Epimedin B (Synonyms: Epmedin B)

Name: Epimedin B
Brand: MedChemExpress
SKU: HY-N0259
Rating: 4.5 (2 reviews)

Cat. No.: HY-N0259 Purity: 99.81%: FAQs
Data Sheet SDS COA Handling Instructions

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Epimedin B, a component extracted from Epimedii Folium, is reported to have antiosteoporotic activity.

For research use only. We do not sell to patients.

Epimedin B Chemical Structure

CAS No. : 110623-73-9

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 102	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 102	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 32	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 68	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 115	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 416	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 549	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE Epimedin B

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Description

Epimedin B, a component extracted from Epimedii Folium, is reported to have antiosteoporotic activity. IC50 value: Target: In vitro: In vivo: Prednisolone-induced osteoporosis model using zebrafish was used to evaluate the antiosteoporotic activity of micro amount epimedin B. The result showed that 1 μmol·L- 1epimedin B groups were significantly increased when compared with model group; Epimedin B can prevent zebrafish osteoporosis induced by prednisolone [1].

Molecular Weight

808.78

Formula

C₃₈H₄₈O₁₉

CAS No.

110623-73-9

Appearance

Solid

Color

Light yellow to yellow

SMILES

OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](OC2=CC(O)=C(C(C(O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](O)[C@H]3O[C@@]4([H])[C@H](O)[C@@H](O)[C@H](O)CO4)=C(C5=CC=C(OC)C=C5)O6)=O)C6=C2C/C=C(C)/C)O1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (123.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2364 mL	6.1822 mL	12.3643 mL
5 mM	0.2473 mL	1.2364 mL	2.4729 mL
10 mM	0.1236 mL	0.6182 mL	1.2364 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.83 mg/mL (1.03 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.83 mg/mL (1.03 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.81%

Select Batch:

Data Sheet (277 KB) SDS (251 KB)

COA (255 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. ZHAN Yang, et al. Evaluation of Antiosteoporotic Activity for Micro Amount Icariin and Epimedin B Based on the Osteoporosis Model Using Zebrafish. Chinese Pharmaceutical Journal, 2014-01

[2]. Cui L, et al. Metabolite profiles of epimedin B in rats by ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry. J Agric Food Chem. 2013 Apr 17;61(15):3589-99. [Content Brief]

[3]. ZHANG Hua-feng, et al. Effects of microwave radiation on extraction of epimedin B from Epimedii Folium. Chinese Traditional and Herbal Drugs, 2011-09

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2364 mL	6.1822 mL	12.3643 mL	30.9108 mL
	5 mM	0.2473 mL	1.2364 mL	2.4729 mL	6.1822 mL
	10 mM	0.1236 mL	0.6182 mL	1.2364 mL	3.0911 mL
	15 mM	0.0824 mL	0.4121 mL	0.8243 mL	2.0607 mL
	20 mM	0.0618 mL	0.3091 mL	0.6182 mL	1.5455 mL
	25 mM	0.0495 mL	0.2473 mL	0.4946 mL	1.2364 mL
	30 mM	0.0412 mL	0.2061 mL	0.4121 mL	1.0304 mL
	40 mM	0.0309 mL	0.1546 mL	0.3091 mL	0.7728 mL
	50 mM	0.0247 mL	0.1236 mL	0.2473 mL	0.6182 mL
	60 mM	0.0206 mL	0.1030 mL	0.2061 mL	0.5152 mL
	80 mM	0.0155 mL	0.0773 mL	0.1546 mL	0.3864 mL
	100 mM	0.0124 mL	0.0618 mL	0.1236 mL	0.3091 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Epimedin B110623-73-9Epmedin BOthersInhibitorinhibitorinhibit

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Epimedin B (Synonyms: Epmedin B)

Customer Review

Epimedin B Related Antibodies

2 Publications Citing Use of MCE Epimedin B

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

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Dilution Calculator

Complete Stock Solution Preparation Table

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Your Recently Viewed Products:

Customer Review

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