1. Cell Cycle/DNA Damage Cytoskeleton Apoptosis
  2. Microtubule/Tubulin Apoptosis
  3. Estramustine phosphate sodium

Estramustine phosphate sodium, an estradiol analog, is an orally active antimicrotubule chemotherapy agent. Estramustine phosphate sodium depolymerises microtubules by binding to microtubule associated proteins (MAPs) and/or to tubulin. Estramustine phosphate sodium can interfere mitosis, trigger cell death and induce apoptosis, which can be used for the research of cancer like prostate cancer.

For research use only. We do not sell to patients.

Estramustine phosphate sodium Chemical Structure

Estramustine phosphate sodium Chemical Structure

CAS No. : 52205-73-9

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
USD 56 In-stock
Solution
10 mM * 1 mL in Water USD 56 In-stock
Solid
5 mg USD 45 In-stock
10 mg USD 72 In-stock
50 mg USD 119 In-stock
100 mg USD 168 In-stock
200 mg   Get quote  
500 mg   Get quote  

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

Estramustine phosphate sodium, an estradiol analog, is an orally active antimicrotubule chemotherapy agent. Estramustine phosphate sodium depolymerises microtubules by binding to microtubule associated proteins (MAPs) and/or to tubulin. Estramustine phosphate sodium can interfere mitosis, trigger cell death and induce apoptosis, which can be used for the research of cancer like prostate cancer[1][2][3].

In Vitro

Estramustine phosphate sodium (1 µg/ mL, 48 h) suppresses PC3 cell growth[1].
Estramustine phosphate sodium (2 µg/mL, 48 h) elevates phosphatidylserine eversion amount on PC3 cells and induces PC3 cell apoptosis through reducing miR-31[1].
Estramustine phosphate sodium (0-40 µM, 24-72 h) inhibits cell proliferation and tubulin cytoskeleton in RAW 264.7 cells[2].
Estramustine phosphate sodium (10 µM, 24 h) inhibits TGF-β-induced RAW 264.7 cell migration, as well as TGF-β-induced uPA production by inhibiting Smad3 activation[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: PC3 cells
Concentration: 1 µg/ mL
Incubation Time: 48 h
Result: Suppressed PC3 cell growth.

Immunofluorescence[2]

Cell Line: RAW 264.7 cells
Concentration: 10 µM
Incubation Time: 24 h
Result: Disrupted the interphase microtubules.

Cell Migration Assay [2]

Cell Line: 18 h of TGF-β treated RAW 264.7 cells
Concentration: 10 µM
Incubation Time: 24 h
Result: Inhibited TGF-β-Induced Cell Migration.
In Vivo

Estramustine phosphate sodium (Intraperitoneal injection, 4 or 12 mg/kg, a daily dose for 2 weeks) inhibits PAC120 tumor growth 53% by day 35[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Swiss nu/nu (nude) male mice (5-week-old) bearing PAC120 tumors[3]
Dosage: 4 mg/kg, 12 mg/kg
Administration: Intraperitoneal injection; daily; for 2 weeks
Result: Suppressed the development of skin lesions and resulted in a dissociation between DTH response and antibody production.
Animal Model: Human prostate cancer xenograft PAC120[3]
Dosage: 4 or 12 mg/kg, a daily dose for 2 weeks.
Administration: Intraperitoneal injection
Result: Inhibited PAC120 tumor growth 53% by day 35.
Clinical Trial
Molecular Weight

564.35

Formula

C23H30Cl2NNa2O6P

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N(CCCl)CCCl)OC1=CC2=C(C=C1)[C@@]3([H])CC[C@@]4(C)[C@](CC[C@@H]4OP(O[Na])(O[Na])=O)([H])[C@]3([H])CC2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 62.5 mg/mL (110.75 mM; Need ultrasonic)

DMSO : 5 mg/mL (8.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7720 mL 8.8598 mL 17.7195 mL
5 mM 0.3544 mL 1.7720 mL 3.5439 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 65 mg/mL (115.18 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.42%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 1.7720 mL 8.8598 mL 17.7195 mL 44.2988 mL
5 mM 0.3544 mL 1.7720 mL 3.5439 mL 8.8598 mL
H2O 10 mM 0.1772 mL 0.8860 mL 1.7720 mL 4.4299 mL
15 mM 0.1181 mL 0.5907 mL 1.1813 mL 2.9533 mL
20 mM 0.0886 mL 0.4430 mL 0.8860 mL 2.2149 mL
25 mM 0.0709 mL 0.3544 mL 0.7088 mL 1.7720 mL
30 mM 0.0591 mL 0.2953 mL 0.5907 mL 1.4766 mL
40 mM 0.0443 mL 0.2215 mL 0.4430 mL 1.1075 mL
50 mM 0.0354 mL 0.1772 mL 0.3544 mL 0.8860 mL
60 mM 0.0295 mL 0.1477 mL 0.2953 mL 0.7383 mL
80 mM 0.0221 mL 0.1107 mL 0.2215 mL 0.5537 mL
100 mM 0.0177 mL 0.0886 mL 0.1772 mL 0.4430 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Estramustine phosphate sodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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