2. Apoptosis Autophagy
  3. Apoptosis Autophagy
  4. Gracillin

Gracillin is a steroidal saponin that can be extracted from the roots of the plant and has anti-tumor properties. Gracillin can induce cancer cell apoptosis and autophagy.

For research use only. We do not sell to patients.

Gracillin Chemical Structure

Gracillin Chemical Structure

CAS No. : 19083-00-2

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 92 In-stock
Solution
10 mM * 1 mL in DMSO USD 92 In-stock
Solid
5 mg USD 60 In-stock
10 mg USD 95 In-stock
25 mg USD 175 In-stock
50 mg USD 280 In-stock
100 mg USD 420 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Gracillin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Gracillin

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Gracillin is a steroidal saponin that can be extracted from the roots of the plant and has anti-tumor properties. Gracillin can induce cancer cell apoptosis and autophagy[1][2].

In Vitro

Gracillin (0-10 μM, 6-24 h) shows broad-spectrum inhibitory effects on the cancer cell viability[1].
Gracillin (5 μM) disrupts mitochondrial function, specifically, inhibits ATP production, activates AMPK, inhibits oxygen consumption rate (OCR) ​in H460 and A549 cells[1].
Gracillin (0-10 μM, 6 h) induces ROS generation (indicated by H2DCF-DA) ​in H460, H1299, H226B and A549 cells[1].
Gracillin (0-4 μM, 24 h) blocks cell cycle in G1 phase, and induces apoptosis and autophagy in A549 cells[2][3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Gracillin Related Antibodies

Apoptosis Analysis[2]

Cell Line: A549 cells
Concentration: 0-4 μM
Incubation Time: 24 h
Result: Induced morphological changes.
Apoptosis rate: 6.56%-67.43%.
In Vivo

Gracillin (oral gavage, 10 mg/kg for xenografts and 20 mg/kg for PDX) inhibits tumor growth of xenograft tumors inoculating lung (H1299), prostate (DU145), and colorectum (HCT116) cancer cells, as well as patient-derived xenograft colon and lung tumors[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

885.04

Formula

C45H72O17
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])C[C@@]1([H])[C@@](CC=C5[C@@]6(CC[C@H](O[C@@](O[C@H](CO)[C@@H](O)[C@@H]7O[C@]([C@@H]([C@@H](O)[C@@H]8O)O)([H])O[C@@H]8CO)([H])[C@@H]7O[C@@](O[C@@H](C)[C@H](O)[C@H]9O)([H])[C@@H]9O)C5)C)([H])[C@]6([H])CC2
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (112.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1299 mL 5.6495 mL 11.2989 mL
5 mM 0.2260 mL 1.1299 mL 2.2598 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (2.35 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.13%

COA (255 KB) HNMR (322 KB) LCMS (90 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1299 mL 5.6495 mL 11.2989 mL 28.2473 mL
5 mM 0.2260 mL 1.1299 mL 2.2598 mL 5.6495 mL
10 mM 0.1130 mL 0.5649 mL 1.1299 mL 2.8247 mL
15 mM 0.0753 mL 0.3766 mL 0.7533 mL 1.8832 mL
20 mM 0.0565 mL 0.2825 mL 0.5649 mL 1.4124 mL
25 mM 0.0452 mL 0.2260 mL 0.4520 mL 1.1299 mL
30 mM 0.0377 mL 0.1883 mL 0.3766 mL 0.9416 mL
40 mM 0.0282 mL 0.1412 mL 0.2825 mL 0.7062 mL
50 mM 0.0226 mL 0.1130 mL 0.2260 mL 0.5649 mL
60 mM 0.0188 mL 0.0942 mL 0.1883 mL 0.4708 mL
80 mM 0.0141 mL 0.0706 mL 0.1412 mL 0.3531 mL
100 mM 0.0113 mL 0.0565 mL 0.1130 mL 0.2825 mL
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Gracillin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Gracillin19083-00-2ApoptosisAutophagyInhibitorinhibitorinhibit

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