2. Immunology/Inflammation
  3. COX
  4. Amfenac Sodium Hydrate

Amfenac Sodium Hydrate is a COX-2 inhibitor.

For research use only. We do not sell to patients.

Amfenac Sodium Hydrate Chemical Structure

Amfenac Sodium Hydrate Chemical Structure

CAS No. : 61618-27-7

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
5 mg USD 32 In-stock
10 mg USD 50 In-stock
50 mg USD 70 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Amfenac Sodium Hydrate Related Antibodies

Top Publications Citing Use of Products

View All COX Isoform Specific Products:

View All Isoforms
COX COX-1 COX-2 COX-3

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Amfenac Sodium Hydrate is a COX-2 inhibitor.

IC50 & Target[1]

COX-2

 

In Vitro

Cells transfected to express COX-2 have a higher proliferation rate than those do not. The addition of Amfenac Sodium Hydrate significantly decreases the proliferation rate of all cell lines. Nitric oxide production by macrophages is inhibited by the addition of melanoma conditioned medium, the addition of Amfenac Sodium Hydrate partially overcomes this inhibition[1]. Results show that Amfenac Sodium Hydrate inhibits the release of B-glucuronidase: 5×10-4 M Amfenac Sodium Hydrate inhibits the release of the enzyme 35.3 and 16.3% in the presence of 10-8, and 10-7 M FMLP, respectively. Addition of 10-4 M Amfenac Sodium Hydrate causes 28.3% inhibition of aggregation of polgmorphonuclear leukocgtes (PMNs) during incubation for 16 min with 10-8 M FMLP[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Amfenac Sodium Hydrate Related Antibodies
Molecular Weight

295.27

Formula

C15H14NNaO4
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(O[Na])CC1=CC=CC(C(C2=CC=CC=C2)=O)=C1N.[H]O[H]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 150 mg/mL (508.01 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (169.34 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3867 mL 16.9337 mL 33.8673 mL
5 mM 0.6773 mL 3.3867 mL 6.7735 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References
Cell Assay
[1]

Human uveal melanoma cell lines are transfected to constitutively express COX-2 and the proliferative rate of these cells using two different methods, with and without the addition of Amfenac Sodium Hydrate, is measured. Nitric oxide production by macrophages is measured after exposure to melanoma-conditioned medium from both groups of cells as well as with and without Amfenac Sodium Hydrate[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.3867 mL 16.9337 mL 33.8673 mL 84.6683 mL
5 mM 0.6773 mL 3.3867 mL 6.7735 mL 16.9337 mL
10 mM 0.3387 mL 1.6934 mL 3.3867 mL 8.4668 mL
15 mM 0.2258 mL 1.1289 mL 2.2578 mL 5.6446 mL
20 mM 0.1693 mL 0.8467 mL 1.6934 mL 4.2334 mL
25 mM 0.1355 mL 0.6773 mL 1.3547 mL 3.3867 mL
30 mM 0.1129 mL 0.5645 mL 1.1289 mL 2.8223 mL
40 mM 0.0847 mL 0.4233 mL 0.8467 mL 2.1167 mL
50 mM 0.0677 mL 0.3387 mL 0.6773 mL 1.6934 mL
60 mM 0.0564 mL 0.2822 mL 0.5645 mL 1.4111 mL
80 mM 0.0423 mL 0.2117 mL 0.4233 mL 1.0584 mL
100 mM 0.0339 mL 0.1693 mL 0.3387 mL 0.8467 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Amfenac Sodium Hydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Amfenac Sodium61618-27-7COXCyclooxygenaseInhibitorinhibitorinhibit

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