Coelenterazine h  (Synonyms: 2-Deoxycoelenterazine; CLZN-h)

Coelenterazine h (2-Deoxycoelenterazine), a coelenterazine derivative, is a luminescent substrate for RLuc8. Coelenterazine h is more sensitive to Ca²⁺, thus providing a valuable tool for measuring small changes in Ca²⁺ concentrations^[1][2][3][4].

Coelenterazine h (1-10 μM) can be used as the luminescent substrate for RLuc8^[4].
In the measurements of Ca²⁺ binding kinetics of BRAC, emission intensity of Venus (530 nm) from BRAC are monitored at 1 kHz just after rapid mixing of 5 nM BRAC protein with 20 μM coelenterazine-h in various concentration of Ca²⁺ buffer^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

407.46

C₂₆H₂₁N₃O₂

50909-86-9

Solid

Yellow to brown

OC(C=C1)=CC=C1C2=CN(C(C(CC3=CC=CC=C3)=N4)=O)C4=C(N2)CC5=CC=CC=C5

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*The compound is unstable in solutions, freshly prepared is recommended.

• [1]. Jiang T, et al. New bioluminescent coelenterazine derivatives with various C-6 substitutions. Org Biomol Chem. 2017 Aug 23;15(33):7008-7018.  [Content Brief]

  [2]. M R Knight, et al. Imaging calcium dynamics in living plants using semi-synthetic recombinant aequorins. J Cell Biol. 1993 Apr;121(1):83-90.  [Content Brief]

  [3]. Kazushi Suzuki, et al. Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nat Commun. 2016 Dec 14:7:13718.  [Content Brief]

  [4]. Kenta Saito, et al. Auto-luminescent genetically-encoded ratiometric indicator for real-time Ca2+ imaging at the single cell level. PLoS One. 2010 Apr 1;5(4):e9935.  [Content Brief]