Fluocinolone (Acetonide)

Name: Fluocinolone (Acetonide)
Brand: MedChemExpress
SKU: HY-B0415
Rating: 4.5 (1 reviews)

Cat. No.: HY-B0415 Purity: 99.59%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Fluocinolone is a glucocorticoid glucocorticoid receptor agonist. Fluocinolone is effective in preventing both lipid accumulation and inflammation. Fluocinolone can promote the proliferation of DPCs and has the potential role in repairing injured pulp tissues. Fluocinolone can be used to study the prevention of chemotherapy-induced peripheral neuropathy caused by Paclitaxel (HY-B0015).

For research use only. We do not sell to patients.

Fluocinolone (Acetonide) Chemical Structure

CAS No. : 67-73-2

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 85	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 85	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 77	In-stock	Estimated Time of Arrival: December 31
1 g	USD 132	In-stock	Estimated Time of Arrival: December 31
5 g	USD 462	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Fluocinolone (0.1-50 μg/mL, 2 days) improves foam cell survival in THP-1 cells^[1].
Fluocinolone (0.1-50 μg/mL, 2 days) inhibits infammatory cytokines secretion and reduces cholesteryl ester accumulation^[1].
Fluocinolone (0.1-100 μmol/L, 24 h) promotes the proliferation of DPCs^[2].
Fluocinolone (1-10 μmol/L, 7 days) promotes the expressions of BSP, OCN, DSPP, and Wnt4, and up-regulates Wnt4 and the dentin-specific marker dentin sialophosphoprotein^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:	DCPs
Concentration:	0.1 μmol/L, 1 μmol/L, 10 μmol/L, 20 μmol/L, 40 μmol/L, 60 μmol/L, 100 μmol/L
Incubation Time:	24 h
Result:	Significantly promoted the growth rate of DPCs of a low concentration.

Western Blot Analysis^[2]

Cell Line:	DCPs
Concentration:	1 μmol/L, 10 μmol/L
Incubation Time:	7 days
Result:	Showed higher expressions of DSPP and Wnt4 protein than negative control.

In Vivo

Fluocinolone (500 μg/kg, Intraperitoneal injection, once a day for 2 weeks) prevents development of significant peripheral neuropathy in a mouse model of PTX-induced^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	PTX-induced peripheral neuropathy model ^[3]
Dosage:	500 μg/kg
Administration:	Intraperitoneal injection (i.p.)
Result:	Prevented a marked reduction in intraepidermal nerve fibers density in the plantar surface of the hind paws.

Molecular Weight

452.49

Formula

C₂₄H₃₀F₂O₆

CAS No.

67-73-2

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@]([C@@]1([H])C[C@@]2([H])[C@@](C[C@H](F)C3=CC4=O)([H])[C@@](F)([C@]3(C=C4)C)[C@@H](O)C5)(OC(C)(C)O1)[C@]25C)CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 31 mg/mL (68.51 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2100 mL	11.0500 mL	22.0999 mL
5 mM	0.4420 mL	2.2100 mL	4.4200 mL
10 mM	0.2210 mL	1.1050 mL	2.2100 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.59%

Select Batch:

Data Sheet (284 KB) SDS (0 KB)

COA (258 KB) LCMS (266 KB)

Handling Instructions (2659 KB)

References

[1]. Nguyen L T H, et al. The potential of fluocinolone acetonide to mitigate inflammation and lipid accumulation in 2D and 3D foam cell cultures [J]. BioMed Research International, 2018, 2018.

[2]. Liu Z, et al. Fluocinolone acetonide promotes the proliferation and mineralization of dental pulp cells [J]. Journal of endodontics, 2013, 39(2): 217-222.

[3]. Cetinkaya-Fisgin A, et al. Identification of fluocinolone acetonide to prevent paclitaxel‐induced peripheral neuropathy [J]. Journal of the Peripheral Nervous System, 2016, 21(3): 128-133.

[4]. Nehmé A, et al. Glucocorticoids with different chemical structures but similar glucocorticoid receptor potency regulate subsets of common and unique genes in human trabecular meshwork cells [J]. BMC medical genomics, 2009, 2(1): 1-14.

[5]. http://en.wikipedia.org/wiki/Fluocinolone_acetonide

[6]. Nehme, A., et al., Glucocorticoids with different chemical structures but similar glucocorticoid receptor potency regulate subsets of common and unique genes in human trabecular meshwork cells. BMC Med Genomics, 2009. 2: p. 58. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2100 mL	11.0500 mL	22.0999 mL	55.2498 mL
	5 mM	0.4420 mL	2.2100 mL	4.4200 mL	11.0500 mL
	10 mM	0.2210 mL	1.1050 mL	2.2100 mL	5.5250 mL
	15 mM	0.1473 mL	0.7367 mL	1.4733 mL	3.6833 mL
	20 mM	0.1105 mL	0.5525 mL	1.1050 mL	2.7625 mL
	25 mM	0.0884 mL	0.4420 mL	0.8840 mL	2.2100 mL
	30 mM	0.0737 mL	0.3683 mL	0.7367 mL	1.8417 mL
	40 mM	0.0552 mL	0.2762 mL	0.5525 mL	1.3812 mL
	50 mM	0.0442 mL	0.2210 mL	0.4420 mL	1.1050 mL
	60 mM	0.0368 mL	0.1842 mL	0.3683 mL	0.9208 mL