1. GPCR/G Protein Neuronal Signaling Membrane Transporter/Ion Channel
  2. mAChR Potassium Channel
  3. Oxybutynin

Oxybutynin is an anticholinergic agent, which inhibits vascular Kv channels in a concentration-dependent manner, with an IC50 of 11.51 μM. Oxybutynin is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

Oxybutynin Chemical Structure

Oxybutynin Chemical Structure

CAS No. : 5633-20-5

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 66 In-stock
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Solid
100 mg USD 60 In-stock
500 mg USD 120 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Oxybutynin:

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Oxybutynin is an anticholinergic agent, which inhibits vascular Kv channels in a concentration-dependent manner, with an IC50 of 11.51 μM[1]. Oxybutynin is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

In Vitro

Oxybutynin (0.1, 0.3, 1, 3, 10, 30, 100 μM; 200 ms) inhibits vascular Kv channels in a concentration-dependent manner independent of anticholinergic effect in coronary arterial smooth muscle cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Coronary arterial smooth muscle cells (from male New Zealand White rabbits)
Concentration: 10 μM
Incubation Time: 200 ms
Result: Rapidly inhibited the Kv current within 2 min and reduced the Kv current by 44% at +60 Mv.
Inhibited the Kv current by changing the gating properties of Kv channels.

Cell Viability Assay[1]

Cell Line: Coronary arterial smooth muscle cells (from male New Zealand White rabbits)
Concentration: 0.1, 0.3, 1, 3, 10, 30, 100 μM
Incubation Time: 200 ms
Result: Reduced the Kv current amplitude in a concentration-dependent manner, with an IC50 value of 11.51 μM.
In Vivo

Oxybutynin (27.2 mg/kg; p.o.; single) exhibits significant binding of mouse brain muscarinic receptors that about a 2-fold increase in Kd values for specific [3H]N-methylscopolamine binding 0.5 and 2 h later[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male ddY strain mice (9 to 13-week-old)[2].
Dosage: 27.2 mg/kg (76.1 µmol/kg)
Administration: Oral administration; single.
Result: Significant increased Kd values for specific [3H]NMS binding in mouse bladder with values of 54.7% and 40.6% when at 0.5 and 2 hours, respectively.
Significant increased Kd values for specific [3H]NMS binding in mouse cerebral cortex with values of 120% and 71.2% when at 0.5 and 2 hours, respectively.
Clinical Trial
Molecular Weight

357.49

Formula

C22H31NO3

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C(O)(C1=CC=CC=C1)C2CCCCC2)OCC#CCN(CC)CC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (279.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 50 mg/mL (139.86 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7973 mL 13.9864 mL 27.9728 mL
5 mM 0.5595 mL 2.7973 mL 5.5946 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

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C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.08 mg/mL (5.82 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (5.82 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.55%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.7973 mL 13.9864 mL 27.9728 mL 69.9320 mL
5 mM 0.5595 mL 2.7973 mL 5.5946 mL 13.9864 mL
10 mM 0.2797 mL 1.3986 mL 2.7973 mL 6.9932 mL
15 mM 0.1865 mL 0.9324 mL 1.8649 mL 4.6621 mL
20 mM 0.1399 mL 0.6993 mL 1.3986 mL 3.4966 mL
25 mM 0.1119 mL 0.5595 mL 1.1189 mL 2.7973 mL
30 mM 0.0932 mL 0.4662 mL 0.9324 mL 2.3311 mL
40 mM 0.0699 mL 0.3497 mL 0.6993 mL 1.7483 mL
50 mM 0.0559 mL 0.2797 mL 0.5595 mL 1.3986 mL
60 mM 0.0466 mL 0.2331 mL 0.4662 mL 1.1655 mL
80 mM 0.0350 mL 0.1748 mL 0.3497 mL 0.8742 mL
100 mM 0.0280 mL 0.1399 mL 0.2797 mL 0.6993 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Oxybutynin
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