Fibronectin Adhesion-promoting Peptide  (Synonyms: Heparin Binding Peptide)

Fibronectin Adhesion-promoting Peptide (Heparin Binding Peptide) is one of the heparin-binding amino acid sequences found in the carboxy-terminal heparin-binding domain of fibronectin. It promotes assembly of mesenchymal stem cell (MSC) spheroids into larger aggregates. Fibronectin Adhesion-promoting Peptide directly promotes the adhesion, spreading, and migration of endothelial cells by reacting with heparin binding domains of cells^[1][2].

GRGDS and Fibronectin Adhesion-promoting Peptide (Heparin Binding Peptide: WQPPRARI) micropatterns increase the endothelialisation of prosthetic materials in vitro. Fibronectin Adhesion-promoting Peptide also enhances haptotactic cell migration. The anti-apoptotic effects of fibronectin require the presence of the WQPPRARI sequence, which may be due to synergistic survival signals mediated by this sequence and by the RGD motif^[1].
RDGS and Fibronectin Adhesion-promoting Peptide promote cell adhesion through different activation pathways that induce different forms of adhesion which can be highlighted through the analysis of focal and fibrillar contacts^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1023.19

C₄₇H₇₄N₁₆O₁₀

125720-21-0

Solid

White to off-white

Trp-Gln-Pro-Pro-Arg-Ala-Arg-Ile

WQPPRARI

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Hoesli CA, et al. A fluorophore-tagged RGD peptide to control endothelial cell adhesion to micropatterned surfaces. Biomaterials. 2014 Jan;35(3):879-90.  [Content Brief]

  [2]. Chollet C, et al. Impact of peptide micropatterning on endothelial cell actin remodeling for cell alignment under shear stress. Macromol Biosci. 2012 Dec;12(12):1648-59.  [Content Brief]

  [3]. Lei J, Murphy WL, et al. Combination of Heparin Binding Peptide and Heparin Cell Surface Coatings for Mesenchymal Stem Cell Spheroid Assembly. Bioconjug Chem. 2018 Apr 18;29(4):878-884.  [Content Brief]