2. Cell Cycle/DNA Damage Metabolic Enzyme/Protease
  3. DNA/RNA Synthesis Endogenous Metabolite
  4. Thymidine-d4

Thymidine-d4  (Synonyms: DThyd-d4; NSC 21548-d4)

Cat. No.: HY-N1150S1 Purity: ≥99.0%
COA Handling Instructions

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Thymidine-d4 is the deuterium labeled Thymidine. Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication[1][2][3].

For research use only. We do not sell to patients.

Thymidine-d<sub>4</sub> Chemical Structure

Thymidine-d4 Chemical Structure

CAS No. : 347841-67-2

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1 mg USD 380 In-stock
5 mg USD 1100 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Thymidine-d4:

Thymidine-d4 Related Antibodies

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Description

Thymidine-d4 is the deuterium labeled Thymidine. Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication[1][2][3].

In Vitro

Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Thymidine-d4 Related Antibodies
Molecular Weight

246.25

Formula

C10H10D4N2O5
CAS No.
Unlabeled Cas

50-89-5
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1C[C@H](N2C([2H])=C(C([2H])([2H])[2H])C(NC2=O)=O)O[C@@H]1CO
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMF : ≥ 16 mg/mL (64.97 mM)

DMSO : ≥ 10 mg/mL (40.61 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

PBS (pH 7.2) : ≥ 5 mg/mL (20.30 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0609 mL 20.3046 mL 40.6091 mL
5 mM 0.8122 mL 4.0609 mL 8.1218 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥99.0%

COA (257 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
PBS (pH 7.2) / DMSO / DMF 1 mM 4.0609 mL 20.3046 mL 40.6091 mL 101.5228 mL
5 mM 0.8122 mL 4.0609 mL 8.1218 mL 20.3046 mL
10 mM 0.4061 mL 2.0305 mL 4.0609 mL 10.1523 mL
15 mM 0.2707 mL 1.3536 mL 2.7073 mL 6.7682 mL
20 mM 0.2030 mL 1.0152 mL 2.0305 mL 5.0761 mL
DMSO / DMF 25 mM 0.1624 mL 0.8122 mL 1.6244 mL 4.0609 mL
30 mM 0.1354 mL 0.6768 mL 1.3536 mL 3.3841 mL
40 mM 0.1015 mL 0.5076 mL 1.0152 mL 2.5381 mL
DMF 50 mM 0.0812 mL 0.4061 mL 0.8122 mL 2.0305 mL
60 mM 0.0677 mL 0.3384 mL 0.6768 mL 1.6920 mL
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Thymidine-d4 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Thymidine-d4347841-67-2DThyd-d4NSC 21548-d4DNA/RNA SynthesisEndogenous MetaboliteInhibitorinhibitorinhibit

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