1. Membrane Transporter/Ion Channel
    Neuronal Signaling
    PI3K/Akt/mTOR
  2. GABA Receptor
    mTOR
    PI3K
    Akt

Jujuboside A 

Cat. No.: HY-N0659
Handling Instructions

Jujuboside A is a glycoside extracted from Semen Ziziphi Spinosae, a Chinese herbal medicine used to treat insomnia and anxiety.

For research use only. We do not sell to patients.
Jujuboside A Chemical Structure

Jujuboside A Chemical Structure

CAS No. : 55466-04-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO $212 In-stock
5 mg $100 In-stock
10 mg $160 In-stock
25 mg $360 In-stock
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • References

Description

Jujuboside A is a glycoside extracted from Semen Ziziphi Spinosae, a Chinese herbal medicine used to treat insomnia and anxiety.

IC50 & Target

GABA Receptor, mTOR, PI3K, Akt[1][2]

In Vitro

Jujuboside A at the low dose of 41 μM (about 0.05 g/L) induces significant increase of GABA(A) receptor α1, α5, β2 subunit mRNAs in both 24 and 72h treatments. Jujuboside A at the high dose of 82 μM (about 0.1 g/L) significantly increases GABA(A) receptor α1, α5 subunit mRNA levels and decreases β2 subunit mRNA level at 24h treatment, and decreases GABA(A) receptor subunit α1, β2 mRNAs expression at 72h treatment[1]. Jujuboside A pretreatment could reverse the reduction of cell viability and better the injury of H9C2 cells induced by ISO. Jujuboside A could accelerate the phosphorylation of PI3K, Akt, and mTOR. Jujuboside A could significantly decrease the ratio of microtubule-associated protein LC3-II/I in H9C2 cells[2].

In Vivo

During daytime (9:00-15:00), jujubosides significantly increases the total sleep and rapid eye movement (REM) sleep without significant influence on non-REM (NREM) sleep. During nighttime (21:00-3:00), jujubosides significantly increases the total sleep and NREM sleep especially the light sleep while shows no significant effect on REM sleep and slow wave sleep (SWS)[3]. Intracerebroventricular treatment with Jujuboside A significantly mitigates learning and memory impairment in mice induced by Aβ1–42 as measured by the Y-maze, active avoidance and Morris water maze. Intracerebroventricular treatment with Jujuboside A reduces the level of Aβ1–42 in hippocampus, significantly inhibits the activities of acetylcholinesterase (AChE) and NO, and decreases the amount of the increased malondialdehyde (MDA) in the hippocampus and cerebral cortex of mice treated with intracerebroventricular injection of Aβ1–42[4].

References
Preparing Stock Solutions
Concentration Volume (DMSO) Mass 1 mg 5 mg 10 mg
1 mM 0.8283 mL 4.1413 mL 8.2826 mL
5 mM 0.1657 mL 0.8283 mL 1.6565 mL
10 mM 0.0828 mL 0.4141 mL 0.8283 mL
Cell Assay
[1]

Jujuboside A is dissolved in D-PBS solution[1].

After 7 days in culture in vitro, cells are exposed continuously for the next 24 or 72 h to the culture medium with either Jujuboside A or diazepam, or neither of them. Vehicle is added for the control group; 10 μM diazepam is added for the diazepam group; Jujuboside A 82 μM (about 0.1 g/L) and 41 μM (about 0.05 g/L) are added for the JuA-H (high dose Jujuboside A) and the JuA-L (low dose Jujuboside A) groups respectively. Total RNA is isolated from cells for further analysis[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Jujuboside A is dissolved in physiological saline solution at a stock concentration of 0.2 mg/mL and 2.0 mg/mL[1].

Mouse: Cognitive impairment of mice is induced by ICV injection of Aβ1–42. Then mice are given intracerebroventricular (ICV) injection of Jujuboside A (0.02 and 0.2 mg/kg) for five consecutive days. Y-maze, active avoidance and Morris water maze tests are performed on mice[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

1207.35

Formula

C₅₈H₉₄O₂₆

CAS No.

55466-04-1

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Jujuboside A
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HY-N0659
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