2. Vitamin D Related/Nuclear Receptor Apoptosis Autophagy
  3. Estrogen Receptor/ERR Apoptosis Autophagy
  4. DPN

DPN  (Synonyms: Diarylpropionitrile)

Cat. No.: HY-12452 Purity: 99.66%
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DPN (Diarylpropionitrile) is a non-steroidal estrogen receptor β (ERβ) selective ligand, with an EC50 of 0.85 nM. DPN has neuroprotective effects in a number of neurological diseases.

For research use only. We do not sell to patients.

DPN Chemical Structure

DPN Chemical Structure

CAS No. : 1428-67-7

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10 mM * 1 mL in DMSO
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Based on 12 publication(s) in Google Scholar

DPN Related Antibodies

Top Publications Citing Use of Products

View All Estrogen Receptor/ERR Isoform Specific Products:

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Estrogen receptor ERα ERβ ERRα ERRβ ERRγ

  • Biological Activity

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Description

DPN (Diarylpropionitrile) is a non-steroidal estrogen receptor β (ERβ) selective ligand, with an EC50 of 0.85 nM. DPN has neuroprotective effects in a number of neurological diseases[1][2].

IC50 & Target[1]

ERβ

0.85 nM (EC50)

In Vitro

DPN has a 70-fold ERα relative binding affinity selectivity, and it is a full ERα agonist with a 78-fold ERα potency selectivity (EC50=0.85 nM for ERβ; EC50=66 nM for ERα)[1].
? DPN (10 nM) prevents morphological alterations from Aβ1-42 (10 μM)-induced toxicity in cultured cortical neurons[2].
? DPN (0.1-100 nM) decreases ROS levels in a non-dose response manner[2].
? DPN (0.1-100 nM) significantly reduces Aβ1-42-stimulated expression of Bax in a non-dose dependent manner[2].
? DPN (0.1-100 nM) reduces activated IL-1 levels induced by Aβ1-42 treatment on cultured cortical neurons[2].
? DPN (0.1-100 nM) suppresses the Aβ1-42-upregulated phosphorylation of JNK and p38[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DPN Related Antibodies
In Vivo

DPN (10 μg; s.c.; daily; for 11 days) increases swimming and decreases immobility in the FST, and increases TPH protein expression in the dorsal raphe nucleus (DR) in rat model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult Sprague-Dawley female rats (220-250 g), ovariectomized animal models[3]
Dosage: 10 μg/rat
Administration: Subcutaneous injections, daily, for 11 days
Result: Increased swimming and decreased immobility in the FST.
Molecular Weight

239.27

Formula

C15H13NO2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC1=CC=C(CC(C#N)C2=CC=C(O)C=C2)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (417.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1794 mL 20.8969 mL 41.7938 mL
5 mM 0.8359 mL 4.1794 mL 8.3588 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (10.45 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (10.45 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.66%

COA (245 KB) LCMS (403 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1794 mL 20.8969 mL 41.7938 mL 104.4845 mL
5 mM 0.8359 mL 4.1794 mL 8.3588 mL 20.8969 mL
10 mM 0.4179 mL 2.0897 mL 4.1794 mL 10.4484 mL
15 mM 0.2786 mL 1.3931 mL 2.7863 mL 6.9656 mL
20 mM 0.2090 mL 1.0448 mL 2.0897 mL 5.2242 mL
25 mM 0.1672 mL 0.8359 mL 1.6718 mL 4.1794 mL
30 mM 0.1393 mL 0.6966 mL 1.3931 mL 3.4828 mL
40 mM 0.1045 mL 0.5224 mL 1.0448 mL 2.6121 mL
50 mM 0.0836 mL 0.4179 mL 0.8359 mL 2.0897 mL
60 mM 0.0697 mL 0.3483 mL 0.6966 mL 1.7414 mL
80 mM 0.0522 mL 0.2612 mL 0.5224 mL 1.3061 mL
100 mM 0.0418 mL 0.2090 mL 0.4179 mL 1.0448 mL
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DPN Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DPN1428-67-7DiarylpropionitrileEstrogen Receptor/ERRApoptosisAutophagyestrogenreceptorbetaAlzheimer'sdiseaseamyloidpeptideantioxidantapoptosisanti-inflammationneuroprotectionanxiety-likebehaviorsInhibitorinhibitorinhibit

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