1. Cell Cycle/DNA Damage
    Epigenetics
    Autophagy
  2. Sirtuin
    Autophagy

Sirtinol 

Cat. No.: HY-13515 Purity: >98.0%
Handling Instructions

Sirtinol is a sirtuin inhibitor, with IC50s of 48 μM, 57.7 μM and 131 μM for ySir2, hSIRT2 and hSIRT2, respectively.

For research use only. We do not sell to patients.
Sirtinol Chemical Structure

Sirtinol Chemical Structure

CAS No. : 410536-97-9

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 73 In-stock
5 mg USD 66 In-stock
10 mg USD 102 In-stock
50 mg USD 408 In-stock
100 mg USD 696 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Sirtinol is a sirtuin inhibitor, with IC50s of 48 μM, 57.7 μM and 131 μM for ySir2, hSIRT2 and hSIRT2, respectively.

IC50 & Target

IC50: 48 μM (ySir2), 57.7 μM (hSIRT2), 131 μM (hSIRT1)[4]

In Vitro

Sirtinol reduces the growth of MCF-7 cells in a concentration- and time-dependent manner. The IC50 values of sirtinol are 48.6 μM and 43.5 μM after 24 and 48 h of treatment, respectively. Sirtinol significantly decreases SIRT1 expression and increases the acetylated p53 level[1]. Sirtinol attenuates the proliferation and induces apoptosis of nonsmall cell lung cancer (NSCLC) H1299 cells and causes the significantly increased level of FoxO3a, a proapoptotic transcription factor targeted by Sirt1[2].

In Vivo

Sirtinol has anti-inflammatory effects through direct inhibition of HNE activity and attenuates HNE-induced and LPS-mediated tissue or organ injury[3].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 2.5350 mL 12.6752 mL 25.3505 mL
5 mM 0.5070 mL 2.5350 mL 5.0701 mL
10 mM 0.2535 mL 1.2675 mL 2.5350 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[2]

Sirtinol is dissolved in 100% DMSO at concentration of 10 mM and stored at −20°C until use. The cell proliferation of H1299 cells is determined by trypan blue dye exclusion assay. Human nonsmall cell lung cancer (NSCLC) cells are seeded in 12-well plates and treated with indicated concentrations of sirtinol (0, 10, 20, and 50 μM) for 24 h and 48 h, respectively. After incubation, the cells are stained by 0.2% trypan blue and counted by Countess[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[3]

Mice: 30 male mice (20–25 g, 7–8 weeks old) are used in this model. Briefly, mice are randomly divided into five groups; then mice are intraperitoneally injected with 50 μL DMSO (vehicle group) or sirtinol (2.5 or 5.0 mg/kg body weight). After 1 h, paw inflammation is induced . The thickness of the paw is measured before and after HNE or saline injection[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

394.47

Formula

C₂₆H₂₂N₂O₂

CAS No.

410536-97-9

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: 6.5 mg/mL (Need ultrasonic)

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Product Name:
Sirtinol
Cat. No.:
HY-13515
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