OVA Peptide (323-339)

Name: OVA Peptide (323-339)
Brand: MedChemExpress
SKU: HY-P0286
Rating: 4.5 (4 reviews)

Cat. No.: HY-P0286 Purity: 98.72%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

OVA Peptide (323-339) represents a T and B cell epitope of Ovalbumin (Ova), which is important in the generation and development of immediate hypersensitivity responses in BALB/c mice.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

OVA Peptide (323-339) Chemical Structure

CAS No. : 92915-79-2

Size	Price	Stock	Quantity
1 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 4 publication(s) in Google Scholar

4 Publications Citing Use of MCE OVA Peptide (323-339)

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

OVA Peptide (323-339) represents a T and B cell epitope of Ovalbumin (Ova), which is important in the generation and development of immediate hypersensitivity responses in BALB/c mice.

In Vitro

When pulsed with 0.01 μM of OVA Peptide (323-339), M2-expressing B cells lead to an increase in the number of T_H cells mobilizing calcium, compared to M2Y-expressing B cells. To assess if M2-expressing B cells are also able to promote stronger individual responses, we quantified the 405/530 ratio MFI of responding T_H cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

OVA Peptide (323-339) represents a T and B cell epitope of OVA, which is important in the generation and development of immediate hypersensitivity responses in BALB/c mice. Daily aerosolization of OVA Peptide (323-339) for 20 minutes over a period of 10 days has been as effective in the stimulation of a serum anti-OVA IgE antibody response as sensitization to native OVA by the same route. After sensitization to native OVA, the majority of the IgE anti-OVA response is directed against OVA Peptide (323-339). Evaluated by hematoxylin/eosin and major basic protein immunohistochemical stainings, OVA and OVA Peptide (323-339) induce similar lung inflammation. Interestingly, significant serum total IgE and OVA-specific IgE are observed in OVA mice when compared to saline control. OVA Peptide (323-339) mice show higher serum OVA-specific IgE, OVA Peptide (323-339)-specific IgE, IL-4 and lower IFN-γ similar to OVA mice. The proliferative response to OVA is found in cultured splenocytes of both OVA and OVA Peptide (323-339) mice, while the similar proliferative response to OVA Peptide (323-339) is only observed in the splenocytes of OVA Peptide (323-339)-sensitized and challenged mice. Although OVA Peptide (323-339) induces a Th2-like response in the mouse model as does OVA, OVA Peptide (323-339) has clearly limited immunogenic potency to activate OVA-sensitized and challenged mice splenocytes, unlike OVA^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1773.90

Formula

C₇₄H₁₂₀N₂₆O₂₅

CAS No.

92915-79-2

Appearance

Solid

Color

White to off-white

Sequence

Ile-Ser-Gln-Ala-Val-His-Ala-Ala-His-Ala-Glu-Ile-Asn-Glu-Ala-Gly-Arg

Sequence Shortening

ISQAVHAAHAEINEAGR

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : ≥ 50 mg/mL (28.19 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.5637 mL	2.8186 mL	5.6373 mL
5 mM	0.1127 mL	0.5637 mL	1.1275 mL
10 mM	0.0564 mL	0.2819 mL	0.5637 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (28.19 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.72%

Select Batch:

Data Sheet (278 KB) SDS (252 KB)

COA (244 KB) RP-HPLC (205 KB) MS (75 KB)

Handling Instructions (2659 KB)

References

[1]. Fontinha D, et al. Murid Gammaherpesvirus Latency-Associated Protein M2 Promotes the Formation of Conjugates between Transformed B Lymphoma Cells and T Helper Cells. PLoS One. 2015 Nov 6;10(11):e0142540. [Content Brief]

[2]. Sun LZ, et al. Comparison between ovalbumin and ovalbumin peptide 323-339 responses in allergic mice: humoral and cellular aspects. Scand J Immunol. 2010 May;71(5):329-35. [Content Brief]

Cell Assay ^[1]	A20 B cell lines are loaded, or not, with different concentrations of OVA Peptide (323-339) overnight. Cells are then conjugated with purified mouse CD4⁺ T cells and incubated for 20 h. After the 20 h incubation period, supernatant is recovered and stored at -20°C. IFN-γ production is quantified in the supernatants by sandwich ELISA. 96-well plates are analyzed. T cells cultured for two to four days in the presence of 3 μg/mL of anti-CD3 antibody are used as positive control of T cell activation. B cells cultured for 48 h in the presence of 2.5 μg/mL of anti-CD40 antibody and 5 μg/mL of the F(ab’)2/F(ab) portion of an anti-mouse IgG antibody are used as positive control for B cell activation^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[2]	Mice^[2] Fifty-one 8-week-old female BALB/c mice are randomly divided into three groups: OVA, OVA 323-339 and saline. They are intraperitoneally injected with 25 μg OVA or OVA Peptide (323-339) absorbed on 300 μg Alum or saline on days 0, 7, 14. On days 21-23, all groups are challenged intranasally with 20 μL of 1% OVA, 1% OVA Peptide (323-339) and saline, respectively. On days 0, 7, 14, mice are intraperitoneally injected with 25 μg OVA or OVA Peptide (323-339) absorbed on 300 μg Alum, or saline; on days 21-23, all groups are challenged intranasally with either 20 μL of 1% OVA, 1% OVA Peptide (323-339) or saline. On day 28, after killing, splenocytes are isolated and cultured under the stimulus of each allergen or medium. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Fontinha D, et al. Murid Gammaherpesvirus Latency-Associated Protein M2 Promotes the Formation of Conjugates between Transformed B Lymphoma Cells and T Helper Cells. PLoS One. 2015 Nov 6;10(11):e0142540. [Content Brief] [2]. Sun LZ, et al. Comparison between ovalbumin and ovalbumin peptide 323-339 responses in allergic mice: humoral and cellular aspects. Scand J Immunol. 2010 May;71(5):329-35. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	0.5637 mL	2.8186 mL	5.6373 mL	14.0932 mL
	5 mM	0.1127 mL	0.5637 mL	1.1275 mL	2.8186 mL
	10 mM	0.0564 mL	0.2819 mL	0.5637 mL	1.4093 mL
	15 mM	0.0376 mL	0.1879 mL	0.3758 mL	0.9395 mL
	20 mM	0.0282 mL	0.1409 mL	0.2819 mL	0.7047 mL
	25 mM	0.0225 mL	0.1127 mL	0.2255 mL	0.5637 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

OVA Peptide (323-339)92915-79-2OthersInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name			Lot #
Applicant Name *			Email Address *
Phone Number			Department *
Organization Name *			Country or Region *
Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

OVA Peptide (323-339)

Customer Review