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Diphenylterazine  (Synonyms: DTZ)

Cat. No.: HY-111382 Purity: 98.13%
COA Handling Instructions

Diphenylterazine (DTZ) is a bioluminescence agent. Diphenylterazine alone yielded very little background, leading to excellent signal-to-background ratios.

For research use only. We do not sell to patients.

Diphenylterazine Chemical Structure

Diphenylterazine Chemical Structure

CAS No. : 344940-63-2

Size Price Stock Quantity
1 mg USD 36 In-stock
5 mg USD 110 In-stock
10 mg USD 200 In-stock
25 mg USD 435 In-stock
50 mg USD 700 In-stock
100 mg   Get quote  
200 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 9 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Diphenylterazine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Dec 17;13(1):7799.  [Abstract]

    Diphenylterazine (DTZ) is injected into tumor regions in anesthetized mice and bioluminescence is subsequently imaged.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Diphenylterazine (DTZ) is a bioluminescence agent. Diphenylterazine alone yielded very little background, leading to excellent signal-to-background ratios[1].

    In Vitro

    Diphenylterazine elicits minimal cell toxicity at millimolar concentrations[1].
    Diphenylterazine (DTZ) has high quantum yield, red-shifted emission, favorable in vivo pharmacokinetics and lacks cofactors required for light emission. Yeh AH (2023) used Diphenylterazine (DTZ) as the target substrate of luciferase, and the multinuclear transport factor NTF2-like superfamily as the target topology, to de novo design a small and stable protein scaffold to make the size and shape of the pocket suitable for Diphenylterazine. This method screens out designed luciferases with high selectivity and overcomes the limitations of natural proteins. The catalytic efficiency of the de novo designed luciferase for Diphenylterazine (kcat/Km = 106/M/s) is comparable to that of natural luciferase, but the substrate specificity is higher[2].

    Notes: To make a stock solution for DTZ (Diphenylterazine), first, a premixture is prepared by dissolving 17.6 mg of L-ascorbic acid (HY-B0166) in 10 mL ethanol and 10 mL 1,2-propanediol; next, 1 mg of DTZ is dissolved in 88 μL of the premix, resulting in a 30 mM DTZ stock solution containing 5 mM L-ascorbic acid.
    The stock solution should be stable for a few months in -80°C freezers. It may also be aliquoted to 10-20 µL each at -80°C for the convenience of use. We want to note that this new formulation greatly enhances substrate stability, compared to the conventional acidic alcohol solution[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Diphenylterazine injections into untransfected BALB/c mice do not yield any background emission. The bioluminescence resulting from intraperitoneally injected Diphenylterazine displays extended kinetics[1].
    DTZ (0.3 μmol/mouse (1.13 mg.ml-1/100 ul/mouse); i.v.) treatment can track tumor growth in a xenograft NU/J mouse model[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    377.44

    Formula

    C25H19N3O

    CAS No.
    Appearance

    Solid

    Color

    Yellow to brown

    SMILES

    O=C1C(CC2=CC=CC=C2)=NC3=C(C4=CC=CC=C4)NC(C5=CC=CC=C5)=CN31

    Shipping

    Shipping with dry ice.

    Storage

    -80°C, protect from light, stored under nitrogen

    Solvent & Solubility
    In Vitro: 

    DMF : 11.11 mg/mL (29.44 mM; Need ultrasonic; DMSO can inactivate Diphenylterazine's activity)

    H2O : < 0.1 mg/mL (insoluble; DMSO can inactivate Diphenylterazine's activity)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6494 mL 13.2471 mL 26.4943 mL
    5 mM 0.5299 mL 2.6494 mL 5.2989 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMF    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1.11 mg/mL (2.94 mM); Clear solution

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 2 mg/mL (5.30 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 98.13%

    Dyeing Example
    References
    Cell Assay
    [1]

    BALB/c mice are used and transfected with cells expressing teLuc, Antares, Antares2 and FLuc by injecting cells into the tail vein of BALB/c mice. After the diminishing of the FLuc bioluminescence, 0.3 µmol Diphenylterazine or furimazine is intraperitoneally injected. Mice are imaged with a 1-min exposure per frame over a course of 20 min. The images are processed using the Fiji image analysis software and the frames with highest signals in individual experiments are used for comparison[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMF 1 mM 2.6494 mL 13.2471 mL 26.4943 mL 66.2357 mL
    5 mM 0.5299 mL 2.6494 mL 5.2989 mL 13.2471 mL
    10 mM 0.2649 mL 1.3247 mL 2.6494 mL 6.6236 mL
    15 mM 0.1766 mL 0.8831 mL 1.7663 mL 4.4157 mL
    20 mM 0.1325 mL 0.6624 mL 1.3247 mL 3.3118 mL
    25 mM 0.1060 mL 0.5299 mL 1.0598 mL 2.6494 mL
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    Diphenylterazine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Diphenylterazine
    Cat. No.:
    HY-111382
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