2. Metabolic Enzyme/Protease NF-κB
  3. MALT1
  4. MLT-748

MLT-748 is a potent, selective and allosteric inhibitor of MALT1, binds MALT1 in the allosteric Trp580 pocket, with an IC50 of 5 nM.

For research use only. We do not sell to patients.

MLT-748 Chemical Structure

MLT-748 Chemical Structure

CAS No. : 1832578-30-9

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 325 In-stock
Solution
10 mM * 1 mL in DMSO USD 325 In-stock
Solid
5 mg USD 300 In-stock
10 mg USD 480 In-stock
50 mg USD 950 In-stock
100 mg USD 1480 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

MLT-748 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

MLT-748 is a potent, selective and allosteric inhibitor of MALT1, binds MALT1 in the allosteric Trp580 pocket, with an IC50 of 5 nM[1].

IC50 & Target

IC50: 5 nM (MALT1)[1]
Kd: 13 nM (MALT1(329-728)-W580S), 42 nM (MALT1(329-728))[1]
In Vitro

MLT-748 reversibly binds to human mutant MALT1(329-728)-W580S (Kd, 13 nM) with affinity similar to that of the wild type MALT1(329-728) (Kd, 42 nM)[1].
MLT-748 (0-2 μM) stabilizes cellular MALT1-W580S, with an EC50 of 69 nM[1].
MLT-748 (2 μM, 24 hours) increases the phosphorylation of p65 and IκBα in MALT1mut/mut patient immortalized B cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MLT-748 Related Antibodies

Western Blot Analysis

Cell Line: MALT1mut/mut patient immortalized B cells
Concentration: 2 µM
Incubation Time: 24 hours
Result: Increased NF-κB signaling phosphorylation of both p65 and IκBα as early as 5 min after stimulation with PMA and ionomycin.
Molecular Weight

492.32

Formula

C19H19Cl2N9O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC(Cl)=C(N2N=CC=N2)N=C1)NC3=C([C@@H](OC)[C@H](OC)C)N4C(N=C3)=CC(Cl)=N4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (406.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0312 mL 10.1560 mL 20.3120 mL
5 mM 0.4062 mL 2.0312 mL 4.0624 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  5% DMSO    95% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.08 mM); Clear solution

  • Protocol 2

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.22 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0312 mL 10.1560 mL 20.3120 mL 50.7800 mL
5 mM 0.4062 mL 2.0312 mL 4.0624 mL 10.1560 mL
10 mM 0.2031 mL 1.0156 mL 2.0312 mL 5.0780 mL
15 mM 0.1354 mL 0.6771 mL 1.3541 mL 3.3853 mL
20 mM 0.1016 mL 0.5078 mL 1.0156 mL 2.5390 mL
25 mM 0.0812 mL 0.4062 mL 0.8125 mL 2.0312 mL
30 mM 0.0677 mL 0.3385 mL 0.6771 mL 1.6927 mL
40 mM 0.0508 mL 0.2539 mL 0.5078 mL 1.2695 mL
50 mM 0.0406 mL 0.2031 mL 0.4062 mL 1.0156 mL
60 mM 0.0339 mL 0.1693 mL 0.3385 mL 0.8463 mL
80 mM 0.0254 mL 0.1269 mL 0.2539 mL 0.6347 mL
100 mM 0.0203 mL 0.1016 mL 0.2031 mL 0.5078 mL
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MLT-748 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MLT-7481832578-30-9MLT748MLT 748MALT1mucosa associated lymphoid tissue lymphoma translocation gene 1Inhibitorinhibitorinhibit

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