RF9

Name: RF9
Brand: MedChemExpress
SKU: HY-107382
Rating: 4.5 (1 reviews)

Cat. No.: HY-107382 Purity: 98.04%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

RF9 is a potent and selective Neuropeptide FF receptor antagonist, with K_i values of 58 and 75 nM for hNPFF1R and hNPFF2R, respectively.

For research use only. We do not sell to patients.

RF9 Chemical Structure

CAS No. : 876310-60-0

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 242	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 242	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 108	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 228	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of RF9:

RF9 hydrochloride In-stock

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Description

RF9 is a potent and selective Neuropeptide FF receptor antagonist, with K_i values of 58 and 75 nM for hNPFF1R and hNPFF2R, respectively^[1]^[2].

IC₅₀ & Target^[1]

hNPFF1R

58 nM (Ki)

hNPFF2R

75 nM (Ki)

In Vitro

RF9 (10 μM) pretrement completely blocks NPFF induced neurite outgrowth of Neuro 2A cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

RF9 (0.1 mg/kg, s.c. ) with heroin coadministration prevents heroin-induced delayed hyperalgesia and tolerance^[1].
RF9 (10 μg) infused alone does not result in a significant alteration of MAP or heart rate. Conversely, MAP and heart rate increases evoked by NPFF are significantly blocked when NPFF is applied in conjunction with RF9^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Rats^[1].
Dosage:	0.1 mg/kg.
Administration:	S.C. 30 min before 0.3 mg/kg heroin or saline on basal nociceptive threshold in rats.
Result:	Opposed to delaye heroin-induced hyperalgesia and associated tolerance.

Molecular Weight

482.62

Formula

C₂₆H₃₈N₆O₃

CAS No.

876310-60-0

Appearance

Solid

Color

White to off-white

SMILES

NC([C@H](CC1=CC=CC=C1)NC([C@H](CCCNC(N)=N)NC(C23C[C@H](C4)C[C@H](C[C@H]4C3)C2)=O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (259.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 16.67 mg/mL (34.54 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0720 mL	10.3601 mL	20.7202 mL
5 mM	0.4144 mL	2.0720 mL	4.1440 mL
10 mM	0.2072 mL	1.0360 mL	2.0720 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 10 mg/mL (20.72 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.04%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (252 KB) LCMS (258 KB)

Handling Instructions (2659 KB)

References

[1]. Simonin F, et al. RF9, a potent and selective neuropeptide FF receptor antagonist, prevents opioid-induced tolerance associated with hyperalgesia. Proc Natl Acad Sci U S A. 2006 Jan 10;103(2):466-71. [Content Brief]

[2]. Ting Zhang, et al. Discovery of Two Novel Branched Peptidomimetics Containing endomorphin-2 and RF9 Pharmacophores: Synthesis and Neuropharmacological Evaluation. Bioorg Med Chem. 2019 Feb 15;27(4):630-643. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.0720 mL	10.3601 mL	20.7202 mL	51.8006 mL
	5 mM	0.4144 mL	2.0720 mL	4.1440 mL	10.3601 mL
	10 mM	0.2072 mL	1.0360 mL	2.0720 mL	5.1801 mL
	15 mM	0.1381 mL	0.6907 mL	1.3813 mL	3.4534 mL
	20 mM	0.1036 mL	0.5180 mL	1.0360 mL	2.5900 mL
	25 mM	0.0829 mL	0.4144 mL	0.8288 mL	2.0720 mL
	30 mM	0.0691 mL	0.3453 mL	0.6907 mL	1.7267 mL
DMSO	40 mM	0.0518 mL	0.2590 mL	0.5180 mL	1.2950 mL
	50 mM	0.0414 mL	0.2072 mL	0.4144 mL	1.0360 mL
	60 mM	0.0345 mL	0.1727 mL	0.3453 mL	0.8633 mL
	80 mM	0.0259 mL	0.1295 mL	0.2590 mL	0.6475 mL
	100 mM	0.0207 mL	0.1036 mL	0.2072 mL	0.5180 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

RF9 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RF9876310-60-0RF 9RF-9Neuropeptide Y ReceptorNPY receptorInhibitorinhibitorinhibit

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RF9

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