2. Neuronal Signaling Anti-infection GPCR/G Protein
  3. Cholinesterase (ChE) Enterovirus Opioid Receptor
  4. Corydaline

Corydaline  (Synonyms: (+)-Corydaline; Corydalin)

Cat. No.: HY-N0923 Purity: 99.70%
COA Handling Instructions

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Corydaline ((+)-Corydaline), an isoquinoline alkaloid isolated from Corydalis yanhusuo, is an AChE inhibitor with an IC50 of 226 µM. Corydaline is a μ-opioid receptor (Ki of 1.23 µM) agonist and inhibits enterovirus 71 (EV71) replication (IC50 of 25.23 µM). Corydaline has anti-angiogenic, anti-allergic and gastric-emptying and antinociceptive activities.

For research use only. We do not sell to patients.

Corydaline Chemical Structure

Corydaline Chemical Structure

CAS No. : 518-69-4

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 77 In-stock
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10 mg USD 110 In-stock
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Based on 1 publication(s) in Google Scholar

Corydaline Related Antibodies

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Description

Corydaline ((+)-Corydaline), an isoquinoline alkaloid isolated from Corydalis yanhusuo, is an AChE inhibitor with an IC50 of 226 µM. Corydaline is a μ-opioid receptor (Ki of 1.23 µM) agonist and inhibits enterovirus 71 (EV71) replication (IC50 of 25.23 µM). Corydaline has anti-angiogenic, anti-allergic and gastric-emptying and antinociceptive activities[1][2][3].

IC50 & Target

AChE

 

μ Opioid Receptor/MOR

 

In Vitro

Corydaline (12.5-50 μM; 24 hours) treatment inhibits EV71 replication by suppressing the COX-2 expression and the phosphorylation of JNK MAPK and P38 MAPK but not ERK MAPK in vitro[2].
? Corydaline could inhibit the viral RNA synthesis in a dose dependent manner[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Corydaline Related Antibodies

Western Blot Analysis[2]

Cell Line: Vero cells infected with EV71
Concentration: 12.5 µM, 25 µM, 50 µM
Incubation Time: 24 hours
Result: Reduced the phosphorylation of P38 MAPK and JNK MAPK and the expression of COX-2.
In Vivo

Corydaline (10 mg/kg; subcutaneous administration; once) treatment shows antinociceptive effects in mice by significantly inhibiting the writhing behavior[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male CD1 mice (30-35 g, 7-8 weeks old) injected with acetic acid[3]
Dosage: 10 mg/kg
Administration: Subcutaneous administration; once
Result: Showed antinociceptive effects in mice.
Molecular Weight

369.45

Formula

C22H27NO4
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

C[C@@H](C1=CC=C2OC)[C@]3([H])C4=CC(OC)=C(OC)C=C4CCN3CC1=C2OC
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (90.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7067 mL 13.5336 mL 27.0673 mL
5 mM 0.5413 mL 2.7067 mL 5.4135 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.77 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.70%

COA (250 KB) HNMR (259 KB) LCMS (88 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7067 mL 13.5336 mL 27.0673 mL 67.6682 mL
5 mM 0.5413 mL 2.7067 mL 5.4135 mL 13.5336 mL
10 mM 0.2707 mL 1.3534 mL 2.7067 mL 6.7668 mL
15 mM 0.1804 mL 0.9022 mL 1.8045 mL 4.5112 mL
20 mM 0.1353 mL 0.6767 mL 1.3534 mL 3.3834 mL
25 mM 0.1083 mL 0.5413 mL 1.0827 mL 2.7067 mL
30 mM 0.0902 mL 0.4511 mL 0.9022 mL 2.2556 mL
40 mM 0.0677 mL 0.3383 mL 0.6767 mL 1.6917 mL
50 mM 0.0541 mL 0.2707 mL 0.5413 mL 1.3534 mL
60 mM 0.0451 mL 0.2256 mL 0.4511 mL 1.1278 mL
80 mM 0.0338 mL 0.1692 mL 0.3383 mL 0.8459 mL
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Corydaline Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Corydaline518-69-4(+)-Corydaline CorydalinCholinesterase (ChE)EnterovirusOpioid ReceptorRhinovirusHRVHRVsHEVHEVsAntiacetylcholinesteraseantiallergicantinociceptiveantibutylcholinesteraseEV71COX-2oral,Inhibitorinhibitorinhibit

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