CTX-0294885 hydrochloride

Name: CTX-0294885 hydrochloride
Brand: MedChemExpress
SKU: HY-15985A
Rating: 4.5 (2 reviews)

Cat. No.: HY-15985A Purity: 99.60%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

CTX-0294885 hydrochloride is a broad spectrum kinase inhibitor that can capture 235 kinases from MDA-MB-231 cells, and can capture all members of the AKT family. CTX-0294885 hydrochloride is a powerful reagent for analysis of kinome signaling networks that can be used for the research of diseases like inflammation, diabetes, and cancer.

For research use only. We do not sell to patients.

CTX-0294885 hydrochloride Chemical Structure

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 113	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 113	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 108	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 540	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 780	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of CTX-0294885 hydrochloride:

CTX-0294885 In-stock

2 Publications Citing Use of MCE CTX-0294885 hydrochloride

View All Akt Isoform Specific Products:

View All Isoforms

Akt Akt1 Akt2 Akt3

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

In Vitro

CTX-0294885 hydrochloride is a kinase capture tool in large-scale kinome profiling experiments, with 185 kinases identified as total protein, and 179 identified from TiO₂ enrichment for phosphopeptides in MDA-MB-231 cells (by quantitative MS)^[1].
CTX-0294885 hydrochloridecaptures all members of the AKT family that's not identified from previous studies using other kinase capture reagents^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

474.39

Formula

C₂₂H₂₅Cl₂N₇O

Appearance

Solid

Color

Light yellow to yellow

SMILES

[H]Cl.O=C(NC)C1=CC=CC=C1NC2=NC(NC3=CC=C(N4CCNCC4)C=C3)=NC=C2Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 55 mg/mL (115.94 mM; Need ultrasonic)

DMSO : 9.17 mg/mL (19.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1080 mL	10.5399 mL	21.0797 mL
5 mM	0.4216 mL	2.1080 mL	4.2159 mL
10 mM	0.2108 mL	1.0540 mL	2.1080 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.92 mg/mL (1.94 mM); Clear solution

This protocol yields a clear solution of ≥ 0.92 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (9.2 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.92 mg/mL (1.94 mM); Clear solution

This protocol yields a clear solution of ≥ 0.92 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (9.2 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.92 mg/mL (1.94 mM); Clear solution

This protocol yields a clear solution of ≥ 0.92 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (9.2 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (105.40 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.60%

Select Batch:

Data Sheet (274 KB) SDS (251 KB)

COA (247 KB) LCMS (276 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang L, et al. Characterization of the novel broad-spectrum kinase inhibitor CTx-0294885 as an affinity reagent for mass spectrometry-based kinome profiling. J Proteome Res. 2013 Jul 5;12(7):3104-16. [Content Brief]

Kinase Assay
^[1]

Reactions contains assay buffer, a Nterminally biotinylated peptide substrate, and ATP at a concentration equaling the K_M of the kinase. The compounds (CTX-0294885) are added in a volume of 100 nL from an 11-point-dilution series prepared in DMSO, positive and negative control reactions receiving the same volume of DMSO without compound. The kinases are added at redetermined concentrations, generally ranging between 0.2 nd 8 nM, with the enzyme being omitted from negative ontrol reactions. The reactions are incubated for 90 min at 0 °C and stopped by adding 5 μL of Stop buffer containing streptavidin-coated donor and antiphosphotyrosine acceptor beads. Plates are incubated for 4-6 h before being read on a plate reader^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Zhang L, et al. Characterization of the novel broad-spectrum kinase inhibitor CTx-0294885 as an affinity reagent for mass spectrometry-based kinome profiling. J Proteome Res. 2013 Jul 5;12(7):3104-16. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.1080 mL	10.5399 mL	21.0797 mL	52.6993 mL
	5 mM	0.4216 mL	2.1080 mL	4.2159 mL	10.5399 mL
	10 mM	0.2108 mL	1.0540 mL	2.1080 mL	5.2699 mL
	15 mM	0.1405 mL	0.7027 mL	1.4053 mL	3.5133 mL
H₂O	20 mM	0.1054 mL	0.5270 mL	1.0540 mL	2.6350 mL
	25 mM	0.0843 mL	0.4216 mL	0.8432 mL	2.1080 mL
	30 mM	0.0703 mL	0.3513 mL	0.7027 mL	1.7566 mL
	40 mM	0.0527 mL	0.2635 mL	0.5270 mL	1.3175 mL
	50 mM	0.0422 mL	0.2108 mL	0.4216 mL	1.0540 mL
	60 mM	0.0351 mL	0.1757 mL	0.3513 mL	0.8783 mL
	80 mM	0.0263 mL	0.1317 mL	0.2635 mL	0.6587 mL
	100 mM	0.0211 mL	0.1054 mL	0.2108 mL	0.5270 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.