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Quin-2AM  (Synonyms: Quin-2 acetoxymethyl ester)

Cat. No.: HY-101902
Handling Instructions

Quin-2AM is a fluorecent Ca2+ chelator, with high affinity for calcium. Quin-2AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence.

For research use only. We do not sell to patients.

Quin-2AM Chemical Structure

Quin-2AM Chemical Structure

CAS No. : 83104-85-2

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Based on 1 publication(s) in Google Scholar

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Description

Quin-2AM is a fluorecent Ca2+ chelator, with high affinity for calcium. Quin-2AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence[1].

In Vitro

1. Preparation of Quin-2AM working solution
1.1 Preparation of the stock solution
Dissolve 1 mg Quin-2AM in 135 μL DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20℃or -80℃away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Quin-2AM working solution
Dilute the stock solution in HBSS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of Quin-2AM working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4℃for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

829.76

Formula

C38H43N3O18

CAS No.
Emission (Em)

492

Excitation (Ex)

339

SMILES

O=C(OCOC(C)=O)CN(CC(OCOC(C)=O)=O)C1=CC=C(C)C=C1OCC2=NC3=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C(OC)C=C3C=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Quin-2AM Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

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This equation is commonly abbreviated as: C1V1 = C2V2

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Quin-2AM
Cat. No.:
HY-101902
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