1. Metabolic Enzyme/Protease Anti-infection
  2. Phosphatase Orthopoxvirus
  3. Idoxuridine

Idoxuridine  (Synonyms: 5-Iodo-2′-deoxyuridine; 5-IUdR; IdUrd)

Cat. No.: HY-B0307 Purity: 99.70%
COA Handling Instructions

Idoxuridine (5-Iodo-2′-deoxyuridine, 5-IUdR, IdUrd) is an iodinated thymidine analogue that competitively inhibits phosphorylases. Idoxuridine can inhibit viral activity, particularly viral eye infections, including herpes simplex keratitis, by inhibiting DNA polymerase and affecting viral replication. Idoxuridine against feline herpesvirus has the IC50 value of 4.3 μM. Idoxuridine shows anti-orthopoxvirus activity.

For research use only. We do not sell to patients.

Idoxuridine Chemical Structure

Idoxuridine Chemical Structure

CAS No. : 54-42-2

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
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Solid
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1 g USD 55 In-stock
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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Idoxuridine:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Idoxuridine (5-Iodo-2′-deoxyuridine, 5-IUdR, IdUrd) is an iodinated thymidine analogue that competitively inhibits phosphorylases. Idoxuridine can inhibit viral activity, particularly viral eye infections, including herpes simplex keratitis, by inhibiting DNA polymerase and affecting viral replication. Idoxuridine against feline herpesvirus has the IC50 value of 4.3 μM[1]. Idoxuridine shows anti-orthopoxvirus activity.

IC50 & Target[4]

HSV-1

 

In Vitro

Idoxuridine (2-10 μM, 72 hours) has the IC50 value of 4.3 μM of antiviral[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: Crandell-Reese feline kidney (CRFK) cells
Concentration: 2-10 μM
Incubation Time: 72 hours
Result: Showed the IC50 value of 4.3 μM.

Cell Cytotoxicity Assay[1]

Cell Line: Crandell-Reese feline kidney (CRFK) cells
Concentration: 5-50 μM
Incubation Time: 72 hours
Result: Reduced by 10.8% relatively in CRFK cells.
In Vivo

Idoxuridine (intraperitoneal injection, 50-200 mg/kg, 3 times, 3 hours interval) can stimulate the production of hemolysin plaque-forming cells (HPFC) to sheep red blood cells (SRBC) in C3HeB/FeJ female and male mice and A/J male mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C3HeB/FeJ female and male mice and A/J male mice, aged 2 to 4 months[2]
Dosage: 50-200 mg/kg
Administration: Intraperitoneal injection, 3 times, 3 hours interval
Result: Stimulated the production of hemolysin plaque-forming cells (HPFC) to sheep red blood cells (SRBC) in the dose range of 50-200 mg/kg.
Clinical Trial
Molecular Weight

354.10

Formula

C9H11IN2O5

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N1)N([C@H]2C[C@H](O)[C@@H](CO)O2)C=C(I)C1=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (353.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8241 mL 14.1203 mL 28.2406 mL
5 mM 0.5648 mL 2.8241 mL 5.6481 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (5.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (5.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.95%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8241 mL 14.1203 mL 28.2406 mL 70.6015 mL
5 mM 0.5648 mL 2.8241 mL 5.6481 mL 14.1203 mL
10 mM 0.2824 mL 1.4120 mL 2.8241 mL 7.0602 mL
15 mM 0.1883 mL 0.9414 mL 1.8827 mL 4.7068 mL
20 mM 0.1412 mL 0.7060 mL 1.4120 mL 3.5301 mL
25 mM 0.1130 mL 0.5648 mL 1.1296 mL 2.8241 mL
30 mM 0.0941 mL 0.4707 mL 0.9414 mL 2.3534 mL
40 mM 0.0706 mL 0.3530 mL 0.7060 mL 1.7650 mL
50 mM 0.0565 mL 0.2824 mL 0.5648 mL 1.4120 mL
60 mM 0.0471 mL 0.2353 mL 0.4707 mL 1.1767 mL
80 mM 0.0353 mL 0.1765 mL 0.3530 mL 0.8825 mL
100 mM 0.0282 mL 0.1412 mL 0.2824 mL 0.7060 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Idoxuridine
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