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  4. Thiamine monochloride

Thiamine monochloride  (Synonyms: Vitamin B1)

Cat. No.: HY-A0100 Purity: 99.88%
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Thiamine monochloride (Vitamin B1) is an essential vitamin that plays an important role in cellular production of energy from ingested food and enhances normal neuronal actives.

For research use only. We do not sell to patients.

Thiamine monochloride Chemical Structure

Thiamine monochloride Chemical Structure

CAS No. : 59-43-8

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in Water USD 55 In-stock
Solid
500 mg USD 50 In-stock
1 g USD 60 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Thiamine monochloride:

Thiamine monochloride Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Thiamine monochloride (Vitamin B1) is an essential vitamin that plays an important role in cellular production of energy from ingested food and enhances normal neuronal actives.

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

In Vitro

Thiamine levels in the blood of homozygous KO and KI mice feed a conventional diet are decreased to 0.058±0.051 and 0.126±0.092 μM, respectively, at 7 weeks compared to WT mice (0.796±0.259 μM). When WT and homozygous KO and KI mice are fed a thiamine-restricted diet (thiamine: 0.60 mg/100 g food), blood thiamine concentration at 5 and 14 days is markedly decreased to 0.010±0.009 and 0.010±0.006 μM, respectively, compared to WT mice (0.609±0.288 μM). Thiamine concentration in brain homogenate of WT mice fed a conventional diet is 3.81±2.18 nmol/g wet weight, and that of KO and KI is 1.33±0.96 and 2.16±1.55 nmol/g wet weight, respectively. Notably, thiamine concentration in brain homogenate decreased steadily in KO and KI mice fed a thiamine-restricted diet (thiamine: 0.60 mg/100 g food) for 5 days (0.95±0.72 nmol/g wet weight) and 14 days (1.11±0.24 nmol/g wet weight), respectively, compared to WT (3.65±1.02 nmol/g wet weight), before the mice presented an phenotype of disease[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Thiamine monochloride Related Antibodies
In Vivo

WT, homozygous, and heterozygous KO and KI mice feed a conventional diet (thiamine: 1.71 mg/100 g) survive for over 6 months without any phenotype of disease. Homozygous KO and KI mice feed a thiamine-restricted diet (thiamine: 0.60 mg/100 g food) showe paralysis, weight loss, and immobility, and die within 12 and 30 days, respectively. Similarly, homozygous KO and KI mice feed a thiamine-restricted diet with an even lower percentage of thiamine (thiamine: 0.27 mg/100 g food) die within 14 and 18 days, respectively. However, WT and heterozygous KO and KI mice feed a thiamine-restricted diet (thiamine: 0.60 mg or 0.27 mg/100g food) survive for over 6 months without any phenotype of disease[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

300.81

Formula

C12H17ClN4OS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1=C(CCO)SC=[N+]1CC2=CN=C(C)N=C2N.[Cl-]
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (332.44 mM)

DMSO : 1 mg/mL (3.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3244 mL 16.6218 mL 33.2436 mL
5 mM 0.6649 mL 3.3244 mL 6.6487 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: ≥ 100 mg/mL (332.44 mM); Clear solution

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.88%

COA (248 KB) LCMS (264 KB)

Handling Instructions (2659 KB)
References
Animal Administration
[2]

Slc19a3 E314Q KI mice are maintained routinely with conventional diet, which has a thiamine concentration (thiamine hydrochloride, MW=337.3) of 1.71 mg/100 g food. two types of thiamine-restricted food based on “purified diets for laboratory rodents” are prepared, in which thiamine concentration is 0.60 mg/100 g food (35% thiamine of conventional food) or 0.27 mg/100 g food (16% thiamine of conventional food). A high-thiamine-containing food is also prepared from AIN-93M, in which thiamine concentration is five times that of CE-2 (thiamine: 8.50 mg/100 g food). Thiamine concentration is determined at Japan Food Research Laboratories[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 3.3244 mL 16.6218 mL 33.2436 mL 83.1089 mL
H2O 5 mM 0.6649 mL 3.3244 mL 6.6487 mL 16.6218 mL
10 mM 0.3324 mL 1.6622 mL 3.3244 mL 8.3109 mL
15 mM 0.2216 mL 1.1081 mL 2.2162 mL 5.5406 mL
20 mM 0.1662 mL 0.8311 mL 1.6622 mL 4.1554 mL
25 mM 0.1330 mL 0.6649 mL 1.3297 mL 3.3244 mL
30 mM 0.1108 mL 0.5541 mL 1.1081 mL 2.7703 mL
40 mM 0.0831 mL 0.4155 mL 0.8311 mL 2.0777 mL
50 mM 0.0665 mL 0.3324 mL 0.6649 mL 1.6622 mL
60 mM 0.0554 mL 0.2770 mL 0.5541 mL 1.3851 mL
80 mM 0.0416 mL 0.2078 mL 0.4155 mL 1.0389 mL
100 mM 0.0332 mL 0.1662 mL 0.3324 mL 0.8311 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Thiamine monochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Thiamine monochloride59-43-8Vitamin B1Vitamin B 1Vitamin B-1Endogenous MetaboliteBacterialInhibitorinhibitorinhibit

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