Carbimazole

Cat. No.: HY-B0558 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Carbimazole is an orally active antithyroid agent which rapidly converts to Methimazole after absorption and prevents thyroid peroxidase enzyme from iodinating and coupling the tyrosine residues on thyroglobulin, hence reducing the production of thyroxine. Carbimazole also displays anti-inflammatory and neuronal-protective activities, suggesting its application for hyperthyroidism and neurological research.

For research use only. We do not sell to patients.

Carbimazole Chemical Structure

CAS No. : 22232-54-8

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Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Carbimazole (0.01-2 mM, 6 h) inhibits the global translation of protein in SK-N-SH cells^[1].
Carbimazole (0.5 mM, 12-72 h) inhibits hypoxic SK-N-SH cell injury and preserve intracellular ATP content in an oxygen-free atmosphere through inhibiting protein synthesis and activating MAPK^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis ^[1]

Cell Line:	SK-N-SH
Concentration:	0.01-2 mM
Incubation Time:	6 h
Result:	Induced the phosphorylation of eEF2 at Thr56 in a dose- and time-dependent manner through the phosphorylation of AMPK, resulting in a global translational inhibition.

In Vivo

Carbimazole (10, 15, 20 mg/animal/day, p.o., 21 d) shows mainly dose-dependent responses of basic physiological parameters in rabbits^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	local breed adult healthy male rabbits
Dosage:	10, 15, 20 mg/animal/day
Administration:	Oral gavage (p.o.) for 21 d
Result:	Decreased rectal temperature, respiration rate, heart rate, the packed cell volume, Hb concentration and total leukocyte count, significantly decreased plasma glucose level; Increased serum levels of total protein, globulins while serum albumin level decreased; Lowered serum urea level while there was an increase in serum urea level with increase in dose level; Increased serum cholesterol level in a dose-dependent manner.

Clinical Trial

Molecular Weight

186.23

Formula

C₇H₁₀N₂O₂S

CAS No.

22232-54-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1C=CN(C)C1=S)OCC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (536.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.3697 mL	26.8485 mL	53.6970 mL
5 mM	1.0739 mL	5.3697 mL	10.7394 mL
10 mM	0.5370 mL	2.6849 mL	5.3697 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (13.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (289 KB) SDS (0 KB)

COA (248 KB) HNMR (240 KB)

Handling Instructions (2659 KB)

References

[1]. Lehane C, et al. Carbimazole is an inhibitor of protein synthesis and protects from neuronal hypoxic damage in vitro. J Pharmacol Exp Ther. 2013 Dec;347(3):781-93. [Content Brief]

[2]. H. Saeed, et al. Effects of Dose Level of Anti-thyroid Drug Carbimazole on Thermoregulation and Blood Constituents in Male Rabbits (Oryctolagus cuniculus). Advances in Research, 2014, 2(3), 129–144.

[3]. Nakashima, T. and A. Taurog, Rapid conversion of carbimazole to methimazole in serum; evidence for an enzymatic mechanism. Clin Endocrinol (Oxf), 1979. 10(6): p. 637-48. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.3697 mL	26.8485 mL	53.6970 mL	134.2426 mL
	5 mM	1.0739 mL	5.3697 mL	10.7394 mL	26.8485 mL
	10 mM	0.5370 mL	2.6849 mL	5.3697 mL	13.4243 mL
	15 mM	0.3580 mL	1.7899 mL	3.5798 mL	8.9495 mL
	20 mM	0.2685 mL	1.3424 mL	2.6849 mL	6.7121 mL
	25 mM	0.2148 mL	1.0739 mL	2.1479 mL	5.3697 mL
	30 mM	0.1790 mL	0.8950 mL	1.7899 mL	4.4748 mL
	40 mM	0.1342 mL	0.6712 mL	1.3424 mL	3.3561 mL
	50 mM	0.1074 mL	0.5370 mL	1.0739 mL	2.6849 mL
	60 mM	0.0895 mL	0.4475 mL	0.8950 mL	2.2374 mL
	80 mM	0.0671 mL	0.3356 mL	0.6712 mL	1.6780 mL
	100 mM	0.0537 mL	0.2685 mL	0.5370 mL	1.3424 mL

Carbimazole Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Carbimazole22232-54-8p38 MAPKantithyroid agentGraves' diseaseMMImethimazoleSK-N-SHhyperthyroidismanti-inflammatoryneuronal-protectiveInhibitorinhibitorinhibit

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