2. NF-κB Anti-infection Metabolic Enzyme/Protease Immunology/Inflammation
  3. NF-κB HIV Reactive Oxygen Species
  4. Aurothioglucose

Aurothioglucose  (Synonyms: Gold thioglucose)

Cat. No.: HY-A0068 Purity: ≥96.0%
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Aurothioglucose (Gold thioglucose), containing monovalent gold ion, is a potent active-site inhibitor of TrxR1 (thioredoxin reductase 1), with an IC50 of 65 nM. Aurothioglucose inhibits the DNA binding of NF-κB in vitro. Aurothioglucose shows anti-HIV and anti-rheumatic activities.

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Aurothioglucose Chemical Structure

Aurothioglucose Chemical Structure

CAS No. : 12192-57-3

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Solid + Solvent
10 mM * 1 mL in DMSO
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 USD 385 In-stock
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10 mM * 1 mL in DMSO USD 385 In-stock
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Based on 1 publication(s) in Google Scholar

Aurothioglucose Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Aurothioglucose

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Description

Aurothioglucose (Gold thioglucose), containing monovalent gold ion, is a potent active-site inhibitor of TrxR1 (thioredoxin reductase 1), with an IC50 of 65 nM. Aurothioglucose inhibits the DNA binding of NF-κB in vitro. Aurothioglucose shows anti-HIV and anti-rheumatic activities[1][2].

IC50 & Target

IC50: 65 nM (TrxR1)[1]
In Vitro

Aurothioglucose (0-100 μM, 24-72 h) inhibits TrxR1 activity in HeLa cell cytosol but has no effect on the viability of the cells[1].
Aurothioglucose (0-30 μM, 6 h) exhibits very low cytotoxicity on cells[1].
Aurothioglucose (0-20 μM, 24 h) combined with Ebselen (HY-13750) shows a strong synergistic effect, leading to Trx1 (thioredoxin 1) oxidation, reactive oxygen species (ROS) accumulation, and cell death[1].
Aurothioglucose (0-100 μM, 3-12 days) inhibits p24 levels in OM10.1 and Ach2 cells, and inhibits HIV-1 replication in vitro[2].
Aurothioglucose (0-25 μM, 12 days) increases the accumulation of metal gold in a dose-dependent manner[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Aurothioglucose Related Antibodies

Cell Viability Assay[1]

Cell Line: HeLa cells
Concentration: 0, 5, 10, 50, 100 μM
Incubation Time: 24, 48, 72 h
Result: Inhibited TrxR activity by more than 90% at 100 μM in HeLa cells. Cell viability was unaffected by ATG treatment, even at 100 μM after 72 h.

Western Blot Analysis[1]

Cell Line: HeLa cells
Concentration: 0, 5, 10, and 100 μM
Incubation Time: 24 h
Result: Showed no significant oxidation of Trx1 or Trx2 in HeLa cells.

Western Blot Analysis[2]

Cell Line: OM10.1, Ach2 cells
Concentration: 0, 4, 10, 25 and 100 μM
Incubation Time: 3, 6 or 12 days
Result: Significantly inhibited p24 levels. After 12 days of incubation, the viability of cells treated with 10, 25 and 100 μM Aurothioglucose had decreased to 60% of the control.
In Vivo

Aurothioglucose (25 mg/kg, i.p., single) significantly attenuates lung injury and enhances survival in a clinically relevant murine model of ARDS. The protective effects of Aurothioglucose are GSH dependent[3].
Aurothioglucose (300 mg/kg, i.p., single) induces hypothalamic obesity in mice[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult male C3H/HeN mice (8-12 week, LPS/hyperoxia-exposed mice, inflammation/hyperoxia ARDS model)[3]
Dosage: 25 mg/kg
Administration: IP, single, at 12 h after intratracheal LPS administration
Result: Significantly attenuated lung injury, increased lung GCLM expression and GSH levels, and decreased mortality.
Molecular Weight

392.18

Formula

C6H11AuO5S
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O[C@@H]1[C@](O[Au+][SH-]2)([H])C2O[C@H](CO)[C@H]1O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 125 mg/mL (318.73 mM; Need ultrasonic)

DMSO : 6 mg/mL (15.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5498 mL 12.7492 mL 25.4985 mL
5 mM 0.5100 mL 2.5498 mL 5.0997 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥96.0%

COA (249 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.5498 mL 12.7492 mL 25.4985 mL 63.7462 mL
5 mM 0.5100 mL 2.5498 mL 5.0997 mL 12.7492 mL
10 mM 0.2550 mL 1.2749 mL 2.5498 mL 6.3746 mL
15 mM 0.1700 mL 0.8499 mL 1.6999 mL 4.2497 mL
H2O 20 mM 0.1275 mL 0.6375 mL 1.2749 mL 3.1873 mL
25 mM 0.1020 mL 0.5100 mL 1.0199 mL 2.5498 mL
30 mM 0.0850 mL 0.4250 mL 0.8499 mL 2.1249 mL
40 mM 0.0637 mL 0.3187 mL 0.6375 mL 1.5937 mL
50 mM 0.0510 mL 0.2550 mL 0.5100 mL 1.2749 mL
60 mM 0.0425 mL 0.2125 mL 0.4250 mL 1.0624 mL
80 mM 0.0319 mL 0.1594 mL 0.3187 mL 0.7968 mL
100 mM 0.0255 mL 0.1275 mL 0.2550 mL 0.6375 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Aurothioglucose Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Aurothioglucose12192-57-3Gold thioglucoseNF-κBHIVReactive Oxygen SpeciesNuclear factor-κBNuclear factor-kappaBHuman immunodeficiency virusAnti-rheumaticOM10.1Ach2 cellsHIV-1AIDSantiviralrheumatoid arthritisviral latencyHeLa cellsSH-SY5Y cellsHCT116 cellsU2020 cellsDu145 cellsfibroblast cellshypothalamic obesityInhibitorinhibitorinhibit

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