BAY-Y 3118

Cat. No.: HY-U00092 Purity: 99.76%: FAQs
Data Sheet SDS COA Handling Instructions

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BAY-Y 3118 is a new chlorofluoroquinolone with antimicrobial activity.

For research use only. We do not sell to patients.

BAY-Y 3118 Chemical Structure

CAS No. : 151213-16-0

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 209	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 209	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 190	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 320	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 640	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1030	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1700	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Other Forms of BAY-Y 3118:

(R,R)-BAY-Y 3118 Get quote

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Biological Activity
Protocol
Purity & Documentation
References
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Description

BAY-Y 3118 is a new chlorofluoroquinolone with antimicrobial activity.

In Vitro

BAY-Y 3118 is potent against Haemophilhs influenzae, Moraxela catarrhalis, Acinetobacter baumannii, Xanthomonas maltophiia, gram-positive cocci, and anaerobes; MICs for 50%o of the strains (MIC₅₀s) and MIC₉₀s are ≤0.015 and ≤0.015, ≤0.015 and ≤0.015, 0.03 and 2, 0.25 and 0.5, 0.06 and 1, and 0.12 and 0.25 μg/mL, respectively^[1]. The cellular concentration-to-extracellular concentration ratio of BAY-Y 3118 is higher than 6.3 at extracellular concentrations ranging from 2 to 100 mg/L. The uptake of BAY-Y 3118 is rapid, reversible and nonsaturable. The intracellular penetration of BAY-Y 3118 is significantly affected by environmental temperature and cell viability. BAY-Y 3118 reaches high intracellular concentrations within human polymorphonuclear leukocytes (PMNs) and remains active intracellularly^[2]. All strains of L. monocytogenes and other Listeria spp. are highly susceptible; the MICs for these organisms ranges from 0.062 to 0.25 μg/mL. BAY-Y 3118 is rapidly bactericidal in vitro, with a postantibiotic effect occurring for 3 h after removal of the antibiotic. L. monocytogenes is eliminated from infected L929 cells treated with BAY-Y 3118, suggesting a bactericidal effect on the listeriae in these cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Immunocompetent mice are rapidly cured by treatment with 4 mg every 12 h. Concomitantly, the levels of interleukin 6 and gamma interferon in mouse sera decline rapidly. In immunocompetent mice, treatment with 2 mg of BAY-Y 3118 every 12 h results in a greater initial reduction in the listerial counts in the organs than treatment with 2 mg of ampicillin every 12 h. BAY-Y 3118 completely eliminates L. monocytogenes from the livers and spleens of chronically infected nude mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

405.85

Formula

C₂₀H₂₁ClFN₃O₃

CAS No.

151213-16-0

Appearance

Solid

Color

White to light yellow

SMILES

O=C(C1=CN(C2CC2)C3=C(C=C(F)C(N(C4)C[C@]5([H])[C@@]4([H])CCCN5)=C3Cl)C1=O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (82.12 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4640 mL	12.3198 mL	24.6396 mL
5 mM	0.4928 mL	2.4640 mL	4.9279 mL
10 mM	0.2464 mL	1.2320 mL	2.4640 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.76%

Select Batch:

Data Sheet (277 KB) SDS (251 KB)

COA (251 KB) HNMR (286 KB) LCMS (267 KB)

Handling Instructions (2659 KB)

References

[1]. Fass RJ, et al. In vitro activity of Bay y 3118, a new quinolone. Antimicrob Agents Chemother. 1993 Nov;37(11):2348-57. [Content Brief]

[2]. García I, et al. Intracellular penetration and activity of BAY Y 3118 in human polymorphonuclear leukocytes. Antimicrob Agents Chemother. 1994 Oct;38(10):2426-9. [Content Brief]

[3]. Nichterlein T, et al. Bay Y 3118, a new quinolone derivative, rapidly eradicates Listeria monocytogenes from infected mice and L929 cells. Antimicrob Agents Chemother. 1994 Jul;38(7):1501-6. [Content Brief]

Cell Assay ^[3]	Agar plates are prepared and used within 1 day after preparation. The inhibition of bacterial growth is also assessed in tissue culture medium (RPMI 1640 with L-glutamine and 10% fetal calf serum. In brief, 10000 cells of L. monocytogenes EGD are incubated for 8 h in the presence of the antibiotic. Thereafter, the number of bacteria is determined by plating in tryptose agar. The lowest concentration of the antibiotic that inhibited growth in this system is considered the minimal effective concentration in tissue culture medium^[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[3]	Mice: Mice are treated intraperitoneally every 12 h with BAY-Y 3118 (1, 2, or 4 mg per animal in 0.2 mL of PBS starting 6 h postinfection). Control animals receive 0.2 mL of PBS only. At days 1, 3, and 6 postinfection, five mice from each group are killed by cervical dislocation and their spleens and livers are removed aseptically. The organs are homogenized in isotonic saline with Tenbroeck tissue grinders and are further diluted in isotonic saline. The bacterial counts per organ are determined by plating of appropriate dilutions of the homogenates in tryptose agar by a pour plate technique^[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Fass RJ, et al. In vitro activity of Bay y 3118, a new quinolone. Antimicrob Agents Chemother. 1993 Nov;37(11):2348-57. [Content Brief] [2]. García I, et al. Intracellular penetration and activity of BAY Y 3118 in human polymorphonuclear leukocytes. Antimicrob Agents Chemother. 1994 Oct;38(10):2426-9. [Content Brief] [3]. Nichterlein T, et al. Bay Y 3118, a new quinolone derivative, rapidly eradicates Listeria monocytogenes from infected mice and L929 cells. Antimicrob Agents Chemother. 1994 Jul;38(7):1501-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4640 mL	12.3198 mL	24.6396 mL	61.5991 mL
	5 mM	0.4928 mL	2.4640 mL	4.9279 mL	12.3198 mL
	10 mM	0.2464 mL	1.2320 mL	2.4640 mL	6.1599 mL
	15 mM	0.1643 mL	0.8213 mL	1.6426 mL	4.1066 mL
	20 mM	0.1232 mL	0.6160 mL	1.2320 mL	3.0800 mL
	25 mM	0.0986 mL	0.4928 mL	0.9856 mL	2.4640 mL
	30 mM	0.0821 mL	0.4107 mL	0.8213 mL	2.0533 mL
	40 mM	0.0616 mL	0.3080 mL	0.6160 mL	1.5400 mL
	50 mM	0.0493 mL	0.2464 mL	0.4928 mL	1.2320 mL
	60 mM	0.0411 mL	0.2053 mL	0.4107 mL	1.0267 mL
	80 mM	0.0308 mL	0.1540 mL	0.3080 mL	0.7700 mL

BAY-Y 3118 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BAY-Y 3118151213-16-0BacterialInhibitorinhibitorinhibit

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