2. Cell Cycle/DNA Damage Cytoskeleton Apoptosis
  3. Microtubule/Tubulin Ferroptosis
  4. Tubulin inhibitor 30

Tubulin inhibitor 30 (Compound 15) is a tubulin assembly inhibitor with an IC50 of 0.52 μM. Tubulin inhibitor 30 can induce ferroptosis.

For research use only. We do not sell to patients.

Tubulin inhibitor 30 Chemical Structure

Tubulin inhibitor 30 Chemical Structure

CAS No. : 2873383-67-4

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Based on 1 publication(s) in Google Scholar

Tubulin inhibitor 30 Related Antibodies

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Description

Tubulin inhibitor 30 (Compound 15) is a tubulin assembly inhibitor with an IC50 of 0.52 μM. Tubulin inhibitor 30 can induce ferroptosis[1].

IC50 & Target

IC50: 0.52 μM (Tubulin)[1]
In Vitro

Tubulin inhibitor 30 (Compound 15) (0-100 nM; 48 h) inhibits the viability of MCF-7, U-87 MG and OVCAR-3 cells[1].
Tubulin inhibitor 30 is metabolically stable when incubated with human liver microsomes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tubulin inhibitor 30 Related Antibodies

Cell Viability Assay[1]

Cell Line: MCF-7, U-87 MG and OVCAR-3 cells
Concentration: 0-100 nM
Incubation Time: 48 h
Result: Inhibited the cell viability with IC50s of 4.0, 10.06 and 2.852 nM against MCF-7, U-87 MG and OVCAR-3 cells, respectively.

Western Blot Analysis[1]

Cell Line: U-87 MG and OVCAR-3 cells
Concentration: 0-100 nM
Incubation Time: 48 h
Result: Induced a significant increase of cleaved PARP.
In Vivo

Tubulin inhibitor 30 (Compound 15) (25mg/kg; i.p.; every 2 days) inhibits the in vivo tumorigenicity of the human GBM cell line U-87 MG and the human OC cell line SKOV-3, and causes oxidative stress injury and Fe2+ accumulation in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/Cnu/nu mice, U-87 MG or SKOV-3 model[1]
Dosage: 25 mg/kg
Administration: Intraperitoneal injection, every 2 days
Result: Inhibited tumorigenicity and tumor volume. Caused oxidative stress injury and Fe2+ accumulation.
Molecular Weight

405.40

Formula

C22H19N3O5
CAS No.
Unlabeled CAS

Appearance

Solid

Color

White to off-white

SMILES

COC1=CC(C(C2=CN(C=C2C3=CC=CO3)C4=NC=CC=N4)=O)=CC(OC)=C1OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (12.33 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4667 mL 12.3335 mL 24.6670 mL
5 mM 0.4933 mL 2.4667 mL 4.9334 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 0.5 mg/mL (1.23 mM); Clear solution

    This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4667 mL 12.3335 mL 24.6670 mL 61.6675 mL
5 mM 0.4933 mL 2.4667 mL 4.9334 mL 12.3335 mL
10 mM 0.2467 mL 1.2333 mL 2.4667 mL 6.1667 mL
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Tubulin inhibitor 30 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tubulin inhibitor 302873383-67-4Tubulin inhibitor30Tubulin inhibitor-30Microtubule/TubulinFerroptosisMCF-7U-87 MGOVCAR-3metabolically stableInhibitorinhibitorinhibit

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