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  3. Autophagy
  4. Hoechst 33342 trihydrochloride

Hoechst 33342 trihydrochloride  (Synonyms: bisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride)

Cat. No.: HY-15559A Purity: 99.81%
COA Handling Instructions

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Hoechst 33342 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.

For research use only. We do not sell to patients.

Hoechst 33342 trihydrochloride Chemical Structure

Hoechst 33342 trihydrochloride Chemical Structure

CAS No. : 875756-97-1

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Based on 59 publication(s) in Google Scholar

Other Forms of Hoechst 33342 trihydrochloride:

Hoechst 33342 trihydrochloride Related Antibodies

Top Publications Citing Use of Products

49 Publications Citing Use of MCE Hoechst 33342 trihydrochloride

IF

    Hoechst 33342 trihydrochloride purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2022 Mar 17;18:91-103.  [Abstract]

    The fluorescent images of HUVECs stained with NO fluorescent probe (DAF-FM DA) and Hoechst 33342 after being treated with UCATS under dark conditions or 808 nm NIR irradiation.

    Hoechst 33342 trihydrochloride purchased from MedChemExpress. Usage Cited in: Chem Eng J. 15 December 2022, 138004.

    Apoptotic cells in four groups were stained with Hoechst 33342 (for 10 min) reflected by bright blue nuclei, including hP-MSCs cultured on noncoated, L-Gel-coated (10 nM), and D-Gel coated (10 nM) plates under exposure of 500 μM H2O2 for 5 h except for the normal group, and PBS as control.

    Hoechst 33342 trihydrochloride purchased from MedChemExpress. Usage Cited in: J Lipid Res. 2022 May;63(5):100207.  [Abstract]

    complete medium containing 1 μg/mL BODIPY 500/510 for 3 h and then 2 μg/mL Hoechst 33342 for an additional 2 min.

    Hoechst 33342 trihydrochloride purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Mar 4;11(10):4655-4671.  [Abstract]

    Hoechst 33342 (10 µg/mL) is added to the medium of cultured megakaryocytes for 2 h at 37°C. Cells are stained with the directly coupled monoclonal antibodies PE-anti-CD41 and APC-anti-Mpl for 30 min at 4°C. Ploidy is measured in the CD41+Mpl+ cell population.

    Hoechst 33342 trihydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2021 Oct 4;220(10):e202011153.  [Abstract]

    Chromatin is briefly counterstained with Hoechst 33342 (5 µg/mL) before imaging.

    Hoechst 33342 trihydrochloride purchased from MedChemExpress. Usage Cited in: Aging Cell. 2021 Jul;20(7):e13382.  [Abstract]

    BM cells are incubated with 5 μg/mL Hoechst 33342 in Hank balanced salt solution containing 20 mM HEPES, 5 mM glucose, and 10% fetal bovine serum at 37°C for 45 min, followed by an additional 45 min of incubation with 1 μg/ml Pyronin Y.

    Hoechst 33342 trihydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2021 Jan 22;12(1):77.  [Abstract]

    To evaluate the anti-apoptosis property of extracellular vesicles from hP-MSCs, the Hoechst 33342 (for 10 min) is used to distinguish the apoptotic cells in the three different groups.

    Hoechst 33342 trihydrochloride purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2020 Sep 1;113:289-304.  [Abstract]

    Hoechst 33342 staining (for 6 h) is used to distinguish apoptotic changes in H2O2-treated hP-MSCs.

    Hoechst 33342 trihydrochloride purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2018 Sep 12;10(36):30081-30091.  [Abstract]

    Hoechst 33342 staining shows the survival of HUVECs administrated 100 μg/mL exosomes or CS-Exo upon exposure to 400 μM H2O2.

    Hoechst 33342 trihydrochloride purchased from MedChemExpress. Usage Cited in: Chinese Pharmacological Bulletin. 2018 May; 34(5): 620-626.

    PC12 cells are treated with 1.5 mM Methylglyoxal (MG) for 24 h before pretreatment with Butein (2.5, 5 μM) for 1 h. Propidium iodide (PI) and Hoechst 33342 are used to determine cell apoptosis.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Hoechst 33342 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].

    IC50 & Target

    Dye reagent[1]
    DNA Stain[1]
    In Vitro

    General Protocol
    Preparation of Hoechst working solution
    1.1 Preparation of the stock solution
    Dissolve 10 mg of in 5 mL ddH2O
    Note: It is recommended to store the stock solution at 4°C or -20°C away from light and avoid repetitive freeze-thaw cycles.
    1.2 Preparation of Hoechst working solution
    Dilute the stock solution in serum-free cell culture medium or PBS to obtain final concentration 10 μg/mL Hoechst working solution.
    Note: Please adjust the concentration of Hoechst working solution according to the actual situation.
    1.Cell staining
    2.1 Suspension cells（6-well plate）
    a. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
    b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes.
    c. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
    d. Wash twice with PBS, 5 minutes each time.
    e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
    2.2 Adherent cells
    a. Culture adherent cells on sterile coverslips.
    b. Remove the coverslip from the medium and aspirate excess medium.
    c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes.
    d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

    Storage
    4°C, 1 year. Protect from light

    Precautions
    1. Please adjust the concentration of Hoechst working solution according to the actual situation.
    2. This product is for R&D use only, not for drug, household, or other uses.
    3. For your safety and health, please wear a lab coat and disposable gloves to operate.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Hoechst 33342 trihydrochloride Related Antibodies
    Molecular Weight

    561.93

    Formula

    C27H31Cl3N6O
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to green yellow

    Emission (Em)

    451
    Excitation (Ex)

    356
    SMILES

    [H]Cl.[H]Cl.[H]Cl.CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(OCC)C=C6)N5)=C4)N3)=C2)CC1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *The compound is unstable in solutions, freshly prepared is recommended.

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 46 mg/mL (81.86 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : ≥ 5.6 mg/mL (9.97 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7796 mL 8.8979 mL 17.7958 mL
    5 mM 0.3559 mL 1.7796 mL 3.5592 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 3.33 mg/mL (5.93 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.81%

    COA (247 KB) LCMS (266 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    References
    Cell Assay
    [1]

    Labeling Nuclear DNA with Hoechst 33342[1] Step 1, Dilute the Hoechst stock solution 1:100 in H2O for use in labeling. Step 2, Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS+. Step 3, Incubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. Step 4, Aspirate the labeling solution. Rinse the cells three times in PBS+. Step 5, Mount the coverslips. Step 6, Image the cells (λex ~353 nm, λem ~483 nm for Hoechst 33342)[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.7796 mL 8.8979 mL 17.7958 mL 44.4895 mL
    5 mM 0.3559 mL 1.7796 mL 3.5592 mL 8.8979 mL
    DMSO 10 mM 0.1780 mL 0.8898 mL 1.7796 mL 4.4490 mL
    15 mM 0.1186 mL 0.5932 mL 1.1864 mL 2.9660 mL
    20 mM 0.0890 mL 0.4449 mL 0.8898 mL 2.2245 mL
    25 mM 0.0712 mL 0.3559 mL 0.7118 mL 1.7796 mL
    30 mM 0.0593 mL 0.2966 mL 0.5932 mL 1.4830 mL
    40 mM 0.0445 mL 0.2224 mL 0.4449 mL 1.1122 mL
    50 mM 0.0356 mL 0.1780 mL 0.3559 mL 0.8898 mL
    60 mM 0.0297 mL 0.1483 mL 0.2966 mL 0.7415 mL
    80 mM 0.0222 mL 0.1112 mL 0.2224 mL 0.5561 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Hoechst 33342 trihydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Hoechst 33342 trihydrochloride875756-97-1bisBenzimide H 33342 trihydrochloride HOE 33342 trihydrochlorideAutophagyHoechstDNAcellular dyeblue fluorescenceInhibitorinhibitorinhibit

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