Ribocil-C Racemate

Cat. No.: HY-19488: FAQs
Data Sheet SDS COA Handling Instructions

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Ribocil-C Racemate is the racemate of Ribocil-C. Ribocil-C is a highly selective inhibitor of bacterial riboflavin riboswitches.

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Ribocil-C Racemate Chemical Structure

CAS No. : 2309762-18-1

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Other Forms of Ribocil-C Racemate:

Ribocil In-stock
Ribocil-C In-stock
Ribocil-C (R enantiomer) In-stock

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Ribocil-C Racemate is the racemate of Ribocil-C. Ribocil-C is a highly selective inhibitor of bacterial riboflavin riboswitches.

IC₅₀ & Target

Bacterial riboflavin riboswitches^[1]

In Vitro

Ribocil-C is a highly selective inhibitor of the flavin mononucleotide (FMN) riboswitch that controls expression of de novo riboflavin (RF, vitamin B2) biosynthesis in Escherichia coli. Ribocil-C specifically inhibits dual FMN riboswitches, separately controlling RF biosynthesis and uptake processes essential for Staphylococcus aureus growth and pathogenesis^[1]. Ribocil-C is a small-molecule synthetic mimic of FMN that binds the FMN riboswitch of multiple GN bacteria, including Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii, to inhibit ribB expression, RF synthesis, and consequently arrest bacterial growth^[1]^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Higher dose Ribocil-C treatment groups (60 and 120 mg kg21 ribocil-C) demonstrate a dose-dependent reduction in bacterial burden of 1.87 and 3.29 log₁₀[CFU per g spleen] reduction respectively versus shamtreated mice, without mortality or gross effects of toxicity observed^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

419.50

Formula

C₂₁H₂₁N₇OS

CAS No.

2309762-18-1

Appearance

Solid

Color

White to off-white

SMILES

O=C1C=C(C2=CC=CS2)NC(C3CCCN(CC4=CN(C5=NC=CC=N5)C=N4)C3)=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 30 mg/mL (71.51 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3838 mL	11.9190 mL	23.8379 mL
5 mM	0.4768 mL	2.3838 mL	4.7676 mL
10 mM	0.2384 mL	1.1919 mL	2.3838 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (247 KB) HNMR (205 KB) LCMS (513 KB)

Handling Instructions (2659 KB)

References

[1]. Wang H, et al. Dual-Targeting Small-Molecule Inhibitors of the Staphylococcus aureus FMN Riboswitch DisruptRiboflavin Homeostasis in an Infectious Setting. Cell Chem Biol. 2017 May 18;24(5):576-588. [Content Brief]

[2]. Howe JA, et al. Selective small-molecule inhibition of an RNA structural element. Nature. 2015 Oct 29;526(7575):672-7. [Content Brief]

Animal Administration
^[2]

DBA/2J mice are infected by intraperitoneal injectionwith Escherichia coli strain MB5746 (5×10⁴ CFU per mouse) and treated by subcutaneous injection with Ribocil-C (30, 60, 120 mg/kg) or ciprofloxacin (0.5mg/kg) three times over a 24 h infection period. Spleens are aseptically collected from five mice per group and the reduction of log[CFU per g spleen tissue] is calculated on the basis of bacterial burden in spleens of the vehicle-treated (10% DMSO) control group^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Wang H, et al. Dual-Targeting Small-Molecule Inhibitors of the Staphylococcus aureus FMN Riboswitch DisruptRiboflavin Homeostasis in an Infectious Setting. Cell Chem Biol. 2017 May 18;24(5):576-588. [Content Brief]

[2]. Howe JA, et al. Selective small-molecule inhibition of an RNA structural element. Nature. 2015 Oct 29;526(7575):672-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3838 mL	11.9190 mL	23.8379 mL	59.5948 mL
	5 mM	0.4768 mL	2.3838 mL	4.7676 mL	11.9190 mL
	10 mM	0.2384 mL	1.1919 mL	2.3838 mL	5.9595 mL
	15 mM	0.1589 mL	0.7946 mL	1.5892 mL	3.9730 mL
	20 mM	0.1192 mL	0.5959 mL	1.1919 mL	2.9797 mL
	25 mM	0.0954 mL	0.4768 mL	0.9535 mL	2.3838 mL
	30 mM	0.0795 mL	0.3973 mL	0.7946 mL	1.9865 mL
	40 mM	0.0596 mL	0.2980 mL	0.5959 mL	1.4899 mL
	50 mM	0.0477 mL	0.2384 mL	0.4768 mL	1.1919 mL
	60 mM	0.0397 mL	0.1986 mL	0.3973 mL	0.9932 mL