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Dibenzoyl Thiamine  (Synonyms: Bentiamine)

Cat. No.: HY-B2212 Purity: 99.52%
COA Handling Instructions

Molarity Calculator

Dibenzoyl Thiamine (Bentiamine), a derivative of thiamine, is rapidly absorbed into the body and converted to thiamine.

For research use only. We do not sell to patients.

Dibenzoyl Thiamine Chemical Structure

Dibenzoyl Thiamine Chemical Structure

CAS No. : 299-88-7

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Solid + Solvent
10 mM * 1 mL in DMSO
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Solid
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Customer Review

Based on 1 publication(s) in Google Scholar

Dibenzoyl Thiamine Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Dibenzoyl Thiamine (Bentiamine), a derivative of thiamine, is rapidly absorbed into the body and converted to thiamine.

In Vitro

Dibenzoyl Thiamine is a thiol-type thiamine composed of thiamine and benzoic acid. It is not decomposed by aneurinase (thiamine-decomposing enzyme), making it more suitable for food processing than thiamine hydrochloride[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dibenzoyl Thiamine Related Antibodies
In Vivo

A chronic toxicity study in male Wistar rats for 6 months at dietary levels of up to 1000 ppm, shows Dibenzoyl Thiamine to be without specific toxic effects. A teratogenicity study in rats show Dibenzoyl Thiamine to be without embryotoxic or teratogenic effects. Tests for mutagenicity in bacterial systems, in the dominant lethal assay and in an in vivo cytogenetics test show Dibenzoyl Thiamine to be without mutagenic. Dibenzoyl Thiamine is metabolised to Vitamin Br and benzoic acid, and the benzoic acid moiety is almost quantitatively excreted in the urine, as hippuric acid[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

490.57

Formula

C26H26N4O4S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=CC=C1)S/C(CCOC(C2=CC=CC=C2)=O)=C(N(CC3=CN=C(C)N=C3N)C=O)/C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (101.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0384 mL 10.1922 mL 20.3845 mL
5 mM 0.4077 mL 2.0384 mL 4.0769 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.10 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.10 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.52%

COA (251 KB) HNMR (280 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References
Animal Administration
[1]

Rats: Groups of 65 male and 65 female Sprague-Dawley rats are fed diets containing Dibenzoyl Thiamine at levels of 1000 ppm (Group 1) and 10000 ppm (Group 2). A third group is fed a control diet. The animals are assigned by computer to groups on the basis of body weight, such that mean body weights in all groups are approximately equal. 10 Animals from each group are killed at 52 weeks, the remaining animals at week 105. All signs of ill-health or reaction to treatment are recorded daily. The animals are weighed at weekly intervals[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0384 mL 10.1922 mL 20.3845 mL 50.9611 mL
5 mM 0.4077 mL 2.0384 mL 4.0769 mL 10.1922 mL
10 mM 0.2038 mL 1.0192 mL 2.0384 mL 5.0961 mL
15 mM 0.1359 mL 0.6795 mL 1.3590 mL 3.3974 mL
20 mM 0.1019 mL 0.5096 mL 1.0192 mL 2.5481 mL
25 mM 0.0815 mL 0.4077 mL 0.8154 mL 2.0384 mL
30 mM 0.0679 mL 0.3397 mL 0.6795 mL 1.6987 mL
40 mM 0.0510 mL 0.2548 mL 0.5096 mL 1.2740 mL
50 mM 0.0408 mL 0.2038 mL 0.4077 mL 1.0192 mL
60 mM 0.0340 mL 0.1699 mL 0.3397 mL 0.8494 mL
80 mM 0.0255 mL 0.1274 mL 0.2548 mL 0.6370 mL
100 mM 0.0204 mL 0.1019 mL 0.2038 mL 0.5096 mL
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Dibenzoyl Thiamine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dibenzoyl Thiamine299-88-7BentiamineOthersInhibitorinhibitorinhibit

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