1. Membrane Transporter/Ion Channel
  2. Proton Pump
  3. PF 03716556

PF 03716556 is a potent, selective, competitive and reversible acid pump (H+,K+-ATPase) antagonist with pIC50s of 6.026, 6.038 and 6.009 for porcine, canine, and human recombinant gastric H+,K+-ATPase, respectively. PF 03716556 is inactive against other receptors, ion channels, and enzymes. PF 03716556 has the potential for gastroesophageal reflux disease research.

For research use only. We do not sell to patients.

PF 03716556 Chemical Structure

PF 03716556 Chemical Structure

CAS No. : 928774-43-0

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 119 In-stock
Solution
10 mM * 1 mL in DMSO USD 119 In-stock
Solid
5 mg USD 69 In-stock
10 mg USD 108 In-stock
25 mg USD 216 In-stock
50 mg USD 408 In-stock
100 mg USD 768 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

PF 03716556 is a potent, selective, competitive and reversible acid pump (H+,K+-ATPase) antagonist with pIC50s of 6.026, 6.038 and 6.009 for porcine, canine, and human recombinant gastric H+,K+-ATPase, respectively. PF 03716556 is inactive against other receptors, ion channels, and enzymes. PF 03716556 has the potential for gastroesophageal reflux disease research[1].

IC50 & Target

pIC50: 6.026 (Porcine gastric H+,K+-ATPase), 6.038 (Canine gastric H+,K+-ATPase) and 6.009 (Recombinant gastric H+,K+-ATPase)[1]

In Vitro

In porcine ion-tight membrane vesicles, PF 03716556 inhibits H+,K+-ATPase activity in a concentration-dependent manner, with a pIC50 value of 7.095 at pH 7.4[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

PF 03716556 (1-10 mg/kg; intraduodenal administration; once; male Sprague-Dawley rats) treatment inhibits gastric acid secretion in a dose-dependent manner in rats[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats (250 -300 g) treated with Pentagastrin[1]
Dosage: 1 mg/kg, 3 mg/kg, 10 mg/kg
Administration: Intraduodenal administration; once
Result: Inhibited gastric acid secretion in a dose-dependent manner.
Molecular Weight

394.47

Formula

C22H26N4O3

CAS No.
Appearance

Solid

Color

White to yellow

SMILES

CC1=CN2C=C(C=C(C2=N1)N[C@H]3C4=C(C=CC=C4OCC3)C)C(N(CCO)C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 23 mg/mL (58.31 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5350 mL 12.6752 mL 25.3505 mL
5 mM 0.5070 mL 2.5350 mL 5.0701 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5350 mL 12.6752 mL 25.3505 mL 63.3762 mL
5 mM 0.5070 mL 2.5350 mL 5.0701 mL 12.6752 mL
10 mM 0.2535 mL 1.2675 mL 2.5350 mL 6.3376 mL
15 mM 0.1690 mL 0.8450 mL 1.6900 mL 4.2251 mL
20 mM 0.1268 mL 0.6338 mL 1.2675 mL 3.1688 mL
25 mM 0.1014 mL 0.5070 mL 1.0140 mL 2.5350 mL
30 mM 0.0845 mL 0.4225 mL 0.8450 mL 2.1125 mL
40 mM 0.0634 mL 0.3169 mL 0.6338 mL 1.5844 mL
50 mM 0.0507 mL 0.2535 mL 0.5070 mL 1.2675 mL
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PF 03716556 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
PF 03716556
Cat. No.:
HY-13100
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