3-Methyladenine (Synonyms: 3-MA)

Name: 3-Methyladenine
Brand: MedChemExpress
SKU: HY-19312
Rating: 5.0 (685 reviews)

Cat. No.: HY-19312 Purity: 99.91%: FAQs
Data Sheet SDS COA Handling Instructions

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3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K.

For research use only. We do not sell to patients.

3-Methyladenine Chemical Structure

CAS No. : 5142-23-4

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50 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 96	In-stock	Estimated Time of Arrival: December 31
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Based on 685 publication(s) in Google Scholar

Other Forms of 3-Methyladenine:

3-Methyladenine-d₃ In-stock

663 Publications Citing Use of MCE 3-Methyladenine

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Feb 28;21(1):69. [Abstract]; 3-Methyladenine (3-MA; 5 mM; 24 h) markedly decreases the SWCNTs-induced increase in LC3B puncta, in MLE-12 cells.

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Feb 28;21(1):69. [Abstract]; The autophagy is inhibited by 3-Methyladenine (3-MA; 5 mM; 24 h), as evidenced by decreased Atg5 expression and the ratio of LC3-II/LC3-I and increased p62 protein level in MLE-12 cells (Fig. B, C).

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: Brain Behav Immun. 2019 Jul;79:244-255. [Abstract]; Immunoblot analysis of NLRP3 in LPS-primed macrophages treated with KA (10 μM) or concurrently with autophagy inhibitor (3-MA, 5 mM). Statistical analysis of NLRP3 intensity during 3-MA or zaprinast treatment is shown.

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: Front Endocrinol (Lausanne). 2019 Jun 17;10:370. [Abstract]; LC3 II expression is regulated in CQ- or 3-MA-treated GnRH-OE Panc1 cells.

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2019 Apr 15;170:112-119. [Abstract]; Western blotting image protein levels of p62 in DF-1 cells treated with different supplements (+ represent supplying, - represents not supplying a certain amount of Cr(VI), 4-PBA, Tg, NS-398, 3-MA and Rapamycin).

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2019 Apr 15;170:112-119. [Abstract]; Western blotting image protein levels of GRP78/Bip in DF-1 cells treated with different supplements (+ represent supplying, - represents not supplying a certain amount of Cr(VI), 4-PBA, Tg, NS-398, 3-MA and Rapamycin).

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2019 Apr 15;170:112-119. [Abstract]; Western blotting image protein levels of LC3-I & LC3-II in DF-1 cells treated with different supplements (+ represent supplying, - represents not supplying a certain amount of Cr(VI), 4-PBA, Tg, NS-398, 3-MA and Rapamycin).

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: FASEB J. 2019 Apr;33(4):5520-5534. [Abstract]; CRC cells are treated with vehicle, BFA (100 nM), 3-MA (10 mM), or in combination for 24 h. Immunoblot analysis is used to detect LC3 turnover.

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: J Cell Biochem. 2019 Mar;120(3):4366-4374. [Abstract]; Representative Western blots show LC3 I/II and p62 protein expression in kidneys of vehicle group and 3-MA group mice at 72 hours after the CDDP treatment.

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2018 May;41(5):2535-2544. [Abstract]; MC3T3-E1 cells are treated with AICAR (10 μM) in the presence or absence of 3-MA (5 mM) or CQ (10 μM) for 24 h, after which western blot analysis is conducted.

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2018 Jun;41(6):3221-3230. [Abstract]; Western blot analysis is used to measure LC3, p62 and Beclin‑1 expression following the treatment of H9C2 cells with si‑miRNA‑30e and 3‑MA.

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2018 Oct;22(10):5132-5144. [Abstract]; Melatonin regulation of mitophagy activity in the diabetic myocardium, Protein expression with representative gel blots of LC3 I/II, GAPDH (loading control).

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: Int J Oncol. 2018 Sep;53(3):1363-1373. [Abstract]; The protein expression levels of LC3 and Beclin-1 proteins in HCT-116 cells. Cells are treated with 100 μg/mL active fraction of clove (AFC) for 48 h with or without pretreatment of 2 mM 3-MA or 1 nM Baflomycin A1 (BA) for 1 h.

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: J Dermatol Sci. 2018 Jan;89(1):11-18. [Abstract]; Inhibitory effects of LED 585 nm on melanogenesis is reversed by 3-MA.

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: J Dermatol Sci. 2018 Jan;89(1):11-18. [Abstract]; Inhibitory effects of LED 585 nm on melanogenesis is reversed by 3-MA. Autophagy is decreased in 3-MA-treated group, which is reflected by the decrease in the ratio of LC3 II/LC3 I and increase in p62 protein

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: J Immunol Res. 2018 Dec 24;2018:9807139. [Abstract]; Western blot assays show that 3-MA abrogates the Alda-1 induced expression increases in LC3BⅡ and Bcl2 and decreases in P62 and Bax.

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2018 Feb 1;50(2):144-155. [Abstract]; Autophagy is involved in Rg1-inhibited apoptosis in macrophages Macrophages with serum deprivation are treated with 50 μM Rg1 for 48 h in the absence or presence of 3-MA (5 mM). The protein expression level of cleaved caspase-3 determined by western blot analysis in Raw264.7 macrophages.

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: PLoS One. 2018 Aug 6;13(8):e0201920. [Abstract]; The pretreatment of Z-VAD-FMK significantly reduces the levels of cleaved caspase-3 and cleaved PARP. The pretreatment of 3-MA blocks the conversion of LC3-I to LC3-II.

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: Biomed Res. 2018;39(2):87-94. [Abstract]; Accumulation of LC3B II and p62 proteins in the livers of chloroform-treated mice is reduced by 3-MA treatment.

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: Cell Physiol Biochem. 2018;48(6):2318-2336. [Abstract]; Cells are pretreated with 5 mM 3-MA or 2 μM for 1 h and then exposed to 40 μM EPA and 10 μM Rp for 48 h. Cell extracts are prepared and subjected to western blot analysis.

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: Oncotarget. 2017 Nov 22;8(65):109135-109150. [Abstract]; Cell morphology of MCF-7 treated with RA (20 μM)+ω-3 PUFAs (80 μM) with or without 3-MA (5 mM) for 24h.

: 3-Methyladenine purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2016 Dec 2;481(1-2):90-96. [Abstract]; The hepatic stellate cells (HSCs) activation is down-regulated along with autophagy following DMKG or 3MA treatment in vitro. Western blot analysis of LC3B, Beclin-1, α-SMA, and collagen-I expression from lysates of HSC-T6 incubated with 4mM DMKG or 10 mM 3MA for 12 h.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K^[1].

IC₅₀ & Target^[1]

PtdIns3Kγ

60 μM (IC₅₀, Cell Assay)

Vps34

25 μM (IC₅₀, Cell Assay)

Autophagy

Mitophagy

Human Endogenous Metabolite

In Vitro

3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner^[2].
3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions^[2].
3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy^[2].
3-Methyladenine induces cell death is independent of autophagy inhibition^[2].
3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest^[2].
Note:
The recommended concentration of 3-MA is approximately 0.5-10 mM in cell culture. DMSO stock solution is not recommended. We suggest that you can weigh out the amount of 3-MA you required before your experiment. Then please dissolve it in medium and sterilize with a 0.22 μm filter.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	HeLa cells
Concentration:	0 mM, 2.5 mM or 5 mM, 10 mM
Incubation Time:	0 hour, 24 hours and 48 hours
Result:	Decreased cell viability in a time-and dose-dependent manner, and was associated with caspase-3 activation.

Cell Autophagy Assay^[2]

Cell Line:	HeLa cells
Concentration:	5 mM
Incubation Time:	24 hours
Result:	Suppressed autophagy in HeLa cells under both glucose-free conditions and normal conditions.

Western Blot Analysis^[2]

Cell Line:	HeLa cells
Concentration:	5 mM
Incubation Time:	0 hour, 12 hours, 24 hours and 48 hours
Result:	Suppressed conversion of LC3-I to LC3-II between 12 hours and 48 hours.

In Vivo

3-Methyladenine (1.5 mg/100 g; intraperitoneal injection; 3-24 hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24 hours^[3].
3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP^[3].
3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	10–12 weeks Specific pathogen free- (SPF-) grade healthy male Sprague-Dawley (SD) rats (250–290 g)^[3]
Dosage:	1.5 mg/100 g (1000 μM)
Administration:	Intraperitoneal injection
Result:	Alleviated Sodium Taurocholate-Induced SAP.

Molecular Weight

149.15

Formula

C₆H₇N₅

CAS No.

5142-23-4

Appearance

Solid

Color

White to off-white

SMILES

NC1=C2N=CN=C2N(C)C=N1

Structure Classification

Others

Initial Source

Microorganisms

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 5 mg/mL (33.52 mM; Need ultrasonic)

DMSO : 8.33 mg/mL (55.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.7047 mL	33.5233 mL	67.0466 mL
5 mM	1.3409 mL	6.7047 mL	13.4093 mL
10 mM	0.6705 mL	3.3523 mL	6.7047 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 4 mg/mL (26.82 mM); Clear solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 25 mg/mL (167.62 mM); Clear solution; Need ultrasonic and warming and heat to 50°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (282 KB) SDS (393 KB)

COA (251 KB) HNMR (200 KB) LCMS (257 KB) Elemental Analysis Report (110 KB)

Handling Instructions (2659 KB)

References

[1]. Miller S, et al. Finding a fitting shoe for Cinderella: searching for an autophagy inhibitor. Autophagy. 2010 Aug;6(6):805-7. [Content Brief]

[2]. Hou H, et al. Inhibitors of phosphatidylinositol 3'-kinases promote mitotic cell death in HeLa cells. PLoS One. 2012;7(4):e35665. [Content Brief]

[3]. Wang X, et al. Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats.Mediators Inflamm. 2016;2016:8369704. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	6.7047 mL	33.5233 mL	67.0466 mL	167.6165 mL
	5 mM	1.3409 mL	6.7047 mL	13.4093 mL	33.5233 mL
	10 mM	0.6705 mL	3.3523 mL	6.7047 mL	16.7617 mL
	15 mM	0.4470 mL	2.2349 mL	4.4698 mL	11.1744 mL
	20 mM	0.3352 mL	1.6762 mL	3.3523 mL	8.3808 mL
	25 mM	0.2682 mL	1.3409 mL	2.6819 mL	6.7047 mL
	30 mM	0.2235 mL	1.1174 mL	2.2349 mL	5.5872 mL
DMSO	40 mM	0.1676 mL	0.8381 mL	1.6762 mL	4.1904 mL
DMSO	50 mM	0.1341 mL	0.6705 mL	1.3409 mL	3.3523 mL

3-Methyladenine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3-Methyladenine5142-23-43-MAPI3KAutophagyMitophagyEndogenous MetabolitePhosphoinositide 3-kinaseMitochondrial AutophagyInhibitorinhibitorinhibit

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3-Methyladenine (Synonyms: 3-MA)

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Other Forms of 3-Methyladenine:

3-Methyladenine Related Antibodies

663 Publications Citing Use of MCE 3-Methyladenine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All PI3K Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

3-Methyladenine Related Antibodies

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Complete Stock Solution Preparation Table

3-Methyladenine Related Classifications

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