Robenidine hydrochloride

Name: Robenidine hydrochloride
Brand: MedChemExpress
SKU: HY-B2157
Rating: 4.5 (1 reviews)

Cat. No.: HY-B2157 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Robenidine hydrochloride is an anticoccidial agent which is also active against MRSA and VRE with MIC₅₀s of 8.1 and 4.7 μM, respectively.

For research use only. We do not sell to patients.

Robenidine hydrochloride Chemical Structure

CAS No. : 25875-50-7

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 66	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 66	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Robenidine hydrochloride:

Robenidine-d₈ hydrochloride In-stock

1 Publications Citing Use of MCE Robenidine hydrochloride

•Patent. US20230147129A1.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Robenidine hydrochloride is an anticoccidial agent which is also active against MRSA and VRE with MIC₅₀s of 8.1 and 4.7 μM, respectively.

IC₅₀ & Target

Coccidia

In Vitro

Robenidine (compound 1) inhibits the growth of MRSA and VRE with MIC values of 8.1 and 4.7 μM, respectively. Robenidine is bactericidal against all of the S. aureus strains tested with MBC/MIC₉₀ ratios ≤2. A profound and negative impact on the MIC values with a 4-fold decrease with Robenidine at 2% serum and no activity at 50% serum is observed^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The time that the mean plasma concentration exceeds the concentration of 1 µg/mL is approximately 6 h in the Florfenicol (FFC) alone group; however, it is lowered to 4 h by Robenidine (ROB) pretreatment. The terminal elimination half-life (t_1/2z), area under the concentration-time curve (AUC), area under the first moment curve (AUMC), and mean residence time (MRT) significantly decreased, whereas the elimination rate constant (λ_z) and total body clearance (CL_z) obviously increased in rabbits pretreated with Robenidine^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

370.66

Formula

C₁₅H₁₄Cl₃N₅

CAS No.

25875-50-7

Appearance

Solid

Color

White to off-white

SMILES

N=C(N/N=C/C1=CC=C(Cl)C=C1)N/N=C/C2=CC=C(Cl)C=C2.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 6.25 mg/mL (16.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6979 mL	13.4895 mL	26.9789 mL
5 mM	0.5396 mL	2.6979 mL	5.3958 mL
10 mM	0.2698 mL	1.3489 mL	2.6979 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.62 mg/mL (1.67 mM); Clear solution

This protocol yields a clear solution of ≥ 0.62 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.2 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.62 mg/mL (1.67 mM); Clear solution

This protocol yields a clear solution of ≥ 0.62 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.2 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (273 KB) SDS (622 KB)

COA (244 KB)

Handling Instructions (2659 KB)

References

[1]. Abraham RJ, et al. Robenidine Analogues as Gram-Positive Antibacterial Agents. J Med Chem. 2016 Mar 10;59(5):2126-38. [Content Brief]

[2]. Liu C, et al. Influence of three coccidiostats on the pharmacokinetics of florfenicol in rabbits. Exp Anim. 2015;64(1):73-9. [Content Brief]

Cell Assay ^[1]	MRSA clinical isolates are used in this study and the MIC₅₀ for Robenidine (compound 1) is determined using a modified MIC assay. The antimicrobial dilutions of Robenidine are completed in 100% DMSO, with 2 μL added to each well. The assay is performed in a total volume of 200 μL in 96-well plates. Robenidine is tested in final concentrations of 0.7 to 345.3 μM. Plates are incubated for 20 to 24 h at 37°C before determination of the MIC^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[2]	The rabbits are divided into four groups (n=8, each group). The rabbits in the control group are fed anticoccidial-free rations throughout the study. Rabbits in the other group are fed rations containing Robenidine (ROB) (66 ppm) for 20 consecutive days. At the end of the 20th day of feeding, a single dose of Florfenicol (FFC) is injected intravenously at 25 mg/kg body weight (b.w.) into the left auricular vein of each rabbit in all groups. Blood (approximately 1 mL) samples are collected into heparin-coated tubes from the right auricular vein of each rabbit at 5, 10, 15, 30, and 45 min and 1, 1.5, 2, 4, 6, 8, and 12 h after administration of FFC. The plasma is harvested after centrifugation at 3,000 g for 10 min and stored at -20°C until analysis^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Abraham RJ, et al. Robenidine Analogues as Gram-Positive Antibacterial Agents. J Med Chem. 2016 Mar 10;59(5):2126-38. [Content Brief] [2]. Liu C, et al. Influence of three coccidiostats on the pharmacokinetics of florfenicol in rabbits. Exp Anim. 2015;64(1):73-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6979 mL	13.4895 mL	26.9789 mL	67.4473 mL
	5 mM	0.5396 mL	2.6979 mL	5.3958 mL	13.4895 mL
	10 mM	0.2698 mL	1.3489 mL	2.6979 mL	6.7447 mL
	15 mM	0.1799 mL	0.8993 mL	1.7986 mL	4.4965 mL