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Bromothymol Blue is a pH indicator.

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Bromothymol Blue Chemical Structure

Bromothymol Blue Chemical Structure

CAS No. : 76-59-5

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Based on 1 publication(s) in Google Scholar

Bromothymol Blue Related Antibodies

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  • Biological Activity

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  • Purity & Documentation

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Description

Bromothymol Blue is a pH indicator.

In Vitro

A first characterization and comparison is done by developing an easy and direct measurement method based on a pH indicator system using Bromothymol Blue (BTB) as the indicator and potassium phosphate as the buffer. A pH-shift assay is developed on the basis of Bromothymol Blue as the pH indicator and potassium phosphate as the buffer component[1]. Three pH indicators are tested for the direct determination of 2- 2-keto-L-gulonic acid (2-KLG) production on a plate. The results show that Bromothymol Blue is superior to the other two indicators in terms of the obvious color change and a suitable pH range (blue to yellow at pH 6.5-7.5). Upon the addition of a Bromothymol Blue solution (0.1%, w/v) to an agar plate, zones surrounding colonies of K. vulgare 07 mutants change their color from blue to yellow because K. vulgare 07 mutants release 2-KLG on agar plates, thereby acidifying surrounding areas around colonies[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bromothymol Blue Related Antibodies
Molecular Weight

624.38

Formula

C27H28Br2O5S
CAS No.
Appearance

Solid

Color

Pale purple to purple

SMILES

O=S1(OC(C2=CC(C(C)C)=C(O)C(Br)=C2C)(C3=CC(C(C)C)=C(O)C(Br)=C3C)C4=CC=CC=C41)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (160.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6016 mL 8.0079 mL 16.0159 mL
5 mM 0.3203 mL 1.6016 mL 3.2032 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References
Kinase Assay
[1]

For all three enzymes, an expression in the 96-deep well scale is performed. Therefore, electrocompetent E. coli ArcticExpress (DE3) cells are transformed with the corresponding plasmid. Single clones are picked using the CP7200 Colony Picker and transferred to 96-deep well plates filled with 1.2 mL autoinduction media by a MicroFlo Select dispenser. After incubation (36 h, 37°C at 1,000 rpm), further processing is done manually. First, 100 μL of cell culture is transferred into a 96-well plate (U-shaped bottom) and harvested by centrifugation (4,570 rpm, 10 min at RT) while the supernatant is discarded. The frozen pellets (1 h at -20°C) are thawed at room temperature for one hour to improve cell lysis. Lysis is continued by the addition of 30 μL lysis buffer (3 h, 1,000 rpm, 37°C) containing 2 mM KPi, pH 7.0, 2 mM MgCl2, 10 μg/mL DNaseI, 100 μg/mL lysozyme. Next, 120 μL buffer (2 mM KPi, pH 7.0) is added followed by centrifugation (3,000 rpm, 15 min at RT). For the photometric measurement, 20 μL of the crude extract is transferred to a 96-well plate (F-shaped bottom) and the reaction is started by adding 180 μL master mix to give a final volume of 200 μL (2.5 mM KPi, pH 7.0, 2 mM MgCl2, 25 μg/mL Bromothymol Blue (BTB) and 5 mM keto-deoxy-D-glucarate). The measurements are carried out for 60 min at 2-min intervals. Depending on the enzyme, different time windows are used for the activity calculation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6016 mL 8.0079 mL 16.0159 mL 40.0397 mL
5 mM 0.3203 mL 1.6016 mL 3.2032 mL 8.0079 mL
10 mM 0.1602 mL 0.8008 mL 1.6016 mL 4.0040 mL
15 mM 0.1068 mL 0.5339 mL 1.0677 mL 2.6693 mL
20 mM 0.0801 mL 0.4004 mL 0.8008 mL 2.0020 mL
25 mM 0.0641 mL 0.3203 mL 0.6406 mL 1.6016 mL
30 mM 0.0534 mL 0.2669 mL 0.5339 mL 1.3347 mL
40 mM 0.0400 mL 0.2002 mL 0.4004 mL 1.0010 mL
50 mM 0.0320 mL 0.1602 mL 0.3203 mL 0.8008 mL
60 mM 0.0267 mL 0.1335 mL 0.2669 mL 0.6673 mL
80 mM 0.0200 mL 0.1001 mL 0.2002 mL 0.5005 mL
100 mM 0.0160 mL 0.0801 mL 0.1602 mL 0.4004 mL
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Bromothymol Blue Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bromothymol Blue76-59-5Fluorescent DyeInhibitorinhibitorinhibit

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