2. Apoptosis Autophagy
  3. Apoptosis Autophagy
  4. Hydroxysafflor yellow A

Hydroxysafflor yellow A  (Synonyms: Safflomin A; HSYA)

Cat. No.: HY-N0567 Purity: 98.95%
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Hydroxysafflor yellow A (Safflomin A) is a natural product of flavonoids isolated from safflower. Hydroxysafflor yellow A can inhibit cell proliferation and promote apoptosis through the autophagy pathway. Hydroxysafflor yellow A has anti-inflammatory, antioxidant and antitumor effects. Hydroxysafflor yellow A can be used in the study of cardiovascular disease.

For research use only. We do not sell to patients.

Hydroxysafflor yellow A Chemical Structure

Hydroxysafflor yellow A Chemical Structure

CAS No. : 78281-02-4

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 89 In-stock
Solution
10 mM * 1 mL in DMSO USD 89 In-stock
Solid
5 mg USD 66 In-stock
10 mg USD 106 In-stock
50 mg USD 343 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Based on 10 publication(s) in Google Scholar

Hydroxysafflor yellow A Related Antibodies

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Description

Hydroxysafflor yellow A (Safflomin A) is a natural product of flavonoids isolated from safflower. Hydroxysafflor yellow A can inhibit cell proliferation and promote apoptosis through the autophagy pathway. Hydroxysafflor yellow A has anti-inflammatory, antioxidant and antitumor effects. Hydroxysafflor yellow A can be used in the study of cardiovascular disease[1][2][3][4][5][6].

In Vitro

Hydroxysafflor yellow A (10 μM, 24 h) Inhibition of LPS-induced proliferation and migration of vascular smooth muscle cells by toll-like receptor-4 pathway[1].
Hydroxysafflor yellow A (0.01-10 mg/L, 4-96 h) can promote the expression of fat-soluble specific enzyme HSL by increasing the activity of HSL promoter and inhibit the proliferation and adipogenesis of 3T3-L1 preadipocytes[2].
Hydroxysafflor yellow A (1-100 μM), 12-24 h) decreases LPS-induced EC inflammatory damage by inhibiting p38 MAPK phosphorylation, NF-κB activation, high expression of inflammatory factors and leukocyte adhesion to EC[3].
Hydroxysafflor yellow A (20-160 μM, 24-48 h) inhibits cell proliferation and promotes apoptosis by blocking the autophagy flux in liver cancer cells[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hydroxysafflor yellow A Related Antibodies

Cell Migration Assay [1]

Cell Line: VSMCs
Concentration: 0.1, 1, 10,100 μM
Incubation Time: 24 h
Result: Inhibited LPS-induced VSMCs proliferation and migration with the concentration of 10 μM.

Western Blot Analysis[1]

Cell Line: VSMCs
Concentration: 10 μM
Incubation Time: 24 h
Result: Inhibited LPS-induced upregulation of pro-inflammatory factors (TNF-α, IL-6, and IL-8).
Inhibited LPS-induced upregulation of TLR-4 and activation of Rac1/Akt pathway.

Cell Viability Assay[2]

Cell Line: 3T3-L1
Concentration: 0.01, 0.1, 1, 10 mg/L
Incubation Time: 4, 8, 24, 48, 72, 96 h
Result: Inhibit the cell viability in a dose and time-dependent manner.

Apoptosis Analysis[6]

Cell Line: HepG2, Huh7
Concentration: 20, 40, 80, 160 μM
Incubation Time: 24 h
Result: Suppressed the growth and induced apoptotic cell death in both concentration and time-dependent manners.
Augmented the induction of autophagosomes.
In Vivo

Hydroxysafflor yellow A (2.5 or 10 mg/kg/day, intraperitoneal injection for 6 weeks) protects the liver from long-term alcohol damage by enhancing antioxidant capacity and inhibiting TGF-β1 expression[4].
Hydroxysafflor yellow A (30, 48, 76.8 mg/kg, intraperitoneal injection) has a protective effect on inflammatory damage in rats with chronic obstructive pulmonary disease by reducing NF-κB and p38MAPK signal transduction[5].
Hydroxysafflor yellow A (1.135, 2.25 mg/kg, intraperitoneal injection) can inhibit tumor growth and block autophagy of liver cancer cells in tumor models of nude mice[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Liver injury model induced by alcohol[4]
Dosage: 2.5 or 10 mg/kg
Administration: i.p. daily for 6 weeks
Result: Decreased the level of ALT and AST.
Decreased the levels of HA, LN, and III-C.
Increased the activities of SOD and GPx and decreased the level of TGF-β1 expression.
Animal Model: Chronic obstructive pulmonary disease (COPD)[5]
Dosage: 30, 48, 76.8 mg/kg
Administration: i.p.
Result: Inhibited the elevated IL-6, IL-1β, TNF-α, ICAM-1 and VCAM-1 mRNA Levels.
Inhibited the increased phosphorylation and NF-κB p65 levels.
Molecular Weight

612.53

Formula

C27H32O16
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

OC(C(O)=C1C(/C=C/C2=CC=C(O)C=C2)=O)([C@]([C@@H]([C@@H](O)[C@@H]3O)O)([H])O[C@@H]3CO)C(O)=C([C@@H]([C@@H]([C@@H](O)[C@@H]4O)O)O[C@@H]4CO)C1=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (408.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 33.33 mg/mL (54.41 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6326 mL 8.1629 mL 16.3257 mL
5 mM 0.3265 mL 1.6326 mL 3.2651 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (3.40 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (3.40 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 98.95%

COA (256 KB) HNMR (309 KB) RP-HPLC (209 KB) MS (70 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.6326 mL 8.1629 mL 16.3257 mL 40.8143 mL
5 mM 0.3265 mL 1.6326 mL 3.2651 mL 8.1629 mL
10 mM 0.1633 mL 0.8163 mL 1.6326 mL 4.0814 mL
15 mM 0.1088 mL 0.5442 mL 1.0884 mL 2.7210 mL
20 mM 0.0816 mL 0.4081 mL 0.8163 mL 2.0407 mL
25 mM 0.0653 mL 0.3265 mL 0.6530 mL 1.6326 mL
30 mM 0.0544 mL 0.2721 mL 0.5442 mL 1.3605 mL
40 mM 0.0408 mL 0.2041 mL 0.4081 mL 1.0204 mL
50 mM 0.0327 mL 0.1633 mL 0.3265 mL 0.8163 mL
DMSO 60 mM 0.0272 mL 0.1360 mL 0.2721 mL 0.6802 mL
80 mM 0.0204 mL 0.1020 mL 0.2041 mL 0.5102 mL
100 mM 0.0163 mL 0.0816 mL 0.1633 mL 0.4081 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Hydroxysafflor yellow A Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hydroxysafflor yellow A78281-02-4Safflomin A HSYAApoptosisAutophagyHSYAProliferation3T3-L1VSMCsECHepG2Huh7Chronic obstructive pulmonary diseaseInhibitorinhibitorinhibit

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