LMP744 (Synonyms: MJ-III65; NSC706744)

Cat. No.: HY-U00248 Purity: 97.00%: FAQs
Data Sheet SDS COA Handling Instructions

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LMP744 (MJ-III65) is a DNA intercalator and Topoisomerase I (Top1) inhibitor with antitumor activity.

For research use only. We do not sell to patients.

LMP744 Chemical Structure

CAS No. : 308246-52-8

Size	Price	Stock	Quantity
1 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 290	Get quote	Estimated Time of Arrival: December 31
10 mg	USD 450	Get quote	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Other Forms of LMP744:

LMP744 hydrochloride In-stock

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Topoisomerase Topo I Topo II

Biological Activity
Purity & Documentation
References
Customer Review

Description

LMP744 (MJ-III65) is a DNA intercalator and Topoisomerase I (Top1) inhibitor with antitumor activity^[1].

IC₅₀ & Target^[1]

Top1

In Vitro

The GI₅₀ value of LMP744 (MJ-III-65) for NCI60 cells is 0.1 μM^[2].
LMP744 (0.1-5 μM, 3 days) induces dose-dependent accumulation of cells in the S and G₂ phases of the cell cycle^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	P388 and P388 Top1-deficient murine leukemia cells
Concentration:	0.1-100 μM
Incubation Time:	3 days
Result:	Induced dose-dependent accumulation of cells in the S and G₂ phases of the cell cycle.

In Vivo

LMP744 (MJ-III65) (10-50 mg/kg) administered i.v. push once a week for 4 weeks in nude mice moderately actives against human A253 and FaDu tumor xenografts without significant toxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Athymic nude mice (nu/nu, female, 20-25 g, 8-12 weeks old) transplanted with A253 and FaDu human head and neck xenografts^[1].
Dosage:	10, 25, or 50 mg/kg/week, 4 weeks
Administration:	I.V. push via tail vein
Result:	Moderately actived against human A253 and FaDu tumor xenografts without significant toxicity.

Clinical Trial

Molecular Weight

452.46

Formula

C₂₄H₂₄N₂O₇

CAS No.

308246-52-8

Appearance

Solid

Color

Brown to dark brown

SMILES

OCCNCCCN1C2=C(C(C3=CC(OCO4)=C4C=C23)=O)C5=CC(OC)=C(OC)C=C5C1=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 4.17 mg/mL (9.22 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2101 mL	11.0507 mL	22.1014 mL
5 mM	0.4420 mL	2.2101 mL	4.4203 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 97.00%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (249 KB) HNMR (274 KB) RP-HPLC (231 KB) MS (198 KB)

Handling Instructions (2659 KB)

References

[1]. Antony S, et al. Cellular topoisomerase I inhibition and antiproliferative activity by MJ-III-65 (NSC 706744), an indenoisoquinoline topoisomerase I poison. Mol Pharmacol. 2005 Feb;67(2):523-30. [Content Brief]

[2]. Antony S, et al. Novel indenoisoquinolines NSC 725776 and NSC 724998 produce persistent topoisomerase I cleavage complexes and overcome multidrug resistance. Cancer Res. 2007 Nov 1;67(21):10397-405. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2101 mL	11.0507 mL	22.1014 mL	55.2535 mL
DMSO	5 mM	0.4420 mL	2.2101 mL	4.4203 mL	11.0507 mL

LMP744 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LMP744308246-52-8MJ-III65 NSC706744LMP 744LMP-744NSC706744NSC 706744NSC-706744TopoisomeraseInhibitorinhibitorinhibit

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