2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species
  4. NucPE1

NucPE1  (Synonyms: Nuclear Peroxy Emerald 1)

Cat. No.: HY-101859 Purity: ≥98.0%
COA Handling Instructions

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NucPE1 (Nuclear Peroxy Emerald 1) is a nuclear-localized fluorescent hydrogen peroxide that is specifically localized to cellular nuclei without appended targeting moieties.

For research use only. We do not sell to patients.

NucPE1 Chemical Structure

NucPE1 Chemical Structure

CAS No. : 1404091-23-1

Size Price Stock Quantity
1 mg USD 180 In-stock
5 mg USD 540 In-stock
10 mg USD 900 In-stock
50 mg USD 2520 In-stock
100 mg USD 3720 In-stock
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Based on 1 publication(s) in Google Scholar

NucPE1 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE NucPE1

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

NucPE1 (Nuclear Peroxy Emerald 1) is a nuclear-localized fluorescent hydrogen peroxide that is specifically localized to cellular nuclei without appended targeting moieties.

In Vitro

NucPE1 features two major visible region absorptions (λabs=468 nm, ε=27,300 M-1cm-1; λabs=490 nm, ε=26,000 M-1cm-1) and a weak emission (λem=530 nm, Φ=0.117). Reaction of NucPE1 with H2O2 triggers a fluorescence increase upon its conversion to fluorophore NucPE1, which possesses one major absorption band at 505 nm (ε=19,100 M-1cm-1) with enhanced emission (λem=530 nm, Φ=0.626). NucPE1 selectively accumulates in the nuclei of a variety of mammalian cell lines as well as in whole model organisms like C. clegans, where it can respond to subcellular changes in H2O2 fluxes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NucPE1 Related Antibodies
In Vivo

NucPE1 maintains the ability to selectively target nuclei in vivo. NucPE1 imaging reveals a reduction in nuclear H2O2 levels in worms overexpressing sir-2.1 compared to wildtype congeners, supporting a link between this longevity-promoting sirtuin protein and enhanced regulation of nuclear ROS pools[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

483.36

Formula

C29H30BNO5
CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

530
Excitation (Ex)

490
SMILES

O=C1OC2(C3=C(OC4=C2C=CC(B5OC(C)(C)C(C)(C)O5)=C4)C=C(NCC)C(C)=C3)C6=C1C=CC=C6
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (51.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0689 mL 10.3443 mL 20.6885 mL
5 mM 0.4138 mL 2.0689 mL 4.1377 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.17 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

COA (254 KB) HNMR (301 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1][2]

Excitation of NucPE1-loaded cells at 514 nm is carried out with an Ar laser and emission is collected using a META detector between 522–554 nm. Excitation of Hoechst 33342 is carried out using a MaiTai two-photon laser at 780-nm pulses and emission is collected between 436–501 nm. All images in an experiment are collected simultaneously using identical microscope settings. Image analysis is performed in Image J. The background fluorescence is measured in a data set by selecting an ROI outside of the cells. This background number is then used to set the threshold of the image and thereby select for NucPE1 signal. The mean fluorescence intensity of this signal is then measured and utilized as the fluorescent signal for that particular image. The same threshold settings are utilized for all images in an experiment[1]. The measurement of nuclear H2O2 is achieved using NucPE1. The cells are incubated for 45 min at 10 μM NucPE1 in the dark. The incubation is followed by ishing and analysis by flow cytometry as explained above. Mitochondrial ROS are measured using MitoSOX probe. The cells are incubated with 5 μM MitoSOX for 15 min in the dark. The incubation is followed by ishing and analysis by flow cytometry[2]. Live imaging of the NucPE1 probe is carried out using excitation at 488 nm with an argon laser, and emission is collected using a META detector at about 520 nm. The Hoechst dye is incubated together with NucPE1. When multiple staining of NucPE1 and PO1 is performed, the multitracking mode of scanning is applied for acquisition of the images[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0689 mL 10.3443 mL 20.6885 mL 51.7213 mL
5 mM 0.4138 mL 2.0689 mL 4.1377 mL 10.3443 mL
10 mM 0.2069 mL 1.0344 mL 2.0689 mL 5.1721 mL
15 mM 0.1379 mL 0.6896 mL 1.3792 mL 3.4481 mL
20 mM 0.1034 mL 0.5172 mL 1.0344 mL 2.5861 mL
25 mM 0.0828 mL 0.4138 mL 0.8275 mL 2.0689 mL
30 mM 0.0690 mL 0.3448 mL 0.6896 mL 1.7240 mL
40 mM 0.0517 mL 0.2586 mL 0.5172 mL 1.2930 mL
50 mM 0.0414 mL 0.2069 mL 0.4138 mL 1.0344 mL
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NucPE1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NucPE11404091-23-1Nuclear Peroxy Emerald 1NucPE 1NucPE-1Nuclear Peroxy Emerald1Nuclear Peroxy Emerald-1Reactive Oxygen SpeciesInhibitorinhibitorinhibit

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