1. Metabolic Enzyme/Protease
  2. Tyrosinase Endogenous Metabolite
  3. Arbutin

Arbutin  (Synonyms: β-Arbutin)

Cat. No.: HY-N0192 Purity: ≥98.0%
COA Handling Instructions

Arbutin (β-Arbutin) is a competitive inhibitor of tyrosinase, with Kiapp values of 1.42 mM for monophenolase; 0.9 mM for diphenolase. Arbutin is also used as depigmenting agents. Arbutin is a natural polyphenol isolated from the bearberry plant Arctostaphylos uvaursi, possesses with anti-oxidant, anti-inflammatory and anti-tumor properties.

For research use only. We do not sell to patients.

Arbutin Chemical Structure

Arbutin Chemical Structure

CAS No. : 497-76-7

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Solid + Solvent
10 mM * 1 mL in DMSO
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Solid
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5 g USD 66 In-stock
10 g USD 119 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Arbutin:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Arbutin

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Arbutin (β-Arbutin) is a competitive inhibitor of tyrosinase, with Kiapp values of 1.42 mM for monophenolase; 0.9 mM for diphenolase. Arbutin is also used as depigmenting agents[1]. Arbutin is a natural polyphenol isolated from the bearberry plant Arctostaphylos uvaursi, possesses with anti-oxidant, anti-inflammatory and anti-tumor properties[2][3].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Arbutin (0.3-5.4 mM; 24 hours, 48 hours, 72 hours; B16 murine melanoma cells) inhibites the viability of B16 murine melanoma cells in a time-and dose-dependent manner[2].
? Arbutin (1.4-5.4 mM; 24 hours) increases the apoptosis rate of B16 murine melanoma cell of treatment at a dose of 5.4 mM[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: B16 murine melanoma cells
Concentration: 0.3 mM, 0.7 mM, 1.4 mM, 2.1 mM, 2.9 mM, 3.6 mM, 5.4 mM
Incubation Time: 24 hours, 48 hours or 72 hours
Result: Inhibited the viability of B16 murine melanoma cells in a time- and dose-dependent manner.

Apoptosis Analysis[2]

Cell Line: B16 murine melanoma cells
Concentration: 1.4 mM, 2.9 mM, 5.4 mM
Incubation Time: 24 hours
Result: Caused apoptosis in 19.3% of the cells.
In Vivo

Arbutin (50 mg/kg, 100 mg/kg; oral administration; every day; for 17 days; male C57BL/6 mice) pretreatment exhibits markedly protective effects on ISO-induced cardiac hypertrophy in mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6 mice ( 20-25 g)[3]
Dosage: 50 mg/kg, 100 mg/kg
Administration: Oral administration; every day; for 17 days
Result: Ameliorated the ISO-induced myocardial injury.
Molecular Weight

272.25

Formula

C12H16O7

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]([C@H]([C@@H]([C@@H](CO)O1)O)O)[C@@H]1OC2=CC=C(O)C=C2

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 50 mg/mL (183.65 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 33.33 mg/mL (122.42 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6731 mL 18.3655 mL 36.7309 mL
5 mM 0.7346 mL 3.6731 mL 7.3462 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (367.31 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.6731 mL 18.3655 mL 36.7309 mL 91.8274 mL
5 mM 0.7346 mL 3.6731 mL 7.3462 mL 18.3655 mL
10 mM 0.3673 mL 1.8365 mL 3.6731 mL 9.1827 mL
15 mM 0.2449 mL 1.2244 mL 2.4487 mL 6.1218 mL
20 mM 0.1837 mL 0.9183 mL 1.8365 mL 4.5914 mL
25 mM 0.1469 mL 0.7346 mL 1.4692 mL 3.6731 mL
30 mM 0.1224 mL 0.6122 mL 1.2244 mL 3.0609 mL
40 mM 0.0918 mL 0.4591 mL 0.9183 mL 2.2957 mL
50 mM 0.0735 mL 0.3673 mL 0.7346 mL 1.8365 mL
60 mM 0.0612 mL 0.3061 mL 0.6122 mL 1.5305 mL
80 mM 0.0459 mL 0.2296 mL 0.4591 mL 1.1478 mL
100 mM 0.0367 mL 0.1837 mL 0.3673 mL 0.9183 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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