TN cells for 48 h. Expressions of p-Smad2 and Smad are detected by Western blot.

    SB-431542 purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2015 Sep;28(1):707-14.  [Abstract]

    SB431542 is used to block the TGF-β1/Smad2 pathway and the expression of PLTP, p-Smad2 and cleaved caspase-3 is analyzed. (A) RLE-6TN cells are treated with 5–500 nM SB431542 for 72 h. Expression of PLTP and p-Smad2 is analyzed by Western blot. (B) CSE-treated rat lung epithelial cells are treated with 500 nM SB431542 to block the TGF-β1/Smad2 pathway.

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    Description

    SB-431542 (GMP) is SB-431542.html" class="link-product" target="_blank"> SB-431542 (HY-10431) produced by using GMP guidelines. GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. SB-431542 is a TGF-β receptor kinase inhibitor (TRKI) in SMAD signaling[1][2][3].

    IC50 & Target[1]

    ALK4

    1 μM (IC50)

    ALK5

    0.75 μM (IC50)

    ALK7

    2 μM (IC50)

    In Vitro

    SB-431542 (GMP) (10 μM; 10 days) induces human pluripotent stem cells (hPSCs) to post-mitotic cortical neurons differentiation[1].
    SB-431542 (GMP) (10 μM) facilitates directed differentiation into midbrain dopamine neurons (mDA)[2].
    SB-431542 (GMP) (3.8 μM) promotes differentiation of hPSCs to multipotent hematopoietic progenitors that arise from SOX17+ hemogenic endothelium in vitro[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    SB-431542 Related Antibodies

    RT-PCR[1]

    Cell Line: Human pluripotent stem cells (hPSCs)
    Concentration: 10 μM
    Incubation Time: 10 days
    Result: Showed downregulation of the pluripotency marker OCT4 and induction of neural and neuronal markers PAX6, FOXG1 and DCX, as well as markers of early born cortical neurons, including TBR1 (preplate, subplate and layer VI) and REELIN, in LSB+X/P/S/D conditions.
    Molecular Weight

    384.39

    Formula

    C22H16N4O3
    CAS No.
    Unlabeled CAS

    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(C1=CC=C(C=C1)C2=NC(C3=CC=C4OCOC4=C3)=C(N2)C5=NC=CC=C5)N
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
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    SB-431542 Related Classifications

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    SB-431542 (GMP)301836-41-9TGF-β ReceptorTransforming growth factor beta receptorsmidbrain dopamine (mDA)neuronSMADhuman pluripotent stem cells (hPSCs)Inhibitorinhibitorinhibit

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