1. Apoptosis PI3K/Akt/mTOR Stem Cell/Wnt MAPK/ERK Pathway
  2. Apoptosis PI3K Akt ERK
  3. 4-Methylbenzylidene camphor

4-Methylbenzylidene camphor  (Synonyms: 4-MBC; Enzacamene)

Cat. No.: HY-17587 Purity: 99.87%
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4-Methylbenzylidene camphor (4-MBC) is an endocrine disrupter that produces estrogen-like effects. 4-Methylbenzylidene camphor decreases the proliferation of human trophoblast cells and induces apoptosis. 4-Methylbenzylidene camphor activates PI3K/AKT and ERK1/2 signaling pathways and elevates intracellular ROS production. 4-Methylbenzylidene camphor is a ultraviolet (UV) filter and may hamper normal placental formation during early pregnancy.

For research use only. We do not sell to patients.

4-Methylbenzylidene camphor Chemical Structure

4-Methylbenzylidene camphor Chemical Structure

CAS No. : 36861-47-9

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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of 4-Methylbenzylidene camphor:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 4-Methylbenzylidene camphor

IHC
WB
Proliferation Assay

    4-Methylbenzylidene camphor purchased from MedChemExpress. Usage Cited in: Reprod Toxicol. 2019 Mar;84:49-58.  [Abstract]

    The effects of 4-MBC (50 μM) alone or with selective kinase inhibitors on proliferation of HTR8/SVneo cells. LY294002 (AKT inhibitor, 20 μM) and U0126 (ERK1/2 inhibitor, 20 μM) are co-treated with 4-MBC for 48 h in triplicate.

    4-Methylbenzylidene camphor purchased from MedChemExpress. Usage Cited in: Reprod Toxicol. 2019 Mar;84:49-58.  [Abstract]

    4-Methylbenzylidene-camphor (4-MBC) dose-dependently affects phosphorylation of protein kinases in HTR8/SVneo cells. Concentration-dependent phosphorylation of AKT, P70S6K, S6, GSK3β, ERK1/2, and P90RSK in response to 4-MBC for 2 h is estimated. Immunoblots are imaged to calculate normalized values by estimating the abundance of phosphorylated proteins relative to total proteins.

    4-Methylbenzylidene camphor purchased from MedChemExpress. Usage Cited in: Reprod Toxicol. 2019 Mar;84:49-58.  [Abstract]

    4-Methylbenzylidene-camphor (4-MBC) time-dependently affects phosphorylation of protein kinases in HTR8/SVneo cells. Time-dependent phosphorylation of AKT, P70S6K, S6, GSK3β, ERK1/2, and P90RSK in response to 50 μM 4-MBC is estimated. Immunoblots are imaged to calculate normalized values by estimating the abundance of phosphorylated proteins relative to total proteins.

    4-Methylbenzylidene camphor purchased from MedChemExpress. Usage Cited in: Reprod Toxicol. 2019 Mar;84:49-58.  [Abstract]

    PCNA protein is detected (green) and nuclei are counterstained with 4′,6-diamidino-2-phenylindole (DAPI, blue) in HTR8/SVneo cells treated with 50 μM 4-MBC for 24 h.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    4-Methylbenzylidene camphor (4-MBC) is an endocrine disrupter that produces estrogen-like effects. 4-Methylbenzylidene camphor decreases the proliferation of human trophoblast cells and induces apoptosis. 4-Methylbenzylidene camphor activates PI3K/AKT and ERK1/2 signaling pathways and elevates intracellular ROS production. 4-Methylbenzylidene camphor is a ultraviolet (UV) filter and may hamper normal placental formation during early pregnancy[1][2].

    IC50 & Target[1]

    PI3K

     

    Akt

     

    ERK1

     

    ERK2

     

    In Vitro

    4-Methylbenzylidene camphor (4-MBC; 5-400 μM; for 48 h) inhibits proliferation of HTR8/SVneo cell[1].
    4-Methylbenzylidene camphor (10-50 μM; for 48 h) induces apoptotic cell death of human trophoblast cells[1].
    4-Methylbenzylidene camphor (5-50 μM; for 48 h) increased the proportion of cells in the SubG1 phase[1].
    4-Methylbenzylidene camphor (50 μM; for 48 h) reduces invasion of human trophoblast cells[1].
    4-Methylbenzylidene camphor (50 μM; 5-120 min) activates PI3K/AKT and ERK1/2 signaling pathways in human trophoblast cells[1].
    4-Methylbenzylidene camphor (20-50 μM; 24 h) significantly increases the expression of SEMA6 A, GPR56, ITGB4, EPHB4, NRP1[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Proliferation Assay[1]

    Cell Line: HTR8/SVneo cells
    Concentration: 0, 5, 10, 20, 50, 100, 200, and 400 μM
    Incubation Time: 48 h
    Result: Dose-dependently inhibited cell proliferation of HTR8/SVneo cell.

    Apoptosis Analysis[1]

    Cell Line: HTR8/SVneo cells
    Concentration: 10, 20, 50 μM
    Incubation Time: 48 h
    Result: Early and late apoptotic cells was significantly increased at 20 μM and 50 μM.

    Cell Cycle Analysis[1]

    Cell Line: HTR8/SVneo cells
    Concentration: 5, 10, 20, 50 μM
    Incubation Time: 48 h
    Result: Gradually increased the proportion of cells in the SubG1 phase.

    Cell Invasion Assay[1]

    Cell Line: HTR8/SVneo cells
    Concentration: 50 μM
    Incubation Time: 48 h
    Result: Revealed a significant reduction of 81.5% in invasiveness

    Western Blot Analysis[1]

    Cell Line: HTR8/SVneo cells
    Concentration: 50 μM
    Incubation Time: 0, 5, 15, 30, 60, 120 min
    Result: The phosphorylation of AKT and its downstream kinase protein, P70S6K, peaked at 5 and 15 min, respectively, subsequently decreased after 30 min, and then reactivated at 120 min

    RT-PCR[1]

    Cell Line: HTR8/SVneo cells
    Concentration: 20, 50 μM
    Incubation Time: 24 h
    Result: Significantly increased the expression of semaphorin 6 A (SEMA6 A), GPR56, integrin subunit beta 4 (ITGB4), EPHB4, neuropilin 1 (NRP1).
    In Vivo

    4-Methylbenzylidene camphor (4-MBC; 0.7, 7, 24, 47 mg/kg/day; administered in chow to the parent generation before mating, during pregnancy and lactation, and to the offspring until adulthood) causes exhibited enhanced prostate growth and altered uterine gene expression in Neonates[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    254.37

    Formula

    C18H22O

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CC1(C)[C@@H]2CC[C@]1(C)C(/C2=C\C3=CC=C(C)C=C3)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (393.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.9313 mL 19.6564 mL 39.3128 mL
    5 mM 0.7863 mL 3.9313 mL 7.8626 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (9.83 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (9.83 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Dosing volume
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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.87%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.9313 mL 19.6564 mL 39.3128 mL 98.2820 mL
    5 mM 0.7863 mL 3.9313 mL 7.8626 mL 19.6564 mL
    10 mM 0.3931 mL 1.9656 mL 3.9313 mL 9.8282 mL
    15 mM 0.2621 mL 1.3104 mL 2.6209 mL 6.5521 mL
    20 mM 0.1966 mL 0.9828 mL 1.9656 mL 4.9141 mL
    25 mM 0.1573 mL 0.7863 mL 1.5725 mL 3.9313 mL
    30 mM 0.1310 mL 0.6552 mL 1.3104 mL 3.2761 mL
    40 mM 0.0983 mL 0.4914 mL 0.9828 mL 2.4571 mL
    50 mM 0.0786 mL 0.3931 mL 0.7863 mL 1.9656 mL
    60 mM 0.0655 mL 0.3276 mL 0.6552 mL 1.6380 mL
    80 mM 0.0491 mL 0.2457 mL 0.4914 mL 1.2285 mL
    100 mM 0.0393 mL 0.1966 mL 0.3931 mL 0.9828 mL
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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