Sulforaphane

Name: Sulforaphane
Brand: MedChemExpress
SKU: HY-13755
Rating: 5.0 (38 reviews)

Cat. No.: HY-13755 Purity: 98.19%: FAQs
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Sulforaphane is an orally active inducer of the Keap1/Nrf2/ARE pathway. Sulforaphane promotes the transcription of tumor-suppressing proteins and effectively inhibits the activity of HDACs. Through the activation of the Keap1/Nrf2/ARE pathway and further induction of HO-1 expression, Sulforaphane protects the heart. Sulforaphane suppresses high glucose-induced pancreatic cancer through AMPK-dependent signal transmission. Sulforaphane exhibits both anticancer and anti-inflammatory properties.

For research use only. We do not sell to patients.

Sulforaphane Chemical Structure

CAS No. : 4478-93-7

Size	Price	Stock	Quantity
5 mg	USD 38	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 135	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 420	In-stock	Estimated Time of Arrival: December 31
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Based on 38 publication(s) in Google Scholar

Other Forms of Sulforaphane:

(R)-Sulforaphane In-stock

33 Publications Citing Use of MCE Sulforaphane

: Sulforaphane purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2021 Aug 7;174412. [Abstract]; Western blotting analysis shows that CIBP can significantly induce the protein expression of Nrf2 and HO-1 in the spinal cord and that intrathecal administration of Sulforaphane (SFN) can further promoted the protein expression of Nrf2 and HO-1 protein.

: Sulforaphane purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2021 Aug 7;174412. [Abstract]; Immunofluorescence histochemistry confirmes that Sulforaphane (SFN) treatment further increased Nrf2 and HO-1 expression in the dorsal horn of the spinal cord of CIBP rats.

: Sulforaphane purchased from MedChemExpress. Usage Cited in: Theranostics. 2020 Jun 5;10(16):7319-7334. [Abstract]; Western blot detection of Nrf2, TLR4, IRF1, iNOS, and ARG-1 levels following Sulforaphane (SFN; 10 μM or 20 μM) treatment or Nrf2 upregulation/downregulation in BMDMs co-cultured with COM-stimulated TECs. SFN treatment activates Nrf2 expression, which then downregulates the expression of TLR4, IRF1, and iNOS and upregulates the expression of ARG-1.

: Sulforaphane purchased from MedChemExpress. Usage Cited in: Theranostics. 2020 Jun 5;10(16):7319-7334. [Abstract]; The distribution of iNOS (green) and ARG-1 (red) in BMDMs according to immunofluorescence. Sulforaphane (SFN; 10 μM or 20 μM) decreases iNOS and increases ARG-1 levels.

: Sulforaphane purchased from MedChemExpress. Usage Cited in: Vascul Pharmacol. 2018 Oct;109:56-71. [Abstract]; A representative western blot analysis gel showing the reduces abundance of eNOS relative to GAPDH in tissues cultured under hyperglycaemic conditions (25 mM glucose: 25) versus either freshly isolated tissues (F, first lane on left) or tissues cultured under euglycaemic conditions (10 mM glucose: 10). The abundance of eNOS is preserved for tissues cultured in 25 mM glucose along with 1 μM Sulforaphane (25+S).

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HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

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Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

HDAC

Bax

Caspase-3

In Vitro

Sulforaphane (0-30 μM) induces cell cycle arrest and apoptosis in a dose-dependent manner. Sulforaphane-induced cell cycle arrest is associated with an increase in the expression of cyclin A and B1^[1].
Sulforaphane (0-30 μM) inhibits the re-initiation of growth and decreases cell viability in HT29 cells, exhibiting lower toxicity towards differentiated cells^[1].
Sulforaphane (10 μM, 24 hours) pre-treatment reduces the number of apoptotic cells, decreases the expression of pro-apoptotic proteins (Bax, caspase-3, cytochrome c), and counteracts the increase in mitochondrial membrane potential induced by Doxorubicin (HY-15142A) (1 μM, 2 hours) in H9c2 cells^[2]. Sulforaphane (10 μM, 2 or 24 hours) effectively reduces ROS production and cell apoptosis in H9c2 cells induced by Doxorubicin (1 μM, 2 or 24 hours) through the activation of the Keap1/Nrf2/ARE pathway and further induction of HO-1 expression^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	H9c2 cells
Concentration:	Sulforaphane: 10 μM; Doxorubicin: 1 μM
Incubation Time:	Sulforaphane: 2h; Doxorubicin: 2, 24h
Result:	Prevented the release of cytochrome c into the cytosol. Prevented the translocation of Bax into the cytosol. Attenuated the doxorubicin-induced increase in the levels of cleaved caspase-3. Induced a significant increase in HO-1 protein expression. Induced a significantly higher level of Nrf2 expression in the nucleus compared to the cytoplasm.

Apoptosis Analysis^[2]

Cell Line:	H9c2 cells
Concentration:	Sulforaphane: 10 μM; Doxorubicin: 1 μM
Incubation Time:	Sulforaphane: 2h; Doxorubicin: 24h
Result:	Protected the H9c2 cells against doxorubicin-induced cell death. Increased cell viability in a dose-dependent manner. Significantly reduced the number of apoptotic cells treated with Doxorubicin.

RT-PCR^[2]

Cell Line:	H9c2 cells
Concentration:	Sulforaphane: 10 μM; Doxorubicin: 1 μM
Incubation Time:	Sulforaphane: 2, 24h; Doxorubicin: 2, 24h
Result:	Induced heme oxygenase-1 (HO-1) mRNA expression in a dose-dependent manner.

In Vivo

Sulforaphane (13.3, 17.7, 26.6 mg/kg; Oral gavage; 5 days) is capable of inhibiting the formation of mammary tumors in female Sprague-Dawley rats following a single-dose treatment with DMBA (HY-W011845) (8 mg/mL)^[3].
Sulforaphane (13.3, 17.7, 26.6 mg/kg; Oral gavage; 5 days) can reduce the incidence, multiplicity, and weight of mammary tumors induced by DMBA (8 mg/mL) in female Sprague-Dawley rats, and delay their development^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female Sprague-Dawley rat^[3]
Dosage:	Sulforaphane: 13.3, 17.7, 26.6 mg/kg; DMBA (HY-W011845): 8 mg/mL
Administration:	Oral gavage; 5 days
Result:	Prevented the occurrence of tumors in a dose-dependent manner. Significantly reduced the incidence of tumors.

Clinical Trial

Molecular Weight

177.29

Formula

C₆H₁₁NOS₂

CAS No.

4478-93-7

Appearance

Liquid

Color

Colorless to light yellow

SMILES

O=S(CCCCN=C=S)C

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : ≥ 62.5 mg/mL (352.53 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (282.02 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.6405 mL	28.2024 mL	56.4048 mL
5 mM	1.1281 mL	5.6405 mL	11.2810 mL
10 mM	0.5640 mL	2.8202 mL	5.6405 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (14.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 30 % SBE-β-CD
Solubility: 10 mg/mL (56.40 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.75%

Select Batch:

Data Sheet (277 KB) SDS (0 KB)

COA (242 KB) HNMR (191 KB) LCMS (139 KB)

Handling Instructions (2659 KB)

References

[1]. Gamet-Payrastre L, et al. Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Cancer Res. 2000 Mar 1;60(5):1426-33. [Content Brief]

[2]. Li B, et al. Sulforaphane prevents doxorubicin-induced oxidative stress and cell death in rat H9c2 cells. Int J Mol Med. 2015 Jul;36(1):53-64. [Content Brief]

[3]. Zhang Y, et al. Anticarcinogenic activities of sulforaphane and structurally related synthetic norbornylisothiocyanates. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3147-50. [Content Brief]

[4]. Chen X, et al. Activation of Nrf2 by Sulforaphane Inhibits High Glucose-Induced Progression of PancreaticCancer via AMPK Dependent Signaling. ell Physiol Biochem. 2018;50(3):1201-1215. [Content Brief]

[5]. De Nicola GR, et al. Novel gram-scale production of enantiopure R-sulforaphane from Tuscan black kale seeds. Molecules. 2014 May 27;19(6):6975-86. [Content Brief]

[6]. Abdull Razis AF, et al. The natural chemopreventive phytochemical R-sulforaphane is a far more potent inducer of the carcinogen-detoxifying enzyme systems in rat liver and lung than the S-isomer. Int J Cancer. 2011 Jun 15;128(12):2775-82. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	5.6405 mL	28.2024 mL	56.4048 mL	141.0119 mL
	5 mM	1.1281 mL	5.6405 mL	11.2810 mL	28.2024 mL
	10 mM	0.5640 mL	2.8202 mL	5.6405 mL	14.1012 mL
	15 mM	0.3760 mL	1.8802 mL	3.7603 mL	9.4008 mL
	20 mM	0.2820 mL	1.4101 mL	2.8202 mL	7.0506 mL
	25 mM	0.2256 mL	1.1281 mL	2.2562 mL	5.6405 mL
	30 mM	0.1880 mL	0.9401 mL	1.8802 mL	4.7004 mL
	40 mM	0.1410 mL	0.7051 mL	1.4101 mL	3.5253 mL
	50 mM	0.1128 mL	0.5640 mL	1.1281 mL	2.8202 mL
	60 mM	0.0940 mL	0.4700 mL	0.9401 mL	2.3502 mL
	80 mM	0.0705 mL	0.3525 mL	0.7051 mL	1.7626 mL
	100 mM	0.0564 mL	0.2820 mL	0.5640 mL	1.4101 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Sulforaphane Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sulforaphane4478-93-7HDACKeap1-Nrf2ApoptosisBcl-2 FamilyCaspaseReactive Oxygen SpeciesHistone deacetylasesBaxCaspase-3Cytochrome cHT29DoxorubicinMitochondrial membrane potentialInhibitorinhibitorinhibit

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Sulforaphane

Customer Review

Other Forms of Sulforaphane:

Sulforaphane Related Antibodies

33 Publications Citing Use of MCE Sulforaphane

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View All Caspase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Sulforaphane Related Antibodies

Molarity Calculator

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Complete Stock Solution Preparation Table

Sulforaphane Related Classifications

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