2. Apoptosis Metabolic Enzyme/Protease
  3. Apoptosis Endogenous Metabolite
  4. Costunolide

Costunolide  (Synonyms: (+)-Costunolide; Costus lactone)

Cat. No.: HY-N0036 Purity: 99.97%
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Costunolide ((+)-Costunolide) is a naturally occurring sesquiterpene lactone, with antioxidative, anti-inflammatory, antiallergic, bone remodeling, neuroprotective, hair growth promoting, anticancer, and antidiabetic properties. Costunolide can induce cell cycle arrest and apoptosis on breast cancer cells.

For research use only. We do not sell to patients.

Costunolide Chemical Structure

Costunolide Chemical Structure

CAS No. : 553-21-9

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10 mM * 1 mL in DMSO
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Based on 5 publication(s) in Google Scholar

Costunolide Related Antibodies

Top Publications Citing Use of Products

    Costunolide purchased from MedChemExpress. Usage Cited in: Gene. 2018 Dec 15;678:261-269.  [Abstract]

    Western blot analysis of mTOR phosphorylation at Ser2448 and Ser2481 with quantification.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Costunolide ((+)-Costunolide) is a naturally occurring sesquiterpene lactone, with antioxidative, anti-inflammatory, antiallergic, bone remodeling, neuroprotective, hair growth promoting, anticancer, and antidiabetic properties. Costunolide can induce cell cycle arrest and apoptosis on breast cancer cells[1][2][3].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Costunolide inhibits the colony formation, migrative and invasive abilities of the H1299 cells in a dose or time dependent manner[2].
    Costunolide (6.7-215.2 μM; 24 hours) inhibits the viability of H1299 cells in a dose-dependent manner, with an IC50 of 23.93 µM[2].
    Costunolide (12.0-48.0 µM; 48 hours) induces apoptosis in H1299 cells[2].
    Costunolide (12-48.0 µM; 6-12 hours) regulates metastasis- and proliferation-associated mRNA expression[2].
    Costunolide regulates epithelial-to-mesenchymal transition (EMT)-associated protein expression[2].
    Costunolide regulates c-Myc mediated apoptosis signaling and 14-3-3-mediated signaling pathways in breast cancer cells[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Costunolide Related Antibodies

    Cell Viability Assay[2]

    Cell Line: H1299 cells
    Concentration: 6.7 μM, 13.5 μM, 26.9 μM, 107.6 μM, 215.2 μM
    Incubation Time: 24 hours
    Result: Inhibited the viability of H1299 cells (MTT assay).

    Apoptosis Analysis[2]

    Cell Line: H1299 cells
    Concentration: 0 µM, 12.0 µM, 24.0 µM, 48.0 µM
    Incubation Time: 48 hours
    Result: Significantly promoted apoptosis at 24.0 µM and 48.0 µM.

    RT-PCR[2]

    Cell Line: H1299 cells
    Concentration: 0 µM, 12.0 µM, 24.0 µM, 48.0 µM
    Incubation Time: 6 hours, 12 hours
    Result: Regulated the metastasis- and proliferation-associated mRNA levels in a dose-dependent manner.

    Western Blot Analysis[2]

    Cell Line: H1299 cells
    Concentration: 0 µM, 12.0 µM, 24.0 µM, 48.0 µM
    Incubation Time: 48 hours
    Result: Significantly inhibited the EMT of H1299 cells.
    In Vivo

    Costunolide (20 mg/kg; i.p; daily; for 30 days) inhibits breast cancer through c-Myc/p53 and AKT/14-3-3 pathway[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 4 weeks old female BALB/c nude mice, MDA-MB-231 cells xenograft mouse models[3]
    Dosage: 20 mg/kg
    Administration: Intraperitoneal injection, daily, for 30 days
    Result: Reduced the expression levels of c-Myc and p-AKT and elevated the expression levels of p53 and p-14-3-3.
    Molecular Weight

    232.32

    Formula

    C15H20O2
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    O=C(O[C@@]1([H])[C@@]2([H])CC/C(C)=C/CC/C(C)=C/1)C2=C
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 49 mg/mL (210.92 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : 25 mg/mL (107.61 mM; ultrasonic and warming and heat to 60°C)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.3044 mL 21.5220 mL 43.0441 mL
    5 mM 0.8609 mL 4.3044 mL 8.6088 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (10.76 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (10.76 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.97%

    COA (260 KB) HNMR (248 KB) LCMS (109 KB) Elemental Analysis Report (118 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 4.3044 mL 21.5220 mL 43.0441 mL 107.6102 mL
    5 mM 0.8609 mL 4.3044 mL 8.6088 mL 21.5220 mL
    10 mM 0.4304 mL 2.1522 mL 4.3044 mL 10.7610 mL
    15 mM 0.2870 mL 1.4348 mL 2.8696 mL 7.1740 mL
    20 mM 0.2152 mL 1.0761 mL 2.1522 mL 5.3805 mL
    25 mM 0.1722 mL 0.8609 mL 1.7218 mL 4.3044 mL
    30 mM 0.1435 mL 0.7174 mL 1.4348 mL 3.5870 mL
    40 mM 0.1076 mL 0.5381 mL 1.0761 mL 2.6903 mL
    50 mM 0.0861 mL 0.4304 mL 0.8609 mL 2.1522 mL
    60 mM 0.0717 mL 0.3587 mL 0.7174 mL 1.7935 mL
    80 mM 0.0538 mL 0.2690 mL 0.5381 mL 1.3451 mL
    100 mM 0.0430 mL 0.2152 mL 0.4304 mL 1.0761 mL
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    Costunolide Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Costunolide553-21-9(+)-Costunolide Costus lactoneApoptosisEndogenous Metaboliteantioxidantsanti-inflammatoryanti-allergicboneregeneratingneuroprotectiveantimicrobialhairgrowthpromotinganticancerantidiabeticInhibitorinhibitorinhibit

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