1. PF-4878691

PF-4878691  (Synonyms: 3M-852A)

Cat. No.: HY-100176 Purity: 99.64%
COA Handling Instructions

PF-4878691 (3M-852A) is an orally active TLR7 agonist. PF-4878691 has the innate immune response activity, antiviral efficacy against HCV, and can be used for the research of cancer.

For research use only. We do not sell to patients.

PF-4878691 Chemical Structure

PF-4878691 Chemical Structure

CAS No. : 532959-63-0

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 440 In-stock
Solution
10 mM * 1 mL in DMSO USD 440 In-stock
Solid
1 mg USD 150 In-stock
5 mg USD 400 In-stock
10 mg USD 640 In-stock
25 mg USD 1250 In-stock
50 mg USD 2000 In-stock
100 mg USD 3200 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Description

PF-4878691 (3M-852A) is an orally active TLR7 agonist. PF-4878691 has the innate immune response activity, antiviral efficacy against HCV, and can be used for the research of cancer[1][2].

IC50 & Target

TLR7[1]

In Vitro

PF-4878691 (10 μM, 4 h) induces a complex transcription network responsible for activating plasmacytoid dendritic cells for innate antiviral immune responses with optimized responses towards RNA viruses, increases co-stimulatory capacity, and increases survival in plasmacytoid dendritic cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

PF-4878691 (10-150 mg, Oral gavage, single dose) induces pharmacology in BALB/c mice and C57bl/6 J mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c mice , C57bl/6 J mice[3]
Dosage: 30 mg/kg, 60 mg/kg, 90 mg/kg, 150 mg/kg
Administration: Oral gavage (p.o.)
Result: Induced dose and time dependant lymphopenia and 2,5,oligoadenylate synthetase (2,5,OAS).
Caused cardiovascular changes.
Significantly increased TLR7 receptor RNA.
Molecular Weight

361.46

Formula

C17H23N5O2S

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CS(=O)(NCCCCN1C(CC)=NC2=C1C3=CC=CC=C3N=C2N)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 19.23 mg/mL (53.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7666 mL 13.8328 mL 27.6656 mL
5 mM 0.5533 mL 2.7666 mL 5.5331 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

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C2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.92 mg/mL (5.31 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.92 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (19.2 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.92 mg/mL (5.31 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.92 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (19.2 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.89%

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7666 mL 13.8328 mL 27.6656 mL 69.1639 mL
5 mM 0.5533 mL 2.7666 mL 5.5331 mL 13.8328 mL
10 mM 0.2767 mL 1.3833 mL 2.7666 mL 6.9164 mL
15 mM 0.1844 mL 0.9222 mL 1.8444 mL 4.6109 mL
20 mM 0.1383 mL 0.6916 mL 1.3833 mL 3.4582 mL
25 mM 0.1107 mL 0.5533 mL 1.1066 mL 2.7666 mL
30 mM 0.0922 mL 0.4611 mL 0.9222 mL 2.3055 mL
40 mM 0.0692 mL 0.3458 mL 0.6916 mL 1.7291 mL
50 mM 0.0553 mL 0.2767 mL 0.5533 mL 1.3833 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
PF-4878691
Cat. No.:
HY-100176
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