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  4. Bromobimane

Bromobimane  (Synonyms: Monobromobimane)

Cat. No.: HY-100041 Purity: 99.06%
COA Handling Instructions

Molarity Calculator

Bromobimane (Monobromobimane) is a nonfluorescent and converts into fluorescent products when reacts with thiols. Bromobimane has potential applications in labeling thiols.

For research use only. We do not sell to patients.

Bromobimane Chemical Structure

Bromobimane Chemical Structure

CAS No. : 71418-44-5

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 66 In-stock
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Solid
5 mg USD 38 In-stock
10 mg USD 60 In-stock
25 mg USD 116 In-stock
50 mg USD 190 In-stock
100 mg USD 300 In-stock
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Customer Review

Based on 3 publication(s) in Google Scholar

Bromobimane Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Bromobimane (Monobromobimane) is a nonfluorescent and converts into fluorescent products when reacts with thiols. Bromobimane has potential applications in labeling thiols[1][2].

In Vitro

Bromobimane (mBBr) labeled reactive thiols in cells[1]
(1) Prepare a 100 mM mBBr solution with acetonitrile.
(2) Add 1 mL of 10% cell suspension to 15-25 μL of 100 mM mBBr solution.
(3) After incubation at 37°C for 30-45 min, fluorescence detection was performed.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bromobimane Related Antibodies
Molecular Weight

271.11

Formula

C10H11BrN2O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

Emission (Em)

450
Excitation (Ex)

344
SMILES

O=C1N(C(C(C)=C2C)=O)N2C(CBr)=C1C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 86.6 mg/mL (319.43 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6885 mL 18.4427 mL 36.8854 mL
5 mM 0.7377 mL 3.6885 mL 7.3771 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (7.67 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (7.67 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.06%

COA (255 KB) HNMR (165 KB) LCMS (348 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.6885 mL 18.4427 mL 36.8854 mL 92.2135 mL
5 mM 0.7377 mL 3.6885 mL 7.3771 mL 18.4427 mL
10 mM 0.3689 mL 1.8443 mL 3.6885 mL 9.2213 mL
15 mM 0.2459 mL 1.2295 mL 2.4590 mL 6.1476 mL
20 mM 0.1844 mL 0.9221 mL 1.8443 mL 4.6107 mL
25 mM 0.1475 mL 0.7377 mL 1.4754 mL 3.6885 mL
30 mM 0.1230 mL 0.6148 mL 1.2295 mL 3.0738 mL
40 mM 0.0922 mL 0.4611 mL 0.9221 mL 2.3053 mL
50 mM 0.0738 mL 0.3689 mL 0.7377 mL 1.8443 mL
60 mM 0.0615 mL 0.3074 mL 0.6148 mL 1.5369 mL
80 mM 0.0461 mL 0.2305 mL 0.4611 mL 1.1527 mL
100 mM 0.0369 mL 0.1844 mL 0.3689 mL 0.9221 mL
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Bromobimane Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bromobimane71418-44-5MonobromobimaneFluorescent DyeInhibitorinhibitorinhibit

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