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QS11 

Cat. No.: HY-12762 Purity: >98.0%
Data Sheet SDS Handling Instructions

QS11 is a GTPase activating protein of ADP-ribosylation factor 1 (ARFGAP1) inhibitor.

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QS11 Chemical Structure

QS11 Chemical Structure

CAS No. : 944328-88-5

Size Price Stock Quantity
10 mM * 1 mL in DMSO $112 In-stock
5 mg $90 In-stock
10 mg $160 In-stock
25 mg $360 In-stock
50 mg $640 In-stock
100 mg $1100 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Description

QS11 is a GTPase activating protein of ADP-ribosylation factor 1 (ARFGAP1) inhibitor.

In Vitro

QS11 binds and inhibits the GTPase activating protein of ADP-ribosylation factor 1 (ARFGAP1), suggesting that QS11 modulates Wnt/β-catenin signaling through an effect on protein trafficking. QS11 (2.5 μM) activates the Super(8X)TOPFlash reporter 200-fold in the presence of Wnt-3a CM, whereas Wnt-3a treatment alone increases reporter activity 40-fold. QS11 increases reporter activity only 2-fold in the absence of Wnt-3a. QS11 shows potent activity (EC50=0.5 μM) with little cytotoxicity toward HEK293 and human primary fibroblast cells. QS11 effectively reduces in vitro migration of metastatic human breast cancer cells[1].

References
Preparing Stock Solutions
Concentration Volume (DMSO) Mass 1 mg 5 mg 10 mg
1 mM 1.7616 mL 8.8078 mL 17.6156 mL
5 mM 0.3523 mL 1.7616 mL 3.5231 mL
10 mM 0.1762 mL 0.8808 mL 1.7616 mL
Cell Assay
[1]

ARF-GTP levels are measured by using the GGA binding assay. NIH 3T3 cells are treated with QS11 or QS11-NC at the indicated concentrations for 36 h. Cells are lysed in ARF assay lysis buffer [50 mM Tris·HCl (pH 7.5), 100 mM NaCl, 2 mM MgCl2, 0.1% SDS, 0.5% sodium deoxycholate, 1% Triton X-100, 10% glycerol, and protease inhibitors]. GTP-bound ARF is assayed by its binding to a GST fusion protein, which contains the VHS domain to the GAT region of an ARF effector GGA3[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

567.68

Formula

C₃₆H₃₃N₅O₂

CAS No.

944328-88-5

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: 10 mM

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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QS11
Cat. No.:
HY-12762
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