2. Metabolic Enzyme/Protease Apoptosis Autophagy
  3. Hexokinase Apoptosis Autophagy
  4. 3-Bromopyruvic acid

3-Bromopyruvic acid  (Synonyms: Bromopyruvic acid; Hexokinase II Inhibitor II, 3-BP)

Cat. No.: HY-19992 Purity: 98.00%
COA Handling Instructions

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3-Bromopyruvate (Bromopyruvic acid) is an analogue of pyruvate and a potent hexokinase (HK)-II inhibitor with high tumor selectivity. 3-Bromopyruvate inhibits cell growth and induces apoptosis through interfering with glycolysis. 3-Bromopyruvate induces autophagy by stimulating ROS formation in breast cancer cells. Antimicrobial activities.

For research use only. We do not sell to patients.

3-Bromopyruvic acid Chemical Structure

3-Bromopyruvic acid Chemical Structure

CAS No. : 1113-59-3

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10 mM * 1 mL in DMSO
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Based on 17 publication(s) in Google Scholar

3-Bromopyruvic acid Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

3-Bromopyruvate (Bromopyruvic acid) is an analogue of pyruvate and a potent hexokinase (HK)-II inhibitor with high tumor selectivity. 3-Bromopyruvate inhibits cell growth and induces apoptosis through interfering with glycolysis. 3-Bromopyruvate induces autophagy by stimulating ROS formation in breast cancer cells. Antimicrobial activities[1][2][3].

In Vitro

3-Bromopyruvate enhances TRAIL-induced apoptosis in breast cancer cells[2].
3-Bromopyruvate (Bromopyruvic acid), a hexokinase II inhibitor, can induce apoptosis in hepatocellular carcinoma cells by inducing endoplasmic reticulum (ER) stress[2].
3-Bromopyruvate inhibits ATP generation and upregulates the expression of DR5. 3-Bromopyruvate upregulates CHOP, GRP78 and the phosphorylation of AMPK and augments TRAIL-induced Bax and caspase-3 levels[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3-Bromopyruvic acid Related Antibodies

Cell Viability Assay[2]

Cell Line: MCF-7 and MDA-MB-231 cells
Concentration: 40, 80, 160 or 320 µM
Incubation Time: 24 hours
Result: 3-Bromopyruvate (80 and 160 µmol/l) and TRAIL (400 ng/ml) significantly inhibited cell viability.
In Vivo

3-Bromopyruvate (8 mg/kg; i.p.; every 4 days for 28 days) shows a synergistic antitumor effect in MCF-7 cell xenografts in nude mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female nude mice (BALB/c; 4-5-weeks old and 18-20 g)[2]
Dosage: 8 mg/kg
Administration: I.p.; every 4 days for 28 days
Result: Showed antitumor efficacy in tumor xenografts.
Molecular Weight

166.96

Formula

C3H3BrO3
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C(O)C(CBr)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 250 mg/mL (1497.36 mM; Need ultrasonic)

DMSO : 100 mg/mL (598.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.9895 mL 29.9473 mL 59.8946 mL
5 mM 1.1979 mL 5.9895 mL 11.9789 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (14.97 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (14.97 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (598.95 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

COA (245 KB) HNMR (171 KB) MS (74 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 5.9895 mL 29.9473 mL 59.8946 mL 149.7365 mL
5 mM 1.1979 mL 5.9895 mL 11.9789 mL 29.9473 mL
10 mM 0.5989 mL 2.9947 mL 5.9895 mL 14.9736 mL
15 mM 0.3993 mL 1.9965 mL 3.9930 mL 9.9824 mL
20 mM 0.2995 mL 1.4974 mL 2.9947 mL 7.4868 mL
25 mM 0.2396 mL 1.1979 mL 2.3958 mL 5.9895 mL
30 mM 0.1996 mL 0.9982 mL 1.9965 mL 4.9912 mL
40 mM 0.1497 mL 0.7487 mL 1.4974 mL 3.7434 mL
50 mM 0.1198 mL 0.5989 mL 1.1979 mL 2.9947 mL
60 mM 0.0998 mL 0.4991 mL 0.9982 mL 2.4956 mL
80 mM 0.0749 mL 0.3743 mL 0.7487 mL 1.8717 mL
100 mM 0.0599 mL 0.2995 mL 0.5989 mL 1.4974 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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3-Bromopyruvic acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3-Bromopyruvic acid1113-59-3Bromopyruvic acid Hexokinase II Inhibitor II, 3-BPHexokinaseApoptosisAutophagyanaloguepyruvateglycolysisERstressMCF-7MDA-MB-231cellsInhibitorinhibitorinhibit

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