2. Metabolic Enzyme/Protease
  3. Phosphatase
  4. 4-Methylumbelliferyl phosphate

4-Methylumbelliferyl phosphate  (Synonyms: 4-MUP; MUP)

Cat. No.: HY-D0994 Purity: 99.96%
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4-Methylumbelliferyl phosphate (4-MUP), an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate is also a nerve agent simulant.

For research use only. We do not sell to patients.

4-Methylumbelliferyl phosphate Chemical Structure

4-Methylumbelliferyl phosphate Chemical Structure

CAS No. : 3368-04-5

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in Water USD 55 In-stock
Solid
100 mg USD 50 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of 4-Methylumbelliferyl phosphate:

4-Methylumbelliferyl phosphate Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

4-Methylumbelliferyl phosphate (4-MUP), an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate is also a nerve agent simulant[1][2][3].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Serum acid phosphatase assay[1]:
1. Preparation a 100 μL solution containing the following agents: 5.0 μL of serum enzyme, 50 μL of 4-Methylumbelliferyl phosphate (5.0 mM), and 10 μL of 1.0 M buffer at pH 6.0.
2. Add the following agents (with the final concentration) 20 mM Sodium Tartrate (HY-128476), 15 mM Sodium Fluoride (HY-B1766), 3000 mM 2-Mercaptoethanol, 4 mM 1, 10-Phenanthroline (HY-W004544), 6 mM 8-Quinolinol (HY-B1005), 6 mM 8-Hydroxy-5quinolinesulfonic acid, 45 mM EDTA (HY-Y0682) and 6 mM EGTA (HY-D0861).
3. Incubate reaction mixtures for 15 min at 37℃ and terminated by the addition of 2.9 mL of 0.1 M ammonium hydroxide/glycine buffer, pH 10.5.
4. Mix samples thoroughly and determine fluorescence by a fluorometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Methylumbelliferyl phosphate Related Antibodies
Molecular Weight

256.15

Formula

C10H9O6P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1C=C(C)C2=CC=C(OP(O)(O)=O)C=C2O1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : 20.83 mg/mL (81.32 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9040 mL 19.5198 mL 39.0396 mL
5 mM 0.7808 mL 3.9040 mL 7.8079 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 10 mg/mL (39.04 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.9040 mL 19.5198 mL 39.0396 mL 97.5991 mL
5 mM 0.7808 mL 3.9040 mL 7.8079 mL 19.5198 mL
10 mM 0.3904 mL 1.9520 mL 3.9040 mL 9.7599 mL
15 mM 0.2603 mL 1.3013 mL 2.6026 mL 6.5066 mL
20 mM 0.1952 mL 0.9760 mL 1.9520 mL 4.8800 mL
25 mM 0.1562 mL 0.7808 mL 1.5616 mL 3.9040 mL
30 mM 0.1301 mL 0.6507 mL 1.3013 mL 3.2533 mL
40 mM 0.0976 mL 0.4880 mL 0.9760 mL 2.4400 mL
50 mM 0.0781 mL 0.3904 mL 0.7808 mL 1.9520 mL
60 mM 0.0651 mL 0.3253 mL 0.6507 mL 1.6267 mL
80 mM 0.0488 mL 0.2440 mL 0.4880 mL 1.2200 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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4-Methylumbelliferyl phosphate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Methylumbelliferyl3368-04-54-MUP MUPPhosphataseAlkalinephosphatasesfluorogenicsubstratekineticInhibitorinhibitorinhibit

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