1alpha-Hydroxy VD4 (Synonyms: 1α-Hydroxy vitamin D4)

Cat. No.: HY-13249 Purity: 98.08%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

1alpha-Hydroxy VD4 , a 1alpha(OH)D derivative, can effectively induce the differentiation of monoblastic leukaemia U937, P39/TSU and P31/FUJ cells.

For research use only. We do not sell to patients.

1alpha-Hydroxy VD4 Chemical Structure

CAS No. : 143032-85-3

Size	Price	Stock	Quantity
1 mg	USD 1056	In-stock	Estimated Time of Arrival: December 31
5 mg		Get quote
10 mg		Get quote

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Description

1alpha-Hydroxy VD4 , a 1alpha(OH)D derivative, can effectively induce the differentiation of monoblastic leukaemia U937, P39/TSU and P31/FUJ cells. IC50 value； Target: VD analog

Molecular Weight

414.66

Formula

C₂₈H₄₆O₂

CAS No.

143032-85-3

Appearance

Solid

Color

White to off-white

SMILES

C=C1[C@@H](O)C[C@H](O)C/C1=C/C=C2[C@@](CC[C@@H]3[C@H](C)CC[C@H](C)C(C)C)([H])[C@]3(C)CCC/2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (120.58 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4116 mL	12.0581 mL	24.1161 mL
5 mM	0.4823 mL	2.4116 mL	4.8232 mL
10 mM	0.2412 mL	1.2058 mL	2.4116 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.41%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (252 KB) HNMR (235 KB) RP-HPLC (240 KB)

Handling Instructions (2659 KB)

References

[1]. M Makishima, et al. Growth inhibition and differentiation induction in human monoblastic leukaemia cells by 1alpha-hydroxyvitamin D derivatives and their enhancement by combination with hydroxyurea. British Journal of Cancer (1998) 77, 33-39

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4116 mL	12.0581 mL	24.1161 mL	60.2904 mL
	5 mM	0.4823 mL	2.4116 mL	4.8232 mL	12.0581 mL
	10 mM	0.2412 mL	1.2058 mL	2.4116 mL	6.0290 mL
	15 mM	0.1608 mL	0.8039 mL	1.6077 mL	4.0194 mL
	20 mM	0.1206 mL	0.6029 mL	1.2058 mL	3.0145 mL
	25 mM	0.0965 mL	0.4823 mL	0.9646 mL	2.4116 mL
	30 mM	0.0804 mL	0.4019 mL	0.8039 mL	2.0097 mL
	40 mM	0.0603 mL	0.3015 mL	0.6029 mL	1.5073 mL
	50 mM	0.0482 mL	0.2412 mL	0.4823 mL	1.2058 mL
	60 mM	0.0402 mL	0.2010 mL	0.4019 mL	1.0048 mL
	80 mM	0.0301 mL	0.1507 mL	0.3015 mL	0.7536 mL
	100 mM	0.0241 mL	0.1206 mL	0.2412 mL	0.6029 mL

1alpha-Hydroxy VD4 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

1alpha-Hydroxy VD4143032-85-31α-Hydroxy vitamin D4VD/VDRVitamin DVitamin D receptorInhibitorinhibitorinhibit

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